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Database: UniProt
Entry: P44647
LinkDB: P44647
Original site: P44647 
ID   PRIA_HAEIN              Reviewed;         730 AA.
AC   P44647;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   16-OCT-2019, entry version 123.
DE   RecName: Full=Primosomal protein N' {ECO:0000255|HAMAP-Rule:MF_00983};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_00983};
DE   AltName: Full=ATP-dependent helicase PriA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   Name=priA {ECO:0000255|HAMAP-Rule:MF_00983};
GN   OrderedLocusNames=HI_0339;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA   Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA   Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA   Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA   Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA   Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Involved in the restart of stalled replication forks.
CC       Recognizes and binds the arrested nascent DNA chain at stalled
CC       replication forks. It can open the DNA duplex, via its helicase
CC       activity, and promote assembly of the primosome and loading of the
CC       major replicative helicase DnaB onto DNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SUBUNIT: Component of the primosome. {ECO:0000255|HAMAP-
CC       Rule:MF_00983}.
CC   -!- SIMILARITY: Belongs to the helicase family. PriA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00983}.
DR   EMBL; L42023; AAC22001.1; -; Genomic_DNA.
DR   PIR; G64062; G64062.
DR   RefSeq; NP_438503.1; NC_000907.1.
DR   RefSeq; WP_005694336.1; NC_000907.1.
DR   SMR; P44647; -.
DR   STRING; 71421.HI_0339; -.
DR   PRIDE; P44647; -.
DR   EnsemblBacteria; AAC22001; AAC22001; HI_0339.
DR   GeneID; 950761; -.
DR   KEGG; hin:HI0339; -.
DR   PATRIC; fig|71421.8.peg.356; -.
DR   eggNOG; ENOG4105C25; Bacteria.
DR   eggNOG; COG1198; LUCA.
DR   KO; K04066; -.
DR   OMA; RCHYCGY; -.
DR   PhylomeDB; P44647; -.
DR   BioCyc; HINF71421:G1GJ1-355-MONOMER; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR   GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR   GO; GO:0006302; P:double-strand break repair; IBA:GO_Central.
DR   Gene3D; 1.10.8.630; -; 1.
DR   HAMAP; MF_00983; PriA; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005259; PriA.
DR   InterPro; IPR041222; PriA_3primeBD.
DR   InterPro; IPR042115; PriA_3primeBD_sf.
DR   InterPro; IPR041236; PriA_C.
DR   InterPro; IPR040498; PriA_CRR.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF17764; PriA_3primeBD; 1.
DR   Pfam; PF18074; PriA_C; 1.
DR   Pfam; PF18319; PriA_CRR; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00595; priA; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA replication; DNA-binding;
KW   Helicase; Hydrolase; Metal-binding; Nucleotide-binding; Primosome;
KW   Reference proteome; Zinc; Zinc-finger.
FT   CHAIN         1    730       Primosomal protein N'.
FT                                /FTId=PRO_0000102124.
FT   DOMAIN      212    378       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   DOMAIN      472    640       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   NP_BIND     225    232       ATP. {ECO:0000255|HAMAP-Rule:MF_00983}.
FT   ZN_FING     437    449       C4-type. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   ZN_FING     464    480       C4-type. {ECO:0000255|HAMAP-
FT                                Rule:MF_00983}.
FT   MOTIF       321    324       DEAH box.
SQ   SEQUENCE   730 AA;  82336 MW;  5DB1D638426C4714 CRC64;
     MKIVRVALAV PLPRLFDYFV PDDVSLQIGM RVLVPFGTQK RVAIVADFPT KSDVPEDKLK
     AILQPLDLAP LFTPIYWDWL HWAANYYQAG LGDVLFQALP VKLRNGESAV KNDRTFWRIT
     DAGKNALKQG ELKRSKKQAE ALQYLSETDL EKGNNDFSSA IWSALKAKGF IEEITIQTNP
     LSWQQRLGNN PIVNAENRLT LNKQQALAFS QLLFHSGFNV WLLDGVTGSG KTEIYLQYIE
     EILKSGKQVL VLVPEIGLTP QTVQRFKVRF NVEIDVLHSN LTDTQRLYVW DRARSGQSAI
     VIGTRSALFT QFSNLGAIIL DEEHDSSYKQ QDSWRYHARD LAIVLAQKLN ISVLMGSATP
     SLESINNVQN GKYQHLVLSK RAGNSTALRH FVIDLKNQNI QNGLSKPLLE RMKAHLEKGN
     QVLLFLNRRG FAPVLLCHEC GWIAQCPHCE KPYTYHQHQN VLRCHHCGAQ KTIPRQCGDC
     GSTHLVTTGL GTEQLEETLK TLFPHYSVAR IDRDSTSRKG KLEGYLEDIQ QGKSQILIGT
     QMLAKGHHFP NVTLVALVNV DSALFSLDFR AEERLAQLYI QVAGRAGRAD KQGEVVLQTH
     YPDHPLLTTL LANGYQAFAK ETLQLRHSMG LPPFTFQALI KAQARHSDLA ENCLSQIADF
     FQSKQITGLQ MLGPMPAPFS KKAGQYRWQL LLQHPSRMTL QKALREYQQA ELEKNSQVRL
     ILDVDPQDLS
//
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