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Database: UniProt
Entry: P9WFC6
LinkDB: P9WFC6
Original site: P9WFC6 
ID   UVRB_MYCTO              Reviewed;         719 AA.
AC   P9WFC6; L0T7H2; O06150; P67422;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   04-FEB-2015, sequence version 2.
DT   16-OCT-2019, entry version 33.
DE   RecName: Full=UvrABC system protein B {ECO:0000255|HAMAP-Rule:MF_00204};
DE            Short=Protein UvrB {ECO:0000255|HAMAP-Rule:MF_00204};
DE   AltName: Full=Excinuclease ABC subunit B {ECO:0000255|HAMAP-Rule:MF_00204};
GN   Name=uvrB {ECO:0000255|HAMAP-Rule:MF_00204}; OrderedLocusNames=MT1669;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H.,
RA   Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D.,
RA   Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M.,
RA   Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C.,
RA   Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC       processing of DNA lesions. A damage recognition complex composed
CC       of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon
CC       binding of the UvrA(2)B(2) complex to a putative damaged site, the
CC       DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP
CC       binding by UvrB and probably causes local melting of the DNA
CC       helix, facilitating insertion of UvrB beta-hairpin between the DNA
CC       strands. Then UvrB probes one DNA strand for the presence of a
CC       lesion. If a lesion is found the UvrA subunits dissociate and the
CC       UvrB-DNA preincision complex is formed. This complex is
CC       subsequently bound by UvrC and the second UvrB is released. If no
CC       lesion is found, the DNA wraps around the other UvrB subunit that
CC       will check the other stand for damage. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC       lesions. Interacts with UvrC in an incision complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- DOMAIN: The beta-hairpin motif is involved in DNA binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SIMILARITY: Belongs to the UvrB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK45939.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
DR   EMBL; AE000516; AAK45939.1; ALT_INIT; Genomic_DNA.
DR   PIR; G70559; G70559.
DR   SMR; P9WFC6; -.
DR   EnsemblBacteria; AAK45939; AAK45939; MT1669.
DR   KEGG; mtc:MT1669; -.
DR   KO; K03702; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00204; UvrB; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   InterPro; IPR036876; UVR_dom_sf.
DR   InterPro; IPR004807; UvrB.
DR   InterPro; IPR041471; UvrB_inter.
DR   InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR   PANTHER; PTHR24029; PTHR24029; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF02151; UVR; 1.
DR   Pfam; PF12344; UvrB; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46600; SSF46600; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00631; uvrb; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA damage; DNA excision;
KW   DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
FT   CHAIN         1    719       UvrABC system protein B.
FT                                /FTId=PRO_0000428562.
FT   DOMAIN       49    435       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      453    606       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00204}.
FT   DOMAIN      674    709       UVR. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   NP_BIND      62     69       ATP. {ECO:0000255|HAMAP-Rule:MF_00204}.
FT   MOTIF       115    138       Beta-hairpin.
SQ   SEQUENCE   719 AA;  80407 MW;  6841F648049294A8 CRC64;
     MAFATEHPVV AHSEYRAVEE IVRAGGHFEV VSPHAPAGDQ PAAIDELERR INAGERDVVL
     LGATGTGKSA TTAWLIERLQ RPTLVMAPNK TLAAQLANEL REMLPHNAVE YFVSYYDYYQ
     PEAYIAQTDT YIEKDSSIND DVERLRHSAT SALLSRRDVV VVASVSCIYG LGTPQSYLDR
     SVELKVGEEV PRDGLLRLLV DVQYTRNDMS FTRGSFRVRG DTVEIIPSYE ELAVRIEFFG
     DEIEALYYLH PLTGEVIRQV DSLRIFPATH YVAGPERMAH AVSAIEEELA ERLAELESQG
     KLLEAQRLRM RTNYDIEMMR QVGFCSGIEN YSRHIDGRGP GTPPATLLDY FPEDFLLVID
     ESHVTVPQIG GMYEGDISRK RNLVEYGFRL PSACDNRPLT WEEFADRIGQ TVYLSATPGP
     YELSQTGGEF VEQVIRPTGL VDPKVVVKPT KGQIDDLIGE IRTRADADQR VLVTTLTKKM
     AEDLTDYLLE MGIRVRYLHS EVDTLRRVEL LRQLRLGDYD VLVGINLLRE GLDLPEVSLV
     AILDADKEGF LRSSRSLIQT IGRAARNVSG EVHMYADKIT DSMREAIDET ERRRAKQIAY
     NEANGIDPQP LRKKIADILD QVYREADDTA VVEVGGSGRN ASRGRRAQGE PGRAVSAGVF
     EGRDTSAMPR AELADLIKDL TAQMMAAARD LQFELAARFR DEIADLKREL RGMDAAGLK
//
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