ID PAC11_YEAST Reviewed; 533 AA.
AC P40960; D6VTB1; Q03398;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 27-MAR-2024, entry version 177.
DE RecName: Full=WD repeat-containing protein PAC11;
GN Name=PAC11; OrderedLocusNames=YDR488C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Hoyt M.A.;
RT "Redundant pathways of mitotic motor function revealed by synthetic-lethal-
RT with-cin8 mutants.";
RL Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP INTERACTION WITH NUM1.
RX PubMed=11266443; DOI=10.1083/jcb.152.2.251;
RA Farkasovsky M., Kuentzel H.;
RT "Cortical Num1p interacts with the dynein intermediate chain Pac11p and
RT cytoplasmic microtubules in budding yeast.";
RL J. Cell Biol. 152:251-262(2001).
CC -!- FUNCTION: Required for viability in the absence of the kinesin-related
CC CIN8 mitotic motor. May be a dynein intermediate chain.
CC -!- SUBUNIT: Interacts with NUM1, when DYN1 is present.
CC {ECO:0000269|PubMed:11266443}.
CC -!- INTERACTION:
CC P40960; P36022: DYN1; NbExp=3; IntAct=EBI-30551, EBI-6230;
CC P40960; Q02647: DYN2; NbExp=4; IntAct=EBI-30551, EBI-6240;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 752 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U16820; AAB00684.1; -; Genomic_DNA.
DR EMBL; U33050; AAB64926.1; -; Genomic_DNA.
DR EMBL; AY723795; AAU09712.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12321.1; -; Genomic_DNA.
DR PIR; S69655; S69655.
DR RefSeq; NP_010776.1; NM_001180796.1.
DR PDB; 4HT6; X-ray; 1.90 A; B/D/F=75-85.
DR PDBsum; 4HT6; -.
DR AlphaFoldDB; P40960; -.
DR SMR; P40960; -.
DR BioGRID; 32540; 280.
DR ComplexPortal; CPX-1178; Cytoplasmic dynein complex.
DR DIP; DIP-5268N; -.
DR IntAct; P40960; 21.
DR MINT; P40960; -.
DR STRING; 4932.YDR488C; -.
DR MaxQB; P40960; -.
DR PaxDb; 4932-YDR488C; -.
DR PeptideAtlas; P40960; -.
DR EnsemblFungi; YDR488C_mRNA; YDR488C; YDR488C.
DR GeneID; 852099; -.
DR KEGG; sce:YDR488C; -.
DR AGR; SGD:S000002896; -.
DR SGD; S000002896; PAC11.
DR VEuPathDB; FungiDB:YDR488C; -.
DR eggNOG; ENOG502R5J2; Eukaryota.
DR HOGENOM; CLU_020687_0_0_1; -.
DR InParanoid; P40960; -.
DR OMA; NIMELSC; -.
DR OrthoDB; 2024203at2759; -.
DR BioCyc; YEAST:G3O-30012-MONOMER; -.
DR BioGRID-ORCS; 852099; 5 hits in 10 CRISPR screens.
DR PRO; PR:P40960; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; P40960; Protein.
DR GO; GO:0005737; C:cytoplasm; NAS:ComplexPortal.
DR GO; GO:0005868; C:cytoplasmic dynein complex; IPI:SGD.
DR GO; GO:0005881; C:cytoplasmic microtubule; IDA:SGD.
DR GO; GO:0045504; F:dynein heavy chain binding; IBA:GO_Central.
DR GO; GO:0045503; F:dynein light chain binding; IBA:GO_Central.
DR GO; GO:0040001; P:establishment of mitotic spindle localization; IMP:SGD.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; NAS:ComplexPortal.
DR GO; GO:0030473; P:nuclear migration along microtubule; IMP:SGD.
DR GO; GO:0010970; P:transport along microtubule; IBA:GO_Central.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR12442:SF26; CYTOPLASMIC DYNEIN 2 INTERMEDIATE CHAIN 2; 1.
DR PANTHER; PTHR12442; DYNEIN INTERMEDIATE CHAIN; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Cytoskeleton; Microtubule; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..533
FT /note="WD repeat-containing protein PAC11"
FT /id="PRO_0000051114"
FT REPEAT 380..422
FT /note="WD 1"
FT REPEAT 432..475
FT /note="WD 2"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..25
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 140
FT /note="L -> V (in Ref. 1; AAB00684)"
FT /evidence="ECO:0000305"
FT STRAND 76..82
FT /evidence="ECO:0007829|PDB:4HT6"
SQ SEQUENCE 533 AA; 60074 MW; 2037021799414E9A CRC64;
MERLKQLEEK RRQLKELRER RKQASLFPGS ETMGHHPTEV HAKATMVSVS VQTDMEEGSK
IQEPQSAYLR RKEVITYDKG IQTDQIEEEQ LQENENHTTT DAVAIETTAA DENNKDKAEN
DQPRLELAKP FLVEEAAATL SNASFARLET EVSASGQQAP SNMQQDKDNL MQWNMVSENL
QSETDCDCIA QEYDPGKGVL VVVYLRLPPA DLQYASSEAA WSVVNVVKCD NASGRNGLLI
DMVEFRGTRI MTATILRRYH PESNVISILL ATLTGKIILY ELRLKQKKPE TPVVYVVQRN
MVARHYFQHP VVAVIETSSV QDQERVLVAA DNGNIMELSC LDLTVLRKPQ QLRPVPLSQL
LSLENDTCTY TERLQRLAKF DEVGIACMAY TSEDPQYVWI GGEDGGIYKV FWDQPGPLYL
SLDNNGFQPA ENHSTRVTGL EFHWDDARRL MLLLSCSTDW TVRLWDARAG KAIIGAPLLL
GGPVLRARWL EKNNGGENSR TLRCQVWCAD GRLVVVNWAF DAKTSLYTAT VIS
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