ID SYR_MYCGE Reviewed; 537 AA.
AC P47618; Q49291; Q49444;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 27-MAR-2024, entry version 144.
DE RecName: Full=Arginine--tRNA ligase;
DE EC=6.1.1.19;
DE AltName: Full=Arginyl-tRNA synthetase;
DE Short=ArgRS;
GN Name=argS; OrderedLocusNames=MG378;
OS Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
OS (Mycoplasmoides genitalium).
OC Bacteria; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae;
OC Mycoplasmoides.
OX NCBI_TaxID=243273;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT "The minimal gene complement of Mycoplasma genitalium.";
RL Science 270:397-403(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 153-255 AND 350-455.
RC STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX PubMed=8253680; DOI=10.1128/jb.175.24.7918-7930.1993;
RA Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III;
RT "A survey of the Mycoplasma genitalium genome by using random sequencing.";
RL J. Bacteriol. 175:7918-7930(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; L43967; AAC71605.1; -; Genomic_DNA.
DR EMBL; U02168; AAD12450.1; -; Genomic_DNA.
DR EMBL; U01740; AAD10550.1; -; Genomic_DNA.
DR PIR; H64241; H64241.
DR RefSeq; WP_009885941.1; NZ_AAGX01000012.1.
DR AlphaFoldDB; P47618; -.
DR SMR; P47618; -.
DR STRING; 243273.MG_378; -.
DR KEGG; mge:MG_378; -.
DR eggNOG; COG0018; Bacteria.
DR HOGENOM; CLU_006406_0_1_14; -.
DR InParanoid; P47618; -.
DR OrthoDB; 9805987at2; -.
DR BioCyc; MGEN243273:G1GJ2-472-MONOMER; -.
DR Proteomes; UP000000807; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00456; argS; 1.
DR PANTHER; PTHR11956:SF5; ARGININE--TRNA LIGASE, CYTOPLASMIC-RELATED; 1.
DR PANTHER; PTHR11956; ARGINYL-TRNA SYNTHETASE; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..537
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151576"
FT MOTIF 113..123
FT /note="'HIGH' region"
FT CONFLICT 153..154
FT /note="YG -> MW (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 350..355
FT /note="FALQLV -> MHYNWI (in Ref. 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 537 AA; 62406 MW; D8080D69EDA33817 CRC64;
MFFIINDLKE CISALKLKFD DQKELVKLVK NNSFNGFSST IIFQLKSENH KKIADSIVEW
FLKNKKDNYQ NVFIANNNFI NFQISYQKYL EYLIKTPCFT KKNIKILIES VSANPTGRIH
LGHVRIAFFG DVLNNLAKLL GYTTVCEYWV NDYGQQARVF SFSVYQSLQL KKNIAIQQHP
DGYSGIVIDK IASEIENFPV DNLNFEEFCK TSFLDHFLVN CTQKVLSLIK SDLNKIHVFI
DSWKFESEIV KKTNFNDLLE QLKPNSYFYQ DNALWLKTTL YGDDKDRVLI RSDKRASYFG
TDVAYHLEKL QRGFDILFNV WGTDHEGHIK RMYCAFDALK NTTKTSLKIF ALQLVTLYKN
KELVRLSKRA GNVITIETML SMISEDAARW FMLSQNNGTI IKIDLDIANL QNSANPVYYV
QYAFARMNSI LRIANSDQLK EITDCSLLIN EKEISLLNQL VYYPFMLQKA METGELHLLT
NFLYETASLF HSWYKVCKIN DDKNSLLSAQ RLALLRSLQF IVKQILDVLK ISTPQQM
//
