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Database: UniProt
Entry: P94026
LinkDB: P94026
Original site: P94026 
ID   RBCMT_TOBAC             Reviewed;         491 AA.
AC   P94026;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   05-DEC-2018, entry version 87.
DE   RecName: Full=Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic;
DE            EC=2.1.1.127;
DE   AltName: Full=[Ribulose-bisphosphate carboxylase]-lysine N-methyltransferase;
DE            Short=RuBisCO LSMT;
DE            Short=RuBisCO methyltransferase;
DE            Short=rbcMT;
DE   Flags: Precursor;
GN   Name=RBCMT;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae;
OC   Pentapetalae; asterids; lamiids; Solanales; Solanaceae;
OC   Nicotianoideae; Nicotianeae; Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. KY14;
RX   PubMed=8980518; DOI=10.1007/BF00020207;
RA   Ying Z., Janney N., Houtz R.L.;
RT   "Organization and characterization of the ribulose-1,5-bisphosphate
RT   carboxylase/oxygenase large subunit epsilon N-methyltransferase gene
RT   in tobacco.";
RL   Plant Mol. Biol. 32:663-671(1996).
CC   -!- FUNCTION: Methylates 'Lys-14' of the large subunit of RuBisCO.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[ribulose-1,5-bisphosphate carboxylase]-L-lysine + 3 S-
CC         adenosyl-L-methionine = [ribulose-1,5-bisphosphate carboxylase]-
CC         N(6),N(6),N(6)-trimethyl-L-lysine + 3 H(+) + 3 S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:50996, Rhea:RHEA-COMP:12858,
CC         Rhea:RHEA-COMP:12859, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:61961;
CC         EC=2.1.1.127;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the class V-like SAM-binding
CC       methyltransferase superfamily. Plant protein-lysine LSMT
CC       methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU00916}.
DR   EMBL; U35620; AAC49566.1; -; mRNA.
DR   EMBL; U35619; AAC49565.1; -; Genomic_DNA.
DR   PIR; T03759; T03759.
DR   RefSeq; NP_001312185.1; NM_001325256.1.
DR   UniGene; Nta.3628; -.
DR   ProteinModelPortal; P94026; -.
DR   SMR; P94026; -.
DR   GeneID; 107778201; -.
DR   KEGG; ag:AAC49566; -.
DR   KEGG; nta:107778201; -.
DR   KO; K00592; -.
DR   Proteomes; UP000084051; Genome assembly.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0030785; F:[ribulose-bisphosphate carboxylase]-lysine N-methyltransferase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.90.1420.10; -; 1.
DR   InterPro; IPR011192; Rubisco_LSMT_MeTrfase_plant.
DR   InterPro; IPR015353; Rubisco_LSMT_subst-bd.
DR   InterPro; IPR036464; Rubisco_LSMT_subst-bd_sf.
DR   InterPro; IPR001214; SET_dom.
DR   Pfam; PF09273; Rubis-subs-bind; 1.
DR   Pfam; PF00856; SET; 1.
DR   PIRSF; PIRSF009328; RMT_SET; 1.
DR   SMART; SM00317; SET; 1.
DR   SUPFAM; SSF81822; SSF81822; 1.
DR   PROSITE; PS51583; SAM_MT127; 1.
DR   PROSITE; PS50280; SET; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Complete proteome; Methyltransferase; Plastid;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase;
KW   Transit peptide.
FT   TRANSIT       1      ?       Chloroplast.
FT   CHAIN         ?    491       Ribulose-1,5 bisphosphate
FT                                carboxylase/oxygenase large subunit N-
FT                                methyltransferase, chloroplastic.
FT                                /FTId=PRO_0000022200.
FT   DOMAIN       67    291       SET. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00190}.
SQ   SEQUENCE   491 AA;  56000 MW;  4EA7DE9E9497D286 CRC64;
     MASVFSVHPL PSSSFLCPLK TTKSRTKHHQ TFYTYQKTIL INSLQLTELD PKIPQPVQTF
     WQWLCKEGVV TTKTPVKPGI VPEGLGLVAK RDIAKGETVL QVPKRFWINP DAVAESEIGN
     VCSGLKPWIS VALFLLREKW RDDSKWKYYM DVLPKSTDST IYWSEEELSE IQGTQLLSTT
     MSVKDYVQNE FQKVEEEVIL RNKQLFPFPI TLDDFFWAFG ILRSRAFSRL RNQNLILVPF
     ADLTNHNARV TTEDHAHEVR GPAGLFSWDL LFSLRSPLKL KAGDQLFIQY DLNKSNADMA
     LDYGFIEPSS ARDAFTLTLE ISESDEFYGD KLDIAETNGI GETAYFDIKI GQSLPPTMIP
     YLRLVALGGT DAFLLESIFR NSVWGHLGLP VSRANEELIC KVVRDACKSA LSGYHTTIEE
     DEKLMEEGNL STRLQIAVGI RLGEKRVLKQ IDDIFREREL ELDELEYYGE RRLKDLGLVG
     EQGDIIFWEP K
//
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