GenomeNet

Database: UniProt
Entry: P51011
LinkDB: P51011
Original site: P51011 
ID   LDHD_LEUMC              Reviewed;         331 AA.
AC   P51011;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   05-DEC-2018, entry version 87.
DE   RecName: Full=D-lactate dehydrogenase;
DE            Short=D-LDH;
DE            EC=1.1.1.28;
DE   AltName: Full=D-specific 2-hydroxyacid dehydrogenase;
OS   Leuconostoc mesenteroides subsp. cremoris.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Leuconostocaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=33965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=195;
RX   PubMed=7569323; DOI=10.1016/0923-2508(96)81052-7;
RA   Dartois V., Phalip V., Schmitt P., Divies C.;
RT   "Purification, properties and DNA sequence of the D-lactate
RT   dehydrogenase from Leuconostoc mesenteroides subsp. cremoris.";
RL   Res. Microbiol. 146:291-302(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(R)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC         Xref=Rhea:RHEA:16369, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16004, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         EC=1.1.1.28;
CC   -!- SUBUNIT: Homodimer.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. {ECO:0000305}.
DR   EMBL; L29327; AAA99506.1; -; Genomic_DNA.
DR   ProteinModelPortal; P51011; -.
DR   SMR; P51011; -.
DR   BioCyc; MetaCyc:MONOMER-13061; -.
DR   BRENDA; 1.1.1.28; 839.
DR   GO; GO:0008720; F:D-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR   InterPro; IPR029753; D-isomer_DH_CS.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR006140; D-isomer_DH_NAD-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00671; D_2_HYDROXYACID_DH_3; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    331       D-lactate dehydrogenase.
FT                                /FTId=PRO_0000075957.
FT   NP_BIND     155    156       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   NP_BIND     206    207       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   NP_BIND     233    235       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   ACT_SITE    235    235       {ECO:0000250|UniProtKB:P26297}.
FT   ACT_SITE    264    264       {ECO:0000250|UniProtKB:P26297}.
FT   ACT_SITE    296    296       Proton donor.
FT                                {ECO:0000250|UniProtKB:P26297}.
FT   BINDING     175    175       NAD. {ECO:0000250|UniProtKB:P26297}.
FT   BINDING     212    212       NAD. {ECO:0000250|UniProtKB:P30901}.
FT   BINDING     259    259       NAD. {ECO:0000250|UniProtKB:P30901}.
SQ   SEQUENCE   331 AA;  36316 MW;  0A63C462303672D8 CRC64;
     MKIFAYGIRD DEKPSLEEWK AANPEIEVDY TQELLTPETA KLAEGSDSAV VYQQLDYTRE
     TLTALANVGV TNLSLRNVGT DNIDFDAARE FNFNISNVPV YSPNAIAEHS MLQLSRLLRR
     TKALDAKIAK RDLRWAPTTG REMRMQTVGV IGTGHIGRVA INILKGFGAK VIAYDKYPNA
     ELQAEGLYVD TLDELYAQAD AISLYVPGVP ENHHLINADA IAKMKDGVVI MNAARGNLMD
     IDAIIDGLNS GKISDFGMDV YENEVACSMK IGLVKNSPDA KIADLIAREN VMITPHTAFY
     TTKAVLEMVH QSFDAAVAFA KGEKPAIAVE Y
//
DBGET integrated database retrieval system