ID YHCN_BACSU Reviewed; 189 AA.
AC P54598;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=Probable spore germination lipoprotein YhcN {ECO:0000305};
DE Flags: Precursor;
GN Name=yhcN; OrderedLocusNames=BSU09150;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969498; DOI=10.1099/13500872-142-11-3021;
RA Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S.;
RT "A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the
RT Bacillus subtilis chromosome.";
RL Microbiology 142:3021-3026(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 66.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP PROTEIN SEQUENCE OF 141-150, FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / PS832, and 168 / PY79;
RX PubMed=9611260; DOI=10.1016/s0378-1119(98)00172-3;
RA Bagyan I., Noback M., Bron S., Paidhungat M., Setlow P.;
RT "Characterization of yhcN, a new forespore-specific gene of Bacillus
RT subtilis.";
RL Gene 212:179-188(1998).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=26731423; DOI=10.1021/acs.jproteome.5b00976;
RA Zheng L., Abhyankar W., Ouwerling N., Dekker H.L., van Veen H.,
RA van der Wel N.N., Roseboom W., de Koning L.J., Brul S., de Koster C.G.;
RT "Bacillus subtilis spore inner membrane proteome.";
RL J. Proteome Res. 15:585-594(2016).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28333204; DOI=10.1093/femsle/fnx047;
RA Johnson C.L., Moir A.;
RT "Proteins YlaJ and YhcN contribute to the efficiency of spore germination
RT in Bacillus subtilis.";
RL FEMS Microbiol. Lett. 364:0-0(2017).
CC -!- FUNCTION: Probably contributes, directly or indirectly, to early events
CC in germination (PubMed:28333204). May play a role in spore outgrowth
CC (PubMed:9611260). {ECO:0000269|PubMed:28333204,
CC ECO:0000269|PubMed:9611260}.
CC -!- SUBCELLULAR LOCATION: Forespore inner membrane
CC {ECO:0000269|PubMed:26731423, ECO:0000269|PubMed:9611260}; Lipid-anchor
CC {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore during sporulation.
CC {ECO:0000269|PubMed:9611260}.
CC -!- INDUCTION: Expression is dependent on sigma G, and to a much lesser
CC extent on sigma F. {ECO:0000269|PubMed:9611260}.
CC -!- DISRUPTION PHENOTYPE: Mutant sporulates normally and has the same
CC resistance to heat and UV radiation than wild-type spores. It exhibits
CC no defect in the initiation of spore germination, but the spore
CC outgrowth is slower than that of wild-type spores (PubMed:9611260).
CC Mutant shows a reduced rate of spore germination in L-alanine
CC (PubMed:28333204). {ECO:0000269|PubMed:28333204,
CC ECO:0000269|PubMed:9611260}.
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DR EMBL; X96983; CAA65698.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12743.2; -; Genomic_DNA.
DR PIR; B69823; B69823.
DR RefSeq; NP_388796.2; NC_000964.3.
DR RefSeq; WP_003245125.1; NZ_JNCM01000035.1.
DR PDB; 7PEG; X-ray; 1.77 A; A/B=24-189.
DR PDBsum; 7PEG; -.
DR AlphaFoldDB; P54598; -.
DR SMR; P54598; -.
DR STRING; 224308.BSU09150; -.
DR PaxDb; 224308-BSU09150; -.
DR EnsemblBacteria; CAB12743; CAB12743; BSU_09150.
DR GeneID; 939739; -.
DR KEGG; bsu:BSU09150; -.
DR PATRIC; fig|224308.179.peg.989; -.
DR eggNOG; ENOG5032V70; Bacteria.
DR InParanoid; P54598; -.
DR OrthoDB; 1707228at2; -.
DR BioCyc; BSUB:BSU09150-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:InterPro.
DR InterPro; IPR014247; Spore_lipoprot_YhcN/YlaJ.
DR InterPro; IPR019076; Spore_lipoprot_YhcN/YlaJ-like.
DR NCBIfam; TIGR02898; spore_YhcN_YlaJ; 1.
DR Pfam; PF09580; Spore_YhcN_YlaJ; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Germination; Lipoprotein;
KW Membrane; Palmitate; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 21..189
FT /note="Probable spore germination lipoprotein YhcN"
FT /id="PRO_0000018062"
FT REGION 24..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 21
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 21
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CONFLICT 66
FT /note="V -> A (in Ref. 1; CAA65698)"
FT /evidence="ECO:0000305"
FT HELIX 85..91
FT /evidence="ECO:0007829|PDB:7PEG"
FT STRAND 97..105
FT /evidence="ECO:0007829|PDB:7PEG"
FT STRAND 108..115
FT /evidence="ECO:0007829|PDB:7PEG"
FT HELIX 125..136
FT /evidence="ECO:0007829|PDB:7PEG"
FT STRAND 144..148
FT /evidence="ECO:0007829|PDB:7PEG"
FT HELIX 151..166
FT /evidence="ECO:0007829|PDB:7PEG"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:7PEG"
FT HELIX 174..184
FT /evidence="ECO:0007829|PDB:7PEG"
SQ SEQUENCE 189 AA; 21016 MW; 8C14FD9DBC64E32D CRC64;
MFGKKQVLAS VLLIPLLMTG CGVADQGEGR RDNNDVRNVN YRNPANDDMR NVNNRDNVDN
NVNDNVNNNR VNDDNNNDRK LEVADEAADK VTDLKEVKHA DIIVAGNQAY VAVVLTNGNK
GAVENNLKKK IAKKVRSTDK NIDNVYVSAN PDFVERMQGY GKRIQNGDPI AGLFDEFTQT
VQRVFPNAE
//
