ID CITB_KLEPN Reviewed; 234 AA.
AC P52688;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 24-JAN-2024, entry version 117.
DE RecName: Full=Transcriptional regulatory protein CitB;
GN Name=citB;
OS Klebsiella pneumoniae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13882 / NBRC 13541 / NCTC 8172;
RX PubMed=8748036; DOI=10.1111/j.1365-2958.1995.mmi_18030533.x;
RA Bott M., Meyer M., Dimroth P.;
RT "Regulation of anaerobic citrate metabolism in Klebsiella pneumoniae.";
RL Mol. Microbiol. 18:533-546(1995).
RN [2]
RP PROTEIN SEQUENCE OF 1-6, AND CHARACTERIZATION.
RC STRAIN=ATCC 13882 / NBRC 13541 / NCTC 8172;
RX PubMed=9223636; DOI=10.1006/jmbi.1997.1076;
RA Meyer M., Dimroth P., Bott M.;
RT "In vitro binding of the response regulator CitB and of its carboxy-
RT terminal domain to A + T-rich DNA target sequences in the control region of
RT the divergent citC and citS operons of Klebsiella pneumoniae.";
RL J. Mol. Biol. 269:719-731(1997).
RN [3]
RP FUNCTION, PHOSPHORYLATION AT ASP-56, CHARACTERIZATION, AND MUTAGENESIS OF
RP ASP-56.
RC STRAIN=ATCC 13882 / NBRC 13541 / NCTC 8172;
RX PubMed=10447894; DOI=10.1046/j.1365-2958.1999.01536.x;
RA Kaspar S., Perozzo R., Reinelt S., Meyer M., Pfister K., Scapozza L.,
RA Bott M.;
RT "The periplasmic domain of the histidine autokinase CitA functions as a
RT highly specific citrate receptor.";
RL Mol. Microbiol. 33:858-872(1999).
CC -!- FUNCTION: Member of the two-component regulatory system CitA/CitB
CC essential for expression of citrate-specific fermentation genes.
CC Phosphorylated CitB binds to two sites in the citS-citC intergenic
CC region where it probably activates transcription of both genes.
CC {ECO:0000269|PubMed:10447894}.
CC -!- SUBUNIT: In vitro CitB and the CitA kinase domain form a complex,
CC formation of which is enhanced by ATP.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by CitA. {ECO:0000269|PubMed:10447894}.
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DR EMBL; U31464; AAC44734.1; -; Genomic_DNA.
DR PIR; S70539; S70539.
DR RefSeq; WP_004222618.1; NZ_WYAL01000019.1.
DR AlphaFoldDB; P52688; -.
DR SMR; P52688; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR CDD; cd19925; REC_citrate_TCS; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR048714; DpiA-like_HTH.
DR InterPro; IPR024187; Sig_transdc_resp-reg_cit/mal.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR PANTHER; PTHR45526; TRANSCRIPTIONAL REGULATORY PROTEIN DPIA; 1.
DR PANTHER; PTHR45526:SF1; TRANSCRIPTIONAL REGULATORY PROTEIN DPIA; 1.
DR Pfam; PF20714; HTH_64; 1.
DR Pfam; PF00072; Response_reg; 1.
DR PIRSF; PIRSF006171; RR_citrat_malat; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF52172; CheY-like; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Direct protein sequencing; DNA-binding;
KW Phosphoprotein; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..234
FT /note="Transcriptional regulatory protein CitB"
FT /id="PRO_0000081072"
FT DOMAIN 5..121
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 181..200
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT MOD_RES 56
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT ECO:0000269|PubMed:10447894"
FT MUTAGEN 56
FT /note="D->N: Loss of phosphorylation."
FT /evidence="ECO:0000269|PubMed:10447894"
SQ SEQUENCE 234 AA; 26821 MW; C793725EE30E3DB8 CRC64;
MDSITTLIVE DEPMLAEILV DNIKQFPQFD VIGIADKLES ARKQLRLYQP QLILLDNFLP
DGKGIDLIRH AVSTHYKGRI IFITADNHME TISEALRLGV FDYLIKPVHY QRLQHTLERF
ARYRSSLRSS EQASQLHVDA LFNIQAREQT EPASAPLRGI DESTFQRVLQ LFADPTVVHT
ADSLARILGS SKTTARRYLE QGVKNDFLEA EISYGKVGRP ERIYHGKQTY PEQR
//
