ID RSUA_SALTY Reviewed; 231 AA.
AC P65840; Q8XGP8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 27-MAR-2024, entry version 98.
DE RecName: Full=Ribosomal small subunit pseudouridine synthase A;
DE EC=5.4.99.19;
DE AltName: Full=16S pseudouridylate 516 synthase;
DE AltName: Full=16S rRNA pseudouridine(516) synthase;
DE AltName: Full=rRNA pseudouridylate synthase A;
DE AltName: Full=rRNA-uridine isomerase A;
GN Name=rsuA; OrderedLocusNames=STM2222;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-516 in
CC 16S ribosomal RNA. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(516) in 16S rRNA = pseudouridine(516) in 16S rRNA;
CC Xref=Rhea:RHEA:38867, Rhea:RHEA-COMP:10089, Rhea:RHEA-COMP:10090,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.19;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000305}.
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DR EMBL; AE006468; AAL21125.1; -; Genomic_DNA.
DR RefSeq; NP_461166.1; NC_003197.2.
DR RefSeq; WP_001234836.1; NC_003197.2.
DR AlphaFoldDB; P65840; -.
DR SMR; P65840; -.
DR STRING; 99287.STM2222; -.
DR PaxDb; 99287-STM2222; -.
DR GeneID; 1253744; -.
DR KEGG; stm:STM2222; -.
DR PATRIC; fig|99287.12.peg.2354; -.
DR HOGENOM; CLU_024979_1_2_6; -.
DR OMA; QGKYHQV; -.
DR PhylomeDB; P65840; -.
DR BioCyc; SENT99287:STM2222-MONOMER; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02553; PseudoU_synth_RsuA; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR PANTHER; PTHR47683:SF4; PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT CHAIN 1..231
FT /note="Ribosomal small subunit pseudouridine synthase A"
FT /id="PRO_0000099973"
FT DOMAIN 1..68
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 102
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 231 AA; 25760 MW; 29EF9A7C5BD1DEF5 CRC64;
MRLDKFIAQQ LGVSRAIAGR EIRGNRVTVD GDIIKNAAFK LLPEHAVAYD GNPLAQQHGP
RYFMLNKPQG YVCSTDDPDH PTVLYFLDEP VAYKLHAAGR LDIDTTGLVL MTDDGQWSHR
ITSPRHHCEK TYLVTLESPV ADDTAAQFAK GVQLHNEKDL TKPATLEVIT PVQVRLTISE
GRYHQVKRMF AAVGNRVVEL HRERIGAITL DENLAPGEYR PLTEEEIASV G
//
