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Database: UniProt
Entry: P9WH05
LinkDB: P9WH05
Original site: P9WH05 
ID   DEAD_MYCTU              Reviewed;         563 AA.
AC   P9WH05; L0T8U3; Q11039;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   16-OCT-2019, entry version 30.
DE   RecName: Full=ATP-dependent RNA helicase DeaD {ECO:0000255|HAMAP-Rule:MF_00964};
DE            EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_00964};
DE   AltName: Full=Cold-shock DEAD box protein A {ECO:0000255|HAMAP-Rule:MF_00964};
GN   Name=deaD {ECO:0000255|HAMAP-Rule:MF_00964}; Synonyms=csdA;
GN   OrderedLocusNames=Rv1253; ORFNames=MTCY50.29c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M.,
RA   Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III,
RA   Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T.,
RA   Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S.,
RA   Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A.,
RA   Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R.,
RA   Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the
RT   complete genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B.,
RA   Yadav A.K., Shrivastava P., Marimuthu A., Anand S., Sundaram H.,
RA   Kingsbury R., Harsha H.C., Nair B., Prasad T.S., Chauhan D.S.,
RA   Katoch K., Katoch V.M., Kumar P., Chaerkady R., Ramachandran S.,
RA   Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high
RT   resolution mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: DEAD-box RNA helicase involved in various cellular
CC       processes at low temperature, including ribosome biogenesis, mRNA
CC       degradation and translation initiation. {ECO:0000255|HAMAP-
CC       Rule:MF_00964}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00964};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00964}.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DeaD
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00964}.
DR   EMBL; AL123456; CCP44009.1; -; Genomic_DNA.
DR   PIR; E70752; E70752.
DR   RefSeq; NP_215769.1; NC_000962.3.
DR   RefSeq; WP_003898793.1; NZ_NVQJ01000049.1.
DR   SMR; P9WH05; -.
DR   STRING; 83332.Rv1253; -.
DR   PaxDb; P9WH05; -.
DR   PRIDE; P9WH05; -.
DR   EnsemblBacteria; CCP44009; CCP44009; Rv1253.
DR   GeneID; 887069; -.
DR   KEGG; mtu:Rv1253; -.
DR   KEGG; mtv:RVBD_1253; -.
DR   TubercuList; Rv1253; -.
DR   eggNOG; ENOG4105C1J; Bacteria.
DR   eggNOG; COG0513; LUCA.
DR   KO; K05592; -.
DR   OMA; LPQGMPK; -.
DR   PhylomeDB; P9WH05; -.
DR   BioCyc; MTBH37RV:G185E-5424-MONOMER; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005618; C:cell wall; HDA:MTBBASE.
DR   GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0033592; F:RNA strand annealing activity; IBA:GO_Central.
DR   GO; GO:0070417; P:cellular response to cold; IEA:InterPro.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR   GO; GO:0006401; P:RNA catabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd12499; RRM_EcCsdA_like; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   HAMAP; MF_00964; DEAD_helicase_DeaD; 1.
DR   InterPro; IPR034415; CsdA_RRM.
DR   InterPro; IPR005580; DbpA/CsdA_RNA-bd_dom.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR028618; DEAD_helicase_DeaD.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF03880; DbpA; 1.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; RNA-binding; Stress response.
FT   CHAIN         1    563       ATP-dependent RNA helicase DeaD.
FT                                /FTId=PRO_0000055106.
FT   DOMAIN       44    215       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_00964}.
FT   DOMAIN      226    385       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_00964}.
FT   NP_BIND      57     64       ATP. {ECO:0000255|HAMAP-Rule:MF_00964}.
FT   MOTIF        13     41       Q motif.
FT   MOTIF       163    166       DEAD box.
SQ   SEQUENCE   563 AA;  61453 MW;  5D6D0FAE15D3AEDE CRC64;
     MAFPEYSPAA SAATFADLQI HPRVLRAIGD VGYESPTAIQ AATIPALMAG SDVVGLAQTG
     TGKTAAFAIP MLSKIDITSK VPQALVLVPT RELALQVAEA FGRYGAYLSQ LNVLPIYGGS
     SYAVQLAGLR RGAQVVVGTP GRMIDHLERA TLDLSRVDFL VLDEADEMLT MGFADDVERI
     LSETPEYKQV ALFSATMPPA IRKLSAKYLH DPFEVTCKAK TAVAENISQS YIQVARKMDA
     LTRVLEVEPF EAMIVFVRTK QATEEIAEKL RARGFSAAAI SGDVPQAQRE RTITALRDGD
     IDILVATDVA ARGLDVERIS HVLNYDIPHD TESYVHRIGR TGRAGRSGAA LIFVSPRELH
     LLKAIEKATR QTLTEAQLPT VEDVNTQRVA KFADSITNAL GGPGIELFRR LVEEYEREHD
     VPMADIAAAL AVQCRGGEAF LMAPDPPLSR RNRDQRRDRP QRPKRRPDLT TYRVAVGKRH
     KIGPGAIVGA IANEGGLHRS DFGQIRIGPD FSLVELPAKL PRATLKKLAQ TRISGVLIDL
     RPYRPPDAAR RHNGGKPRRK HVG
//
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