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Database: UniProt
Entry: P56255
LinkDB: P56255
Original site: P56255 
ID   PCRA_GEOSE              Reviewed;         724 AA.
AC   P56255;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 1.
DT   28-FEB-2018, entry version 90.
DE   RecName: Full=ATP-dependent DNA helicase PcrA;
DE            EC=3.6.4.12;
GN   Name=pcrA;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   CHARACTERIZATION.
RX   PubMed=9592155; DOI=10.1093/nar/26.11.2686;
RA   Bird L.E., Brannigan J.A., Subramanya H.S., Wigley D.B.;
RT   "Characterisation of Bacillus stearothermophilus PcrA helicase:
RT   evidence against an active rolling mechanism.";
RL   Nucleic Acids Res. 26:2686-2693(1998).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RC   STRAIN=ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924;
RX   PubMed=8934527; DOI=10.1038/384379a0;
RA   Subramanya H.S., Bird L.E., Brannigan J.A., Wigley D.B.;
RT   "Crystal structure of a DExx box DNA helicase.";
RL   Nature 384:379-383(1996).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
RX   PubMed=10199404; DOI=10.1016/S0092-8674(00)80716-3;
RA   Velankar S.S., Soultanas P., Dillingham M.S., Subramanya H.S.,
RA   Wigley D.B.;
RT   "Crystal structures of complexes of PcrA DNA helicase with a DNA
RT   substrate indicate an inchworm mechanism.";
RL   Cell 97:75-84(1999).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) IN COMPLEX WITH ATP.
RX   PubMed=10388562; DOI=10.1006/jmbi.1999.2873;
RA   Soultanas P., Dillingham M.S., Velankar S.S., Wigley D.B.;
RT   "DNA binding mediates conformational changes and metal ion
RT   coordination in the active site of PcrA helicase.";
RL   J. Mol. Biol. 290:137-148(1999).
RN   [5]
RP   MUTAGENESIS.
RX   PubMed=10454638; DOI=10.1093/nar/27.16.3310;
RA   Dillingham M.S., Soultanas P., Wigley D.B.;
RT   "Site-directed mutagenesis of motif III in PcrA helicase reveals a
RT   role in coupling ATP hydrolysis to strand separation.";
RL   Nucleic Acids Res. 27:3310-3317(1999).
CC   -!- FUNCTION: DNA helicase. Has a broad nucleotide specificity, even
CC       being able to hydrolyze ethenonucleotides, and is able to couple
CC       the hydrolysis to unwinding of DNA substrates. It is a 3'-5'
CC       helicase but at high protein concentrations it can also displace a
CC       substrate with a 5' tail. Preferred substrate being one with both
CC       single and double-stranded regions of DNA.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:10388562}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
DR   PDB; 1PJR; X-ray; 2.50 A; A=1-724.
DR   PDB; 1QHG; X-ray; 2.50 A; A=1-724.
DR   PDB; 1QHH; X-ray; 2.50 A; A=1-167, B=168-440, C=441-555, D=556-724.
DR   PDB; 2PJR; X-ray; 2.90 A; A/F=1-548, B/G=556-650.
DR   PDB; 3PJR; X-ray; 3.30 A; A=1-724.
DR   PDB; 5DMA; X-ray; 1.53 A; A=673-724.
DR   PDBsum; 1PJR; -.
DR   PDBsum; 1QHG; -.
DR   PDBsum; 1QHH; -.
DR   PDBsum; 2PJR; -.
DR   PDBsum; 3PJR; -.
DR   PDBsum; 5DMA; -.
DR   ProteinModelPortal; P56255; -.
DR   SMR; P56255; -.
DR   BRENDA; 3.6.4.12; 623.
DR   EvolutionaryTrace; P56255; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   Gene3D; 1.10.10.160; -; 1.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN         1    724       ATP-dependent DNA helicase PcrA.
FT                                /FTId=PRO_0000102050.
FT   DOMAIN       10    289       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   DOMAIN      290    569       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00617}.
FT   NP_BIND      11     16       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560,
FT                                ECO:0000269|PubMed:10388562}.
FT   NP_BIND      34     39       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560,
FT                                ECO:0000269|PubMed:10388562}.
FT   BINDING     287    287       ATP. {ECO:0000269|PubMed:10388562}.
FT   HELIX         3      9       {ECO:0000244|PDB:1PJR}.
FT   HELIX        14     21       {ECO:0000244|PDB:1PJR}.
FT   STRAND       27     31       {ECO:0000244|PDB:1PJR}.
FT   HELIX        37     50       {ECO:0000244|PDB:1PJR}.
FT   HELIX        56     58       {ECO:0000244|PDB:1PJR}.
FT   STRAND       59     65       {ECO:0000244|PDB:1PJR}.
FT   HELIX        66     80       {ECO:0000244|PDB:1PJR}.
FT   HELIX        81     84       {ECO:0000244|PDB:1PJR}.
FT   STRAND       87     91       {ECO:0000244|PDB:1PJR}.
FT   HELIX        92    103       {ECO:0000244|PDB:1PJR}.
FT   HELIX       104    107       {ECO:0000244|PDB:1PJR}.
FT   STRAND      114    116       {ECO:0000244|PDB:2PJR}.
FT   HELIX       118    130       {ECO:0000244|PDB:1PJR}.
FT   TURN        135    138       {ECO:0000244|PDB:1PJR}.
FT   HELIX       141    153       {ECO:0000244|PDB:1PJR}.
FT   HELIX       158    161       {ECO:0000244|PDB:1PJR}.
FT   HELIX       169    186       {ECO:0000244|PDB:1PJR}.
FT   STRAND      189    191       {ECO:0000244|PDB:2PJR}.
FT   HELIX       194    205       {ECO:0000244|PDB:1PJR}.
FT   HELIX       207    216       {ECO:0000244|PDB:1PJR}.
FT   STRAND      218    224       {ECO:0000244|PDB:1PJR}.
FT   HELIX       225    227       {ECO:0000244|PDB:1PJR}.
FT   HELIX       230    240       {ECO:0000244|PDB:1PJR}.
FT   TURN        241    243       {ECO:0000244|PDB:1PJR}.
FT   STRAND      246    250       {ECO:0000244|PDB:1PJR}.
FT   HELIX       252    254       {ECO:0000244|PDB:1PJR}.
FT   HELIX       258    260       {ECO:0000244|PDB:1PJR}.
FT   HELIX       265    268       {ECO:0000244|PDB:1PJR}.
FT   HELIX       270    273       {ECO:0000244|PDB:1PJR}.
FT   STRAND      278    282       {ECO:0000244|PDB:1PJR}.
FT   STRAND      286    288       {ECO:0000244|PDB:1PJR}.
FT   HELIX       290    300       {ECO:0000244|PDB:1PJR}.
FT   STRAND      304    306       {ECO:0000244|PDB:2PJR}.
FT   STRAND      312    315       {ECO:0000244|PDB:2PJR}.
FT   STRAND      321    328       {ECO:0000244|PDB:1PJR}.
FT   HELIX       329    344       {ECO:0000244|PDB:1PJR}.
FT   TURN        345    347       {ECO:0000244|PDB:1PJR}.
FT   HELIX       351    353       {ECO:0000244|PDB:1PJR}.
FT   STRAND      354    360       {ECO:0000244|PDB:1PJR}.
FT   HELIX       361    363       {ECO:0000244|PDB:1PJR}.
FT   HELIX       364    373       {ECO:0000244|PDB:1PJR}.
FT   STRAND      378    381       {ECO:0000244|PDB:1PJR}.
FT   HELIX       386    388       {ECO:0000244|PDB:1PJR}.
FT   HELIX       390    403       {ECO:0000244|PDB:1PJR}.
FT   HELIX       408    414       {ECO:0000244|PDB:1PJR}.
FT   STRAND      415    418       {ECO:0000244|PDB:2PJR}.
FT   TURN        421    423       {ECO:0000244|PDB:1PJR}.
FT   HELIX       424    435       {ECO:0000244|PDB:2PJR}.
FT   TURN        436    438       {ECO:0000244|PDB:2PJR}.
FT   TURN        442    446       {ECO:0000244|PDB:1PJR}.
FT   TURN        449    451       {ECO:0000244|PDB:1PJR}.
FT   TURN        454    461       {ECO:0000244|PDB:1PJR}.
FT   HELIX       462    474       {ECO:0000244|PDB:1PJR}.
FT   TURN        475    477       {ECO:0000244|PDB:1PJR}.
FT   HELIX       480    490       {ECO:0000244|PDB:1PJR}.
FT   HELIX       493    500       {ECO:0000244|PDB:1PJR}.
FT   HELIX       503    525       {ECO:0000244|PDB:1PJR}.
FT   HELIX       531    537       {ECO:0000244|PDB:1PJR}.
FT   TURN        538    540       {ECO:0000244|PDB:1PJR}.
FT   TURN        544    546       {ECO:0000244|PDB:2PJR}.
FT   STRAND      559    563       {ECO:0000244|PDB:1PJR}.
FT   HELIX       564    567       {ECO:0000244|PDB:1PJR}.
FT   STRAND      572    577       {ECO:0000244|PDB:1PJR}.
FT   TURN        584    586       {ECO:0000244|PDB:1PJR}.
FT   HELIX       588    591       {ECO:0000244|PDB:1PJR}.
FT   HELIX       594    608       {ECO:0000244|PDB:1PJR}.
FT   STRAND      611    625       {ECO:0000244|PDB:1PJR}.
FT   STRAND      628    630       {ECO:0000244|PDB:1PJR}.
FT   HELIX       636    640       {ECO:0000244|PDB:1PJR}.
FT   HELIX       643    645       {ECO:0000244|PDB:1PJR}.
FT   STRAND      646    648       {ECO:0000244|PDB:1PJR}.
FT   STRAND      678    681       {ECO:0000244|PDB:5DMA}.
FT   TURN        682    684       {ECO:0000244|PDB:5DMA}.
FT   STRAND      685    694       {ECO:0000244|PDB:5DMA}.
FT   HELIX       696    698       {ECO:0000244|PDB:5DMA}.
FT   STRAND      700    705       {ECO:0000244|PDB:5DMA}.
FT   TURN        706    709       {ECO:0000244|PDB:5DMA}.
FT   STRAND      710    716       {ECO:0000244|PDB:5DMA}.
FT   STRAND      720    723       {ECO:0000244|PDB:5DMA}.
SQ   SEQUENCE   724 AA;  82488 MW;  6D20CA468BFB4291 CRC64;
     MNFLSEQLLA HLNKEQQEAV RTTEGPLLIM AGAGSGKTRV LTHRIAYLMA EKHVAPWNIL
     AITFTNKAAR EMRERVQSLL GGAAEDVWIS TFHSMCVRIL RRDIDRIGIN RNFSILDPTD
     QLSVMKTILK EKNIDPKKFE PRTILGTISA AKNELLPPEQ FAKRASTYYE KVVSDVYQEY
     QQRLLRNHSL DFDDLIMTTI QLFDRVPDVL HYYQYKFQYI HIDEYQDTNR AQYTLVKKLA
     ERFQNICAVG DADQSIYRWR GADIQNILSF ERDYPNAKVI LLEQNYRSTK RILQAANEVI
     EHNVNRKPKR IWTENPEGKP ILYYEAMNEA DEAQFVAGRI REAVERGERR YRDFAVLYRT
     NAQSRVMEEM LLKANIPYQI VGGLKFYDRK EIKDILAYLR VIANPDDDLS LLRIINVPKR
     GIGASTIDKL VRYAADHELS LFEALGELEM IGLGAKAAGA LAAFRSQLEQ WTQLQEYVSV
     TELVEEVLDK SGYREMLKAE RTIEAQSRLE NLDEFLSVTK HFENVSDDKS LIAFLTDLAL
     ISDLDELDGT EQAAEGDAVM LMTLHAAKGL EFPVVFLIGM EEGIFPHNRS LEDDDEMEEE
     RRLAYVGITR AEEELVLTSA QMRTLFGNIQ MDPPSRFLNE IPAHLLETAS RRQAGASRPA
     VSRPQASGAV GSWKVGDRAN HRKWGIGTVV SVRGGGDDQE LDIAFPSPIG IKRLLAKFAP
     IEKV
//
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