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Database: UniProt
Entry: P9WFC7
LinkDB: P9WFC7
Original site: P9WFC7 
ID   UVRB_MYCTU              Reviewed;         719 AA.
AC   P9WFC7; L0T7H2; O06150; P67422;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   04-FEB-2015, sequence version 2.
DT   11-DEC-2019, entry version 36.
DE   RecName: Full=UvrABC system protein B {ECO:0000255|HAMAP-Rule:MF_00204};
DE            Short=Protein UvrB {ECO:0000255|HAMAP-Rule:MF_00204};
DE   AltName: Full=Excinuclease ABC subunit B {ECO:0000255|HAMAP-Rule:MF_00204};
GN   Name=uvrB {ECO:0000255|HAMAP-Rule:MF_00204}; OrderedLocusNames=Rv1633;
GN   ORFNames=MTCY01B2.25;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC       processing of DNA lesions. A damage recognition complex composed of 2
CC       UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of
CC       the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps
CC       around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB
CC       and probably causes local melting of the DNA helix, facilitating
CC       insertion of UvrB beta-hairpin between the DNA strands. Then UvrB
CC       probes one DNA strand for the presence of a lesion. If a lesion is
CC       found the UvrA subunits dissociate and the UvrB-DNA preincision complex
CC       is formed. This complex is subsequently bound by UvrC and the second
CC       UvrB is released. If no lesion is found, the DNA wraps around the other
CC       UvrB subunit that will check the other stand for damage.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBUNIT: Forms a heterotetramer with UvrA during the search for
CC       lesions. Interacts with UvrC in an incision complex.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- DOMAIN: The beta-hairpin motif is involved in DNA binding.
CC       {ECO:0000255|HAMAP-Rule:MF_00204}.
CC   -!- SIMILARITY: Belongs to the UvrB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00204}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP44397.1; Type=Erroneous initiation; Note=Truncated N-terminus.;
DR   EMBL; AL123456; CCP44397.1; ALT_INIT; Genomic_DNA.
DR   PIR; G70559; G70559.
DR   RefSeq; NP_216149.3; NC_000962.3.
DR   SMR; P9WFC7; -.
DR   STRING; 83332.Rv1633; -.
DR   PaxDb; P9WFC7; -.
DR   PRIDE; P9WFC7; -.
DR   EnsemblBacteria; CCP44397; CCP44397; Rv1633.
DR   GeneID; 885249; -.
DR   KEGG; mtu:Rv1633; -.
DR   PATRIC; fig|83332.12.peg.1818; -.
DR   TubercuList; Rv1633; -.
DR   eggNOG; ENOG4105CCW; Bacteria.
DR   eggNOG; COG0556; LUCA.
DR   KO; K03702; -.
DR   BioCyc; MTBH37RV:G185E-5822-MONOMER; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATPase activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0052556; P:positive regulation by symbiont of host immune response; IDA:MTBBASE.
DR   GO; GO:0051409; P:response to nitrosative stress; IMP:MTBBASE.
DR   GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00204; UvrB; 1.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001943; UVR_dom.
DR   InterPro; IPR036876; UVR_dom_sf.
DR   InterPro; IPR004807; UvrB.
DR   InterPro; IPR041471; UvrB_inter.
DR   InterPro; IPR024759; UvrB_YAD/RRR_dom.
DR   PANTHER; PTHR24029; PTHR24029; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF02151; UVR; 1.
DR   Pfam; PF12344; UvrB; 1.
DR   Pfam; PF17757; UvrB_inter; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF46600; SSF46600; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00631; uvrb; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50151; UVR; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair;
KW   Excision nuclease; Nucleotide-binding; Reference proteome; SOS response.
FT   CHAIN           1..719
FT                   /note="UvrABC system protein B"
FT                   /id="PRO_0000138412"
FT   DOMAIN          49..435
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00204"
FT   DOMAIN          453..606
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00204"
FT   DOMAIN          674..709
FT                   /note="UVR"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00204"
FT   NP_BIND         62..69
FT                   /note="ATP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00204"
FT   MOTIF           115..138
FT                   /note="Beta-hairpin"
SQ   SEQUENCE   719 AA;  80407 MW;  6841F648049294A8 CRC64;
     MAFATEHPVV AHSEYRAVEE IVRAGGHFEV VSPHAPAGDQ PAAIDELERR INAGERDVVL
     LGATGTGKSA TTAWLIERLQ RPTLVMAPNK TLAAQLANEL REMLPHNAVE YFVSYYDYYQ
     PEAYIAQTDT YIEKDSSIND DVERLRHSAT SALLSRRDVV VVASVSCIYG LGTPQSYLDR
     SVELKVGEEV PRDGLLRLLV DVQYTRNDMS FTRGSFRVRG DTVEIIPSYE ELAVRIEFFG
     DEIEALYYLH PLTGEVIRQV DSLRIFPATH YVAGPERMAH AVSAIEEELA ERLAELESQG
     KLLEAQRLRM RTNYDIEMMR QVGFCSGIEN YSRHIDGRGP GTPPATLLDY FPEDFLLVID
     ESHVTVPQIG GMYEGDISRK RNLVEYGFRL PSACDNRPLT WEEFADRIGQ TVYLSATPGP
     YELSQTGGEF VEQVIRPTGL VDPKVVVKPT KGQIDDLIGE IRTRADADQR VLVTTLTKKM
     AEDLTDYLLE MGIRVRYLHS EVDTLRRVEL LRQLRLGDYD VLVGINLLRE GLDLPEVSLV
     AILDADKEGF LRSSRSLIQT IGRAARNVSG EVHMYADKIT DSMREAIDET ERRRAKQIAY
     NEANGIDPQP LRKKIADILD QVYREADDTA VVEVGGSGRN ASRGRRAQGE PGRAVSAGVF
     EGRDTSAMPR AELADLIKDL TAQMMAAARD LQFELAARFR DEIADLKREL RGMDAAGLK
//
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