ID   PALST_EUGGR             Reviewed;        1019 AA.
AC   P84739; Q2P9M8;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 2.
DT   22-FEB-2023, entry version 64.
DE   RecName: Full=Photoactivated adenylate cyclase subunit alpha-like protein ST-;
GN   Name=pacA {ECO:0000312|EMBL:CAJ57396.1};
OS   Euglena gracilis.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3039;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ST- {ECO:0000269|PubMed:16121285};
RX   PubMed=16121285; DOI=10.1039/b502002f;
RA   Ntefidou M., Haeder D.-P.;
RT   "Photoactivated adenylyl cyclase (PAC) genes in the flagellate Euglena
RT   gracilis mutant strains.";
RL   Photochem. Photobiol. Sci. 4:732-739(2005).
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC       {ECO:0000250|UniProtKB:Q8S9F2}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum
CC       {ECO:0000269|PubMed:16121285}.
CC   -!- MISCELLANEOUS: The ST- strain is deficient in phototaxis. It is not
CC       known if this is due to defective adenylate cyclase activity or
CC       defective BLUF domains in this protein. In wild-type E.gracilis,
CC       photoactivated adenylate cyclase is found in the paraxonemal bodies
CC       (PABs). PABs are absent from this strain.
CC   -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC       family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
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DR   EMBL; AM181337; CAJ57396.1; -; mRNA.
DR   AlphaFoldDB; P84739; -.
DR   SMR; P84739; -.
DR   GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR   GO; GO:0009882; F:blue light photoreceptor activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0009190; P:cyclic nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR   CDD; cd07302; CHD; 2.
DR   Gene3D; 3.30.70.100; -; 2.
DR   Gene3D; 3.30.70.1230; Nucleotide cyclase; 2.
DR   InterPro; IPR001054; A/G_cyclase.
DR   InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR   InterPro; IPR007024; BLUF_domain.
DR   InterPro; IPR029787; Nucleotide_cyclase.
DR   Pfam; PF04940; BLUF; 2.
DR   Pfam; PF00211; Guanylate_cyc; 2.
DR   SMART; SM01034; BLUF; 2.
DR   SUPFAM; SSF54975; Acylphosphatase/BLUF domain-like; 2.
DR   SUPFAM; SSF55073; Nucleotide cyclase; 2.
DR   PROSITE; PS50925; BLUF; 2.
DR   PROSITE; PS50125; GUANYLATE_CYCLASE_2; 2.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Flagellum; Repeat.
FT   CHAIN           1..1019
FT                   /note="Photoactivated adenylate cyclase subunit alpha-like
FT                   protein ST-"
FT                   /id="PRO_0000195721"
FT   DOMAIN          55..148
FT                   /note="BLUF 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          204..332
FT                   /note="Guanylate cyclase 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   DOMAIN          467..559
FT                   /note="BLUF 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00030"
FT   DOMAIN          615..744
FT                   /note="Guanylate cyclase 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT   REGION          801..846
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          887..923
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          963..993
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        801..824
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1019 AA;  112470 MW;  49149819DE0A9435 CRC64;
     MYNRVYKERQ EIRTIQGLEA SGQFQTASNI TDIQIYSINV TPTMSKGGET GETQLRRLMY
     LSASTEPEKC NAEYLADMAH VATLRNKAIG VIRFLLYSSP FFFQVIEGTD EDLDFLFAKI
     SADPRHERCI VLANGPCTGR MYGEWHMKDS HIDNITNNPA IKTILFQIAR SFTSMWSYLP
     KNAANMLLLG KNPNKQGPEP RSVVLTFIYL VEFSSILAHP GLTEQCADIL AAFVEASVRN
     CEGTWGLVAK FITGTCVAYW PINRAEDALV GLQQLSEDLA VLRSYQPPGS ALSRIYSRRG
     VHYGRALLCN AGFRKADFTL LGDCINTASR ITSLSVKLKV PLLLSFEVRC LLGDEMREEL
     ERSALHKVKG RDKPVQVYQF NAPELDSALV RAKIEQFNPG RYRALCPVKP YESLHPAQRP
     PIFDDTPREN QPKLSQVQRR DSLVDRLSLI AKLAFPSSMM AGGEGQLITL TYISQAAHPM
     SRFDLASIQR IAFARNESSN ITGSLLYVSG LFVQTLDDPK GAVESLYLKI RQDKRHKDVV
     AVFMAPIDER VYGTPLDMTS ATEEMLATFP PLQDVLSQLA KSFISLETYV PSTVVRYLTA
     GNNPRNLQPV SVEVVMLATD ICSFTPLSEK CSLTEVWTIC NTFIDACTSA ICNEGGEVIK
     LIGDCVTAYF APTGADNAVH ACQEIVSFSA QLRDAFHDVL DCRSVVACGV GLDFGQVIMA
     QCGSLRLTEF VVAGEVSARV MAVEALTREA GRAIVITEPV SDRLSAKLRD TGIVPCQEGL
     HAVLLWYPGP RMGIGCANHQ EDHLRVPRRP GPGRHEEGGR RHQRPGPGRP RRGHPFLPQG
     STTRPHQFSV QLHPLPKLGT GSSDGGIGVS LHVYSAGRYR QQLHRRQIAA GGQRRSVGPG
     QDVARATRAH ACDAGHQAPD QPANAQYERQ FRGGQQRWRT CGIVHSNAFV TGVGLVQQPW
     LDEGHRPEAP HQSLHPGPGD PSEQHPHRPN RATEPGLLVA RQPAVCLHRP EGFAQICTF
//