ID RSFB_MYCTU Reviewed; 122 AA.
AC P9WGE1; F2GFD0; O69655; Q7D525;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Anti-sigma-F factor antagonist RsfB;
DE AltName: Full=Anti-anti-sigma F factor RsfB;
GN Name=rsfB; OrderedLocusNames=Rv3687c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION AS AN ANTI-SIGMA-F FACTOR ANTAGONIST, INTERACTION WITH RSBW,
RP PHOSPHORYLATION AT SER-61, AND MUTAGENESIS OF SER-61.
RX PubMed=12354223; DOI=10.1046/j.1365-2958.2002.03135.x;
RA Beaucher J., Rodrigue S., Jacques P.E., Smith I., Brzezinski R.,
RA Gaudreau L.;
RT "Novel Mycobacterium tuberculosis anti-sigma factor antagonists control
RT sigmaF activity by distinct mechanisms.";
RL Mol. Microbiol. 45:1527-1540(2002).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011445;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Positive regulator of sigma-F (SigF) activity. Binds to anti-
CC sigma-F factor RsbW (UsfX) preventing its binding to SigF, thus
CC activating transcription. {ECO:0000269|PubMed:12354223}.
CC -!- SUBUNIT: Interacts with anti-sigma-F factor RsbW (UsfX). Its
CC phosphorylation may prevent this interaction.
CC {ECO:0000269|PubMed:12354223}.
CC -!- PTM: Putative phosphorylation on Ser-61 may prevent interaction with
CC RsbW. {ECO:0000269|PubMed:12354223}.
CC -!- SIMILARITY: Belongs to the anti-sigma-factor antagonist family.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP46511.1; -; Genomic_DNA.
DR PIR; H70791; H70791.
DR RefSeq; NP_218204.1; NC_000962.3.
DR RefSeq; WP_003419762.1; NZ_NVQJ01000028.1.
DR AlphaFoldDB; P9WGE1; -.
DR SMR; P9WGE1; -.
DR IntAct; P9WGE1; 1.
DR STRING; 83332.Rv3687c; -.
DR iPTMnet; P9WGE1; -.
DR PaxDb; 83332-Rv3687c; -.
DR DNASU; 885599; -.
DR GeneID; 45427684; -.
DR GeneID; 885599; -.
DR KEGG; mtu:Rv3687c; -.
DR TubercuList; Rv3687c; -.
DR eggNOG; COG1366; Bacteria.
DR InParanoid; P9WGE1; -.
DR OrthoDB; 3393696at2; -.
DR PhylomeDB; P9WGE1; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0043856; F:anti-sigma factor antagonist activity; IPI:MTBBASE.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IPI:MTBBASE.
DR CDD; cd07043; STAS_anti-anti-sigma_factors; 1.
DR Gene3D; 3.30.750.24; STAS domain; 1.
DR InterPro; IPR003658; Anti-sigma_ant.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR NCBIfam; TIGR00377; ant_ant_sig; 1.
DR PANTHER; PTHR33495; ANTI-SIGMA FACTOR ANTAGONIST TM_1081-RELATED-RELATED; 1.
DR PANTHER; PTHR33495:SF13; ANTI-SIGMA-F FACTOR ANTAGONIST RSFB; 1.
DR Pfam; PF01740; STAS; 1.
DR SUPFAM; SSF52091; SpoIIaa-like; 1.
DR PROSITE; PS50801; STAS; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..122
FT /note="Anti-sigma-F factor antagonist RsfB"
FT /id="PRO_0000422956"
FT DOMAIN 7..115
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000305|PubMed:12354223"
FT MUTAGEN 61
FT /note="S->A: Still interacts with RsbW."
FT /evidence="ECO:0000269|PubMed:12354223"
FT MUTAGEN 61
FT /note="S->E: No longer interacts with RsbW."
FT /evidence="ECO:0000269|PubMed:12354223"
SQ SEQUENCE 122 AA; 12651 MW; 3F748FC41C6CCBB7 CRC64;
MSAPDSITVT VADHNGVAVL SIGGEIDLIT AAALEEAIGE VVADNPTALV IDLSAVEFLG
SVGLKILAAT SEKIGQSVKF GVVARGSVTR RPIHLMGLDK TFRLFSTLHD ALTGVRGGRI
DR
//
