GenomeNet

Database: UniProt
Entry: P81594
LinkDB: P81594
Original site: P81594 
ID   CPRA_DESHD              Reviewed;         445 AA.
AC   P81594; B8FW15; Q8RPI9;
DT   13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 2.
DT   23-MAY-2018, entry version 83.
DE   RecName: Full=3-chloro-4-hydroxyphenylacetate reductive dehalogenase;
DE            EC=3.8.1.-;
DE   AltName: Full=C1-OHPA dehalogenase;
DE   AltName: Full=O-chlorophenol reductive dehalogenase;
DE   Flags: Precursor;
GN   Name=cprA; OrderedLocusNames=Dhaf_0737;
OS   Desulfitobacterium hafniense (strain DCB-2 / DSM 10664).
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Peptococcaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=272564;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Davis J.K., Tiedje J.M.;
RT   "Sequence and transcriptional analysis of reductive dehalogenase genes
RT   of Desulfitobacterium.";
RL   Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DCB-2 / DSM 10664;
RX   PubMed=22316246; DOI=10.1186/1471-2180-12-21;
RA   Kim S.H., Harzman C., Davis J.K., Hutcheson R., Broderick J.B.,
RA   Marsh T.L., Tiedje J.M.;
RT   "Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-
RT   positive anaerobe capable of dehalogenation and metal reduction.";
RL   BMC Microbiol. 12:21-21(2012).
RN   [3]
RP   PROTEIN SEQUENCE OF 43-66, AND CHARACTERIZATION.
RX   PubMed=9781670; DOI=10.1016/S0014-5793(98)01114-4;
RA   Christiansen N., Ahring B.K., Wohlfarth G., Diekert G.;
RT   "Purification and characterization of the 3-chloro-4-hydroxy-
RT   phenylacetate reductive dehalogenase of Desulfitobacterium
RT   hafniense.";
RL   FEBS Lett. 436:159-162(1998).
CC   -!- FUNCTION: Catalyzes the reductive dechlorination of C1-OHPA to 4-
CC       hydroxyphenylacetate with reduced methyl viologen as the electron
CC       donor.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster.;
CC   -!- COFACTOR:
CC       Name=corrinoid; Xref=ChEBI:CHEBI:33913;
CC       Note=Corrinoid.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has been experimentally proven.
DR   EMBL; AF403182; AAL87763.1; -; Genomic_DNA.
DR   EMBL; CP001336; ACL18801.1; -; Genomic_DNA.
DR   RefSeq; WP_015942994.1; NC_011830.1.
DR   ProteinModelPortal; P81594; -.
DR   PRIDE; P81594; -.
DR   EnsemblBacteria; ACL18801; ACL18801; Dhaf_0737.
DR   KEGG; dhd:Dhaf_0737; -.
DR   HOGENOM; HOG000087131; -.
DR   KO; K21566; -.
DR   BioCyc; DHAF272564:G1GUH-758-MONOMER; -.
DR   Proteomes; UP000007726; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR012832; RDH.
DR   InterPro; IPR028894; RDH_dom.
DR   InterPro; IPR006311; TAT_signal.
DR   InterPro; IPR019546; TAT_signal_bac_arc.
DR   Pfam; PF13486; Dehalogenase; 1.
DR   Pfam; PF12838; Fer4_7; 1.
DR   TIGRFAMs; TIGR02486; RDH; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 2.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Cell membrane; Complete proteome; Direct protein sequencing;
KW   Hydrolase; Iron; Iron-sulfur; Membrane; Metal-binding; Repeat; Signal.
FT   SIGNAL        1     42       Tat-type signal. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00648,
FT                                ECO:0000269|PubMed:9781670}.
FT   CHAIN        43    445       3-chloro-4-hydroxyphenylacetate reductive
FT                                dehalogenase.
FT                                /FTId=PRO_0000020991.
FT   DOMAIN      320    350       4Fe-4S ferredoxin-type 1.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   DOMAIN      368    397       4Fe-4S ferredoxin-type 2.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00711}.
FT   METAL       330    330       Iron-sulfur (4Fe-4S). {ECO:0000255}.
FT   METAL       333    333       Iron-sulfur (4Fe-4S). {ECO:0000255}.
FT   METAL       336    336       Iron-sulfur (4Fe-4S). {ECO:0000255}.
FT   METAL       340    340       Iron-sulfur (4Fe-4S). {ECO:0000255}.
SQ   SEQUENCE   445 AA;  49363 MW;  928BAA0BD6767C12 CRC64;
     MENNQKRQQS GMSRRSFLKV GAAATTMGVI GAIKAPAKVA NAAETLNYVP GSGKIRSKLR
     PVHDFAGAKV RFVENNDQWL GTTKILTKVK MTSEADAGFM QAVRGLYGPE PQKGFFQFIA
     KDPFGGSISW ARNLIAPEDV VDGAPEATKT PIPDPEQMSQ HIRDCCYFLR ADEVGIGKMP
     EYGYYTHHVA DTVGLMTKPV EECVTPVTKI YPNVIVVMID QGIETMWAST GYDGISGAMS
     MKSYFTSGCI AVILAKYIRT LGYNARAHHA KNYEAIMPVC IMAAGLGELS RTGDSAIHPR
     LGFRHKVAAV TTDLPLAPDQ PLDFGLLDFC RVCKKCADNC PNEAISFDED PIEYNGYLRW
     NSDFRKCTEF RTTNEEGSSC GTCMKVCPWN SKEDSWFHKA GVWVGSKGET ASTFLKSIDD
     IFGYGTETIE KYKWWLEWPE KYVMK
//
DBGET integrated database retrieval system