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Database: UniProt
Entry: P47047
LinkDB: P47047
Original site: P47047 
ID   MTR4_YEAST              Reviewed;        1073 AA.
AC   P47047; D6VWD3;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   16-OCT-2019, entry version 180.
DE   RecName: Full=ATP-dependent RNA helicase DOB1;
DE            EC=3.6.4.13;
DE   AltName: Full=mRNA transport regulator MTR4;
GN   Name=MTR4; Synonyms=DOB1; OrderedLocusNames=YJL050W; ORFNames=J1158;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N.,
RA   Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H.,
RA   Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A.,
RA   Hennemann A., Herbert C.J., Heumann K., Hilger F., Hollenberg C.P.,
RA   Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L.,
RA   Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V.,
RA   Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M.,
RA   Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W.,
RA   Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M.,
RA   Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A.,
RA   Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M.,
RA   Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=8756671; DOI=10.1128/mcb.16.9.5139;
RA   Liang S., Hitomi M., Hu Y.-H., Liu Y., Tartakoff A.M.;
RT   "A DEAD-box-family protein is required for nucleocytoplasmic transport
RT   of yeast mRNA.";
RL   Mol. Cell. Biol. 16:5139-5146(1996).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=9463390; DOI=10.1093/emboj/17.4.1128;
RA   de la Cruz J., Kressler D., Tollervey D., Linder P.;
RT   "Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for
RT   the 3' end formation of 5.8S rRNA in Saccharomyces cerevisiae.";
RL   EMBO J. 17:1128-1140(1998).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   IDENTIFICATION IN THE TRF4 COMPLEX, IDENTIFICATION BY MASS
RP   SPECTROMETRY, AND FUNCTION OF THE TRF4 COMPLEX.
RX   PubMed=15828860; DOI=10.1371/journal.pbio.0030189;
RA   Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A.,
RA   Langen H., Keith G., Keller W.;
RT   "A new yeast poly(A) polymerase complex involved in RNA quality
RT   control.";
RL   PLoS Biol. 3:986-997(2005).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-34, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA   Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA   Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT   "Analysis of phosphorylation sites on proteins from Saccharomyces
RT   cerevisiae by electron transfer dissociation (ETD) mass
RT   spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84 AND SER-843, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides
RT   insights into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: ATP-dependent RNA helicase required for the 3'-end
CC       formation of 5.8S RNA. Cofactor for the exosome complex that
CC       unwinds secondary structure in pre-rRNA. Required for
CC       nucleocytoplasmic transport of mRNA. May serve as a chaperone
CC       which translocates or normalizes the structure of mRNAs in
CC       preparation for export. Component of the TRAMP complex which has a
CC       poly(A) RNA polymerase activity and is involved in a post-
CC       transcriptional quality control mechanism limiting inappropriate
CC       expression of genetic information. Polyadenylation is required for
CC       the degradative activity of the exosome on several of its nuclear
CC       RNA substrates. {ECO:0000269|PubMed:15828860}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SUBUNIT: Component of the TRAMP complex (also called TRF4 complex)
CC       composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or
CC       AIR2. {ECO:0000269|PubMed:15828860}.
CC   -!- INTERACTION:
CC       Q12476:AIR2; NbExp=7; IntAct=EBI-11592, EBI-31475;
CC       P38074:HMT1; NbExp=2; IntAct=EBI-11592, EBI-8394;
CC       P53632:PAP2; NbExp=11; IntAct=EBI-11592, EBI-19517;
CC       P48561:TRF5; NbExp=6; IntAct=EBI-11592, EBI-19525;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- MISCELLANEOUS: Present with 12500 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the helicase family. SKI2 subfamily.
CC       {ECO:0000305}.
DR   EMBL; Z49325; CAA89341.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08749.1; -; Genomic_DNA.
DR   PIR; S56822; S56822.
DR   RefSeq; NP_012485.1; NM_001181483.1.
DR   PDB; 2XGJ; X-ray; 2.90 A; A/B=81-1073.
DR   PDB; 4QU4; X-ray; 3.39 A; A=1-1073.
DR   PDB; 4U4C; X-ray; 2.40 A; A=81-1073.
DR   PDB; 4WFD; X-ray; 2.40 A; C/F/I=1-19.
DR   PDB; 5OOQ; X-ray; 3.20 A; A/B=81-1073.
DR   PDB; 6FSZ; EM; 4.60 A; MM=1-1073.
DR   PDB; 6FT6; EM; 3.90 A; MM=80-1073.
DR   PDBsum; 2XGJ; -.
DR   PDBsum; 4QU4; -.
DR   PDBsum; 4U4C; -.
DR   PDBsum; 4WFD; -.
DR   PDBsum; 5OOQ; -.
DR   PDBsum; 6FSZ; -.
DR   PDBsum; 6FT6; -.
DR   SMR; P47047; -.
DR   BioGrid; 33705; 270.
DR   ComplexPortal; CPX-1678; TRAMP complex variant 1.
DR   ComplexPortal; CPX-1679; TRAMP complex variant 2.
DR   ComplexPortal; CPX-1680; TRAMP5 complex.
DR   DIP; DIP-6394N; -.
DR   ELM; P47047; -.
DR   IntAct; P47047; 19.
DR   MINT; P47047; -.
DR   STRING; 4932.YJL050W; -.
DR   iPTMnet; P47047; -.
DR   MaxQB; P47047; -.
DR   PaxDb; P47047; -.
DR   PRIDE; P47047; -.
DR   EnsemblFungi; YJL050W_mRNA; YJL050W; YJL050W.
DR   GeneID; 853397; -.
DR   KEGG; sce:YJL050W; -.
DR   EuPathDB; FungiDB:YJL050W; -.
DR   SGD; S000003586; MTR4.
DR   HOGENOM; HOG000163047; -.
DR   InParanoid; P47047; -.
DR   KO; K12598; -.
DR   OMA; CFVCDEK; -.
DR   BioCyc; YEAST:G3O-31514-MONOMER; -.
DR   BRENDA; 3.6.4.13; 984.
DR   Reactome; R-SCE-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   EvolutionaryTrace; P47047; -.
DR   PRO; PR:P47047; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0031499; C:TRAMP complex; IDA:SGD.
DR   GO; GO:0034458; F:3'-5' RNA helicase activity; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003729; F:mRNA binding; HDA:SGD.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0008143; F:poly(A) binding; IDA:SGD.
DR   GO; GO:0000467; P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
DR   GO; GO:0000460; P:maturation of 5.8S rRNA; IBA:GO_Central.
DR   GO; GO:0006397; P:mRNA processing; IMP:SGD.
DR   GO; GO:0043629; P:ncRNA polyadenylation; IDA:SGD.
DR   GO; GO:0071031; P:nuclear mRNA surveillance of mRNA 3'-end processing; IMP:SGD.
DR   GO; GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IMP:SGD.
DR   GO; GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:SGD.
DR   GO; GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IDA:SGD.
DR   GO; GO:0071049; P:nuclear retention of pre-mRNA with aberrant 3'-ends at the site of transcription; IGI:SGD.
DR   GO; GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IMP:SGD.
DR   GO; GO:0006401; P:RNA catabolic process; IBA:GO_Central.
DR   GO; GO:0016075; P:rRNA catabolic process; IMP:SGD.
DR   GO; GO:0034475; P:U4 snRNA 3'-end processing; IMP:SGD.
DR   GO; GO:0034476; P:U5 snRNA 3'-end processing; IMP:SGD.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR011254; Prismane-like_sf.
DR   InterPro; IPR025696; rRNA_proc-arch_dom.
DR   InterPro; IPR016438; Ski2-like.
DR   InterPro; IPR012961; Ski2_C.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF08148; DSHCT; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF13234; rRNA_proc-arch; 1.
DR   PIRSF; PIRSF005198; Antiviral_helicase_SKI2; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM01142; DSHCT; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF56821; SSF56821; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome;
KW   rRNA processing.
FT   CHAIN         1   1073       ATP-dependent RNA helicase DOB1.
FT                                /FTId=PRO_0000102092.
FT   DOMAIN      158    314       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      393    597       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     171    178       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       262    265       DEVH box.
FT   MOD_RES      34     34       Phosphothreonine.
FT                                {ECO:0000244|PubMed:17287358}.
FT   MOD_RES      84     84       Phosphoserine.
FT                                {ECO:0000244|PubMed:18407956,
FT                                ECO:0000244|PubMed:19779198}.
FT   MOD_RES     843    843       Phosphoserine.
FT                                {ECO:0000244|PubMed:18407956}.
FT   HELIX         8     11       {ECO:0000244|PDB:4WFD}.
FT   STRAND       81     94       {ECO:0000244|PDB:2XGJ}.
FT   STRAND      100    102       {ECO:0000244|PDB:2XGJ}.
FT   STRAND      119    124       {ECO:0000244|PDB:4U4C}.
FT   HELIX       134    136       {ECO:0000244|PDB:4U4C}.
FT   HELIX       152    162       {ECO:0000244|PDB:4U4C}.
FT   STRAND      166    170       {ECO:0000244|PDB:4U4C}.
FT   HELIX       173    175       {ECO:0000244|PDB:4U4C}.
FT   HELIX       177    190       {ECO:0000244|PDB:4U4C}.
FT   STRAND      194    201       {ECO:0000244|PDB:4U4C}.
FT   HELIX       202    216       {ECO:0000244|PDB:4U4C}.
FT   STRAND      219    222       {ECO:0000244|PDB:4U4C}.
FT   STRAND      233    238       {ECO:0000244|PDB:4U4C}.
FT   HELIX       239    247       {ECO:0000244|PDB:4U4C}.
FT   HELIX       252    255       {ECO:0000244|PDB:4U4C}.
FT   STRAND      256    261       {ECO:0000244|PDB:4U4C}.
FT   HELIX       264    268       {ECO:0000244|PDB:4U4C}.
FT   TURN        270    272       {ECO:0000244|PDB:4U4C}.
FT   HELIX       273    282       {ECO:0000244|PDB:4U4C}.
FT   STRAND      288    293       {ECO:0000244|PDB:4U4C}.
FT   HELIX       299    310       {ECO:0000244|PDB:4U4C}.
FT   STRAND      314    319       {ECO:0000244|PDB:4U4C}.
FT   STRAND      326    332       {ECO:0000244|PDB:4U4C}.
FT   STRAND      336    342       {ECO:0000244|PDB:4U4C}.
FT   HELIX       350    361       {ECO:0000244|PDB:4U4C}.
FT   HELIX       393    403       {ECO:0000244|PDB:4U4C}.
FT   STRAND      408    412       {ECO:0000244|PDB:4U4C}.
FT   STRAND      413    415       {ECO:0000244|PDB:4QU4}.
FT   HELIX       416    426       {ECO:0000244|PDB:4U4C}.
FT   STRAND      427    429       {ECO:0000244|PDB:4QU4}.
FT   HELIX       434    448       {ECO:0000244|PDB:4U4C}.
FT   HELIX       453    456       {ECO:0000244|PDB:4U4C}.
FT   HELIX       459    462       {ECO:0000244|PDB:4U4C}.
FT   HELIX       465    469       {ECO:0000244|PDB:4U4C}.
FT   STRAND      472    475       {ECO:0000244|PDB:4U4C}.
FT   STRAND      477    479       {ECO:0000244|PDB:4QU4}.
FT   HELIX       481    492       {ECO:0000244|PDB:4U4C}.
FT   STRAND      497    501       {ECO:0000244|PDB:4U4C}.
FT   HELIX       503    507       {ECO:0000244|PDB:4U4C}.
FT   STRAND      513    519       {ECO:0000244|PDB:4U4C}.
FT   STRAND      521    524       {ECO:0000244|PDB:4U4C}.
FT   STRAND      526    531       {ECO:0000244|PDB:4U4C}.
FT   HELIX       534    541       {ECO:0000244|PDB:4U4C}.
FT   TURN        547    549       {ECO:0000244|PDB:4U4C}.
FT   STRAND      553    559       {ECO:0000244|PDB:4U4C}.
FT   HELIX       565    572       {ECO:0000244|PDB:4U4C}.
FT   HELIX       587    595       {ECO:0000244|PDB:4U4C}.
FT   STRAND      596    598       {ECO:0000244|PDB:2XGJ}.
FT   HELIX       601    607       {ECO:0000244|PDB:4U4C}.
FT   HELIX       609    635       {ECO:0000244|PDB:4U4C}.
FT   HELIX       642    664       {ECO:0000244|PDB:4U4C}.
FT   HELIX       667    670       {ECO:0000244|PDB:4U4C}.
FT   HELIX       671    673       {ECO:0000244|PDB:4U4C}.
FT   STRAND      678    684       {ECO:0000244|PDB:4U4C}.
FT   TURN        685    687       {ECO:0000244|PDB:4U4C}.
FT   STRAND      688    700       {ECO:0000244|PDB:4U4C}.
FT   STRAND      704    708       {ECO:0000244|PDB:5OOQ}.
FT   HELIX       713    715       {ECO:0000244|PDB:4U4C}.
FT   STRAND      716    727       {ECO:0000244|PDB:4U4C}.
FT   HELIX       732    734       {ECO:0000244|PDB:4U4C}.
FT   STRAND      755    763       {ECO:0000244|PDB:4U4C}.
FT   HELIX       764    766       {ECO:0000244|PDB:4U4C}.
FT   STRAND      767    774       {ECO:0000244|PDB:4U4C}.
FT   HELIX       786    800       {ECO:0000244|PDB:4U4C}.
FT   STRAND      801    803       {ECO:0000244|PDB:2XGJ}.
FT   TURN        810    814       {ECO:0000244|PDB:4U4C}.
FT   HELIX       819    837       {ECO:0000244|PDB:4U4C}.
FT   HELIX       839    841       {ECO:0000244|PDB:4U4C}.
FT   STRAND      843    845       {ECO:0000244|PDB:2XGJ}.
FT   HELIX       847    871       {ECO:0000244|PDB:4U4C}.
FT   HELIX       876    891       {ECO:0000244|PDB:4U4C}.
FT   HELIX       903    909       {ECO:0000244|PDB:4U4C}.
FT   HELIX       916    925       {ECO:0000244|PDB:4U4C}.
FT   TURN        926    930       {ECO:0000244|PDB:4U4C}.
FT   HELIX       933    944       {ECO:0000244|PDB:4U4C}.
FT   STRAND      950    952       {ECO:0000244|PDB:4U4C}.
FT   HELIX       957    979       {ECO:0000244|PDB:4U4C}.
FT   HELIX       986    992       {ECO:0000244|PDB:4U4C}.
FT   HELIX       998   1006       {ECO:0000244|PDB:4U4C}.
FT   HELIX      1010   1015       {ECO:0000244|PDB:4U4C}.
FT   HELIX      1021   1043       {ECO:0000244|PDB:4U4C}.
FT   TURN       1044   1046       {ECO:0000244|PDB:4U4C}.
FT   HELIX      1048   1061       {ECO:0000244|PDB:4U4C}.
FT   HELIX      1064   1067       {ECO:0000244|PDB:4U4C}.
SQ   SEQUENCE   1073 AA;  122055 MW;  1FA9EAADC546F669 CRC64;
     MDSTDLFDVF EETPVELPTD SNGEKNADTN VGDTPDHTQD KKHGLEEEKE EHEENNSENK
     KIKSNKSKTE DKNKKVVVPV LADSFEQEAS REVDASKGLT NSETLQVEQD GKVRLSHQVR
     HQVALPPNYD YTPIAEHKRV NEARTYPFTL DPFQDTAISC IDRGESVLVS AHTSAGKTVV
     AEYAIAQSLK NKQRVIYTSP IKALSNQKYR ELLAEFGDVG LMTGDITINP DAGCLVMTTE
     ILRSMLYRGS EVMREVAWVI FDEVHYMRDK ERGVVWEETI ILLPDKVRYV FLSATIPNAM
     EFAEWICKIH SQPCHIVYTN FRPTPLQHYL FPAHGDGIYL VVDEKSTFRE ENFQKAMASI
     SNQIGDDPNS TDSRGKKGQT YKGGSAKGDA KGDIYKIVKM IWKKKYNPVI VFSFSKRDCE
     ELALKMSKLD FNSDDEKEAL TKIFNNAIAL LPETDRELPQ IKHILPLLRR GIGIHHSGLL
     PILKEVIEIL FQEGFLKVLF ATETFSIGLN MPAKTVVFTS VRKWDGQQFR WVSGGEYIQM
     SGRAGRRGLD DRGIVIMMID EKMEPQVAKG MVKGQADRLD SAFHLGYNMI LNLMRVEGIS
     PEFMLEHSFF QFQNVISVPV MEKKLAELKK DFDGIEVEDE ENVKEYHEIE QAIKGYREDV
     RQVVTHPANA LSFLQPGRLV EISVNGKDNY GWGAVVDFAK RINKRNPSAV YTDHESYIVN
     VVVNTMYIDS PVNLLKPFNP TLPEGIRPAE EGEKSICAVI PITLDSIKSI GNLRLYMPKD
     IRASGQKETV GKSLREVNRR FPDGIPVLDP VKNMKIEDED FLKLMKKIDV LNTKLSSNPL
     TNSMRLEELY GKYSRKHDLH EDMKQLKRKI SESQAVIQLD DLRRRKRVLR RLGFCTPNDI
     IELKGRVACE ISSGDELLLT ELIFNGNFNE LKPEQAAALL SCFAFQERCK EAPRLKPELA
     EPLKAMREIA AKIAKIMKDS KIEVVEKDYV ESFRHELMEV VYEWCRGATF TQICKMTDVY
     EGSLIRMFKR LEELVKELVD VANTIGNSSL KEKMEAVLKL IHRDIVSAGS LYL
//
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