GenomeNet

Database: UniProt
Entry: P44781
LinkDB: P44781
Original site: P44781 
ID   RAPA_HAEIN              Reviewed;         923 AA.
AC   P44781;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   31-JUL-2019, entry version 116.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821}; Synonyms=hepA;
GN   OrderedLocusNames=HI_0616;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA   Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA   Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA   Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA   Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA   Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; L42023; AAC22275.1; -; Genomic_DNA.
DR   PIR; H64081; H64081.
DR   RefSeq; NP_438774.1; NC_000907.1.
DR   RefSeq; WP_005692755.1; NC_000907.1.
DR   STRING; 71421.HI_0616; -.
DR   PRIDE; P44781; -.
DR   EnsemblBacteria; AAC22275; AAC22275; HI_0616.
DR   GeneID; 949665; -.
DR   KEGG; hin:HI0616; -.
DR   PATRIC; fig|71421.8.peg.640; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   PhylomeDB; P44781; -.
DR   BioCyc; HINF71421:G1GJ1-637-MONOMER; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    923       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_0000207177.
FT   DOMAIN      162    332       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      443    597       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     175    182       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       278    281       DEAH box.
SQ   SEQUENCE   923 AA;  104405 MW;  8022403581DAADBD CRC64;
     MPFAIGQRWL SESENALGLG VITALDQRTV TIYFPAADET RIYAMAQAPL SRIVFSKGET
     LSHQAGWQGE ILDVQNMNGL LFYLVKNPQD EDVIVQERDI SPIISFSQAK DRLFSAQIDR
     STHFALRYRT LCHQQAQFKS PLRGLRGTRA SLIPHQLHIA AEVGNRVNPR VLLADEVGLG
     KTIEAGMILQ NQLFAEKVQR VLIIVPETLQ HQWLVEMLRR FNLHFALFDE ERCNDFDLDA
     VNPFTTESLI ICSLNWLETH PNRVELVLNA QFDCLIVDEA HHLVWSETSP STAYLLVEQL
     ARIIPSVLLL TATPEQLGQE SHFARLRLLD PERFFDYQTF VKEQEHYQPV VNAVESLLAN
     KALSAVEKNH ISDLLLEQDV EPLFKAIASN NDEEQQRARQ ELIQALIDRH GTGRMLFRNT
     RQGVKGFPHR VYHQITLSEE NDKIDWLIDF LKLHRNEKIF VICQTAATAI QLEQILRERE
     AIRAAVFHEK MSIIERDRAA AYFADLENGA QVLLSSSIGS EGRNFQFAAN LVLFDLPTNP
     DLLEQCIGRL DRIGQKRDVQ VYVPCAKDSP QSRLARWYNE GLNAFEQTCP MGMALFSQFA
     DELEKVRSNS TALSENEFSG LLKQTKTARE KLKIELEKGR DRLLELNSHG GEQAQALADQ
     IADEDNSPEL VNFALKLFDI IGVEQEDLGA NSIVISPTGT MLVPDFPGLK EEGVTVTFDR
     ELALAREEME FLTWDHPMIR QGIDLVASGD IGKAAMALLV NKQLPAGTLL IELIYVVESQ
     SPKGLQLNRF LPPTPIRLLL DNKGNNIGEQ VAFETLHSKL KPLGKNITNQ MVKMARSNIE
     SLIMRGDQLV KSLAEPIIAE AKNQADQQLS AEINRLQALR AVNKNIRQSE IDILEQQRTQ
     SLDELSKANW RLDCLRVIVT NKE
//
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