ID PGH1_MOUSE Reviewed; 602 AA.
AC P22437;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 27-MAR-2024, entry version 208.
DE RecName: Full=Prostaglandin G/H synthase 1 {ECO:0000305};
DE EC=1.14.99.1 {ECO:0000250|UniProtKB:P23219};
DE AltName: Full=Cyclooxygenase-1;
DE Short=COX-1;
DE AltName: Full=Prostaglandin H2 synthase 1;
DE Short=PGH synthase 1;
DE Short=PGHS-1;
DE Short=PHS 1;
DE AltName: Full=Prostaglandin-endoperoxide synthase 1;
DE Flags: Precursor;
GN Name=Ptgs1 {ECO:0000312|MGI:MGI:97797}; Synonyms=Cox-1, Cox1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2108169; DOI=10.1016/s0021-9258(19)34105-5;
RA Dewitt D.L., El-Harith E.A., Kraemer S.A., Andrews M.J., Yao E.F.,
RA Armstrong R.L., Smith W.L.;
RT "The aspirin and heme-binding sites of ovine and murine prostaglandin
RT endoperoxide synthases.";
RL J. Biol. Chem. 265:5192-5198(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP REVIEW ON FUNCTION; TISSUE SPECIFICITY AND INHIBITION BY NSAIDS.
RX PubMed=10966456; DOI=10.1146/annurev.biochem.69.1.145;
RA Smith W.L., DeWitt D.L., Garavito R.M.;
RT "Cyclooxygenases: structural, cellular, and molecular biology.";
RL Annu. Rev. Biochem. 69:145-182(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP REVIEW ON FUNCTION; INHIBITION BY ASPIRIN AND INVOLVEMENT IN COLORECTAL
RP CANCER.
RX PubMed=24605250; DOI=10.4292/wjgpt.v5.i1.40;
RA Sostres C., Gargallo C.J., Lanas A.;
RT "Aspirin, cyclooxygenase inhibition and colorectal cancer.";
RL World J. Gastrointest. Pharmacol. Ther. 5:40-49(2014).
CC -!- FUNCTION: Dual cyclooxygenase and peroxidase that plays an important
CC role in the biosynthesis pathway of prostanoids, a class of C20
CC oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-
CC eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the
CC inflammatory response. The cyclooxygenase activity oxygenates AA to the
CC hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase
CC activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2
CC (PGH2), the precursor of all 2-series prostaglandins and thromboxanes.
CC This complex transformation is initiated by abstraction of hydrogen at
CC carbon 13 (with S-stereochemistry), followed by insertion of molecular
CC O2 to form the endoperoxide bridge between carbon 9 and 11 that defines
CC prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase
CC activity) yields a hydroperoxy group in PGG2 that is then reduced to
CC PGH2 by two electrons. Involved in the constitutive production of
CC prostanoids in particular in the stomach and platelets. In gastric
CC epithelial cells, it is a key step in the generation of prostaglandins,
CC such as prostaglandin E2 (PGE2), which plays an important role in
CC cytoprotection. In platelets, it is involved in the generation of
CC thromboxane A2 (TXA2), which promotes platelet activation and
CC aggregation, vasoconstriction and proliferation of vascular smooth
CC muscle cells. Can also use linoleate (LA, (9Z,12Z)-octadecadienoate,
CC C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs)
CC in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-
CC (10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-
CC octadecadienoate) its major products. {ECO:0000250|UniProtKB:P05979}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + AH2 + 2 O2 = A + H2O +
CC prostaglandin H2; Xref=Rhea:RHEA:23728, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:32395, ChEBI:CHEBI:57405; EC=1.14.99.1;
CC Evidence={ECO:0000250|UniProtKB:P23219};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:23729;
CC Evidence={ECO:0000250|UniProtKB:P23219};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoate + 2 O2 = prostaglandin G2;
CC Xref=Rhea:RHEA:42596, ChEBI:CHEBI:15379, ChEBI:CHEBI:32395,
CC ChEBI:CHEBI:82629; Evidence={ECO:0000250|UniProtKB:P23219};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42597;
CC Evidence={ECO:0000250|UniProtKB:P23219};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AH2 + prostaglandin G2 = A + H2O + prostaglandin H2;
CC Xref=Rhea:RHEA:42600, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:17499, ChEBI:CHEBI:57405, ChEBI:CHEBI:82629;
CC Evidence={ECO:0000250|UniProtKB:P23219};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42601;
CC Evidence={ECO:0000250|UniProtKB:P23219};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoate + AH2 + O2 = (9R)-hydroxy-(10E,12Z)-
CC octadecadienoate + A + H2O; Xref=Rhea:RHEA:75447, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:77895;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:75448;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoate + AH2 + O2 = (9S)-hydroxy-(10E,12Z)-
CC octadecadienoate + A + H2O; Xref=Rhea:RHEA:75459, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:77852;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:75460;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoate + AH2 + O2 = (13S)-hydroxy-(9Z,11E)-
CC octadecadienoate + A + H2O; Xref=Rhea:RHEA:75451, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:90850;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:75452;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoate + AH2 + O2 = (13R)-hydroxy-(9Z,11E)-
CC octadecadienoate + A + H2O; Xref=Rhea:RHEA:75455, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:30245, ChEBI:CHEBI:136655;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:75456;
CC Evidence={ECO:0000250|UniProtKB:P05979};
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344; Evidence={ECO:0000250};
CC Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
CC {ECO:0000250};
CC -!- ACTIVITY REGULATION: The cyclooxygenase activity is inhibited by
CC nonsteroidal anti-inflammatory drugs (NSAIDs) including ibuprofen,
CC flurbiprofen, ketoprofen, naproxen, flurbiprofen, anirolac, fenclofenac
CC and diclofenac. {ECO:0000250|UniProtKB:P23219}.
CC -!- PATHWAY: Lipid metabolism; prostaglandin biosynthesis.
CC {ECO:0000250|UniProtKB:P23219}.
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Microsome membrane; Peripheral membrane protein.
CC Endoplasmic reticulum membrane; Peripheral membrane protein.
CC -!- MISCELLANEOUS: The conversion of arachidonate to prostaglandin H2 is a
CC 2 step reaction: a cyclooxygenase (COX) reaction which converts
CC arachidonate to prostaglandin G2 (PGG2) and a peroxidase reaction in
CC which PGG2 is reduced to prostaglandin H2 (PGH2). The cyclooxygenase
CC reaction occurs in a hydrophobic channel in the core of the enzyme. The
CC peroxidase reaction occurs at a heme-containing active site located
CC near the protein surface. The nonsteroidal anti-inflammatory drugs
CC (NSAIDs) binding site corresponds to the cyclooxygenase active site.
CC -!- MISCELLANEOUS: Conversion of arachidonate to prostaglandin H2 is
CC mediated by 2 different isozymes: the constitutive PTGS1 and the
CC inducible PTGS2. PTGS1 is expressed constitutively and generally
CC produces prostanoids acutely in response to hormonal stimuli to fine-
CC tune physiological processes requiring instantaneous, continuous
CC regulation (e.g. hemostasis). PTGS2 is inducible and typically produces
CC prostanoids that mediate responses to physiological stresses such as
CC infection and inflammation.
CC -!- MISCELLANEOUS: PTGS1 and PTGS2 are the targets of nonsteroidal anti-
CC inflammatory drugs (NSAIDs) including aspirin and ibuprofen. Aspirin is
CC able to produce an irreversible inactivation of the enzyme through a
CC serine acetylation. Inhibition of the PGHSs with NSAIDs acutely reduces
CC inflammation, pain, and fever, and long-term use of these drugs reduces
CC fatal thrombotic events, as well as the development of colon cancer and
CC Alzheimer's disease. PTGS2 is the principal isozyme responsible for
CC production of inflammatory prostaglandins. New generation PTGSs
CC inhibitors strive to be selective for PTGS2, to avoid side effects such
CC as gastrointestinal complications and ulceration.
CC -!- SIMILARITY: Belongs to the prostaglandin G/H synthase family.
CC {ECO:0000305}.
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DR EMBL; M34141; AAA39913.1; -; mRNA.
DR EMBL; BC005573; AAH05573.1; -; mRNA.
DR CCDS; CCDS15970.1; -.
DR PIR; A35564; A35564.
DR RefSeq; NP_032995.1; NM_008969.4.
DR RefSeq; XP_006497853.1; XM_006497790.3.
DR RefSeq; XP_006497854.1; XM_006497791.3.
DR RefSeq; XP_011237338.1; XM_011239036.2.
DR RefSeq; XP_017171985.1; XM_017316496.1.
DR AlphaFoldDB; P22437; -.
DR SMR; P22437; -.
DR BioGRID; 202462; 5.
DR STRING; 10090.ENSMUSP00000059977; -.
DR BindingDB; P22437; -.
DR ChEMBL; CHEMBL2649; -.
DR DrugCentral; P22437; -.
DR PeroxiBase; 3361; MmPGHS01.
DR GlyCosmos; P22437; 3 sites, No reported glycans.
DR GlyGen; P22437; 3 sites.
DR PhosphoSitePlus; P22437; -.
DR SwissPalm; P22437; -.
DR PaxDb; 10090-ENSMUSP00000059977; -.
DR PeptideAtlas; P22437; -.
DR ProteomicsDB; 287690; -.
DR Pumba; P22437; -.
DR Antibodypedia; 775; 774 antibodies from 45 providers.
DR DNASU; 19224; -.
DR Ensembl; ENSMUST00000062069.6; ENSMUSP00000059977.6; ENSMUSG00000047250.14.
DR GeneID; 19224; -.
DR KEGG; mmu:19224; -.
DR UCSC; uc008jll.2; mouse.
DR AGR; MGI:97797; -.
DR CTD; 5742; -.
DR MGI; MGI:97797; Ptgs1.
DR VEuPathDB; HostDB:ENSMUSG00000047250; -.
DR eggNOG; KOG2408; Eukaryota.
DR GeneTree; ENSGT00390000010743; -.
DR HOGENOM; CLU_022428_0_0_1; -.
DR InParanoid; P22437; -.
DR OMA; LFGSQFQ; -.
DR OrthoDB; 1086441at2759; -.
DR PhylomeDB; P22437; -.
DR TreeFam; TF329675; -.
DR Reactome; R-MMU-140180; COX reactions.
DR Reactome; R-MMU-2162123; Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
DR UniPathway; UPA00662; -.
DR BioGRID-ORCS; 19224; 5 hits in 81 CRISPR screens.
DR ChiTaRS; Ptgs1; mouse.
DR PRO; PR:P22437; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; P22437; Protein.
DR Bgee; ENSMUSG00000047250; Expressed in renal medulla collecting duct and 222 other cell types or tissues.
DR ExpressionAtlas; P22437; baseline and differential.
DR Genevisible; P22437; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:MGI.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IBA:GO_Central.
DR GO; GO:0004601; F:peroxidase activity; IEA:UniProtKB-KW.
DR GO; GO:0004666; F:prostaglandin-endoperoxide synthase activity; ISO:MGI.
DR GO; GO:0019371; P:cyclooxygenase pathway; ISO:MGI.
DR GO; GO:0030216; P:keratinocyte differentiation; NAS:UniProtKB.
DR GO; GO:0007612; P:learning; ISO:MGI.
DR GO; GO:0035633; P:maintenance of blood-brain barrier; ISO:MGI.
DR GO; GO:0007613; P:memory; ISO:MGI.
DR GO; GO:0032811; P:negative regulation of epinephrine secretion; ISO:MGI.
DR GO; GO:0010700; P:negative regulation of norepinephrine secretion; ISO:MGI.
DR GO; GO:0045987; P:positive regulation of smooth muscle contraction; ISO:MGI.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; ISO:MGI.
DR GO; GO:0001516; P:prostaglandin biosynthetic process; IMP:MGI.
DR GO; GO:0006693; P:prostaglandin metabolic process; IMP:MGI.
DR GO; GO:0008217; P:regulation of blood pressure; IMP:MGI.
DR GO; GO:0042127; P:regulation of cell population proliferation; IGI:MGI.
DR GO; GO:0006979; P:response to oxidative stress; IEA:InterPro.
DR CDD; cd00054; EGF_CA; 1.
DR CDD; cd09816; prostaglandin_endoperoxide_synthase; 1.
DR Gene3D; 1.10.640.10; Haem peroxidase domain superfamily, animal type; 1.
DR Gene3D; 2.10.25.10; Laminin; 1.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR019791; Haem_peroxidase_animal.
DR InterPro; IPR010255; Haem_peroxidase_sf.
DR InterPro; IPR037120; Haem_peroxidase_sf_animal.
DR PANTHER; PTHR11903; PROSTAGLANDIN G/H SYNTHASE; 1.
DR PANTHER; PTHR11903:SF6; PROSTAGLANDIN G_H SYNTHASE 1; 1.
DR Pfam; PF03098; An_peroxidase; 1.
DR PRINTS; PR00457; ANPEROXIDASE.
DR SUPFAM; SSF57196; EGF/Laminin; 1.
DR SUPFAM; SSF48113; Heme-dependent peroxidases; 1.
DR PROSITE; PS50026; EGF_3; 1.
DR PROSITE; PS50292; PEROXIDASE_3; 1.
PE 1: Evidence at protein level;
KW Dioxygenase; Disulfide bond; EGF-like domain; Endoplasmic reticulum;
KW Fatty acid biosynthesis; Fatty acid metabolism; Glycoprotein; Heme; Iron;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding; Microsome;
KW Oxidoreductase; Peroxidase; Prostaglandin biosynthesis;
KW Prostaglandin metabolism; Reference proteome; Signal.
FT SIGNAL 1..26
FT CHAIN 27..602
FT /note="Prostaglandin G/H synthase 1"
FT /id="PRO_0000023869"
FT DOMAIN 34..72
FT /note="EGF-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT ACT_SITE 209
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00298"
FT ACT_SITE 387
FT /note="For cyclooxygenase activity"
FT /evidence="ECO:0000250"
FT BINDING 390
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00298"
FT SITE 532
FT /note="Aspirin-acetylated serine"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 146
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 38..49
FT /evidence="ECO:0000250"
FT DISULFID 39..161
FT /evidence="ECO:0000250"
FT DISULFID 43..59
FT /evidence="ECO:0000250"
FT DISULFID 61..71
FT /evidence="ECO:0000250"
FT DISULFID 571..577
FT /evidence="ECO:0000250"
SQ SEQUENCE 602 AA; 69042 MW; 634C0E602045C3A0 CRC64;
MSRRSLSLWF PLLLLLLLPP TPSVLLADPG VPSPVNPCCY YPCQNQGVCV RFGLDNYQCD
CTRTGYSGPN CTIPEIWTWL RNSLRPSPSF THFLLTHGYW LWEFVNATFI REVLMRLVLT
VRSNLIPSPP TYNSAHDYIS WESFSNVSYY TRILPSVPKD CPTPMGTKGK KQLPDVQLLA
QQLLLRREFI PAPQGTNILF AFFAQHFTHQ FFKTSGKMGP GFTKALGHGV DLGHIYGDNL
ERQYHLRLFK DGKLKYQVLD GEVYPPSVEQ ASVLMRYPPG VPPERQMAVG QEVFGLLPGL
MLFSTIWLRE HNRVCDLLKE EHPTWDDEQL FQTTRLILIG ETIKIVIEEY VQHLSGYFLQ
LKFDPELLFR AQFQYRNRIA MEFNHLYHWH PLMPNSFQVG SQEYSYEQFL FNTSMLVDYG
VEALVDAFSR QRAGRIGGGR NFDYHVLHVA VDVIKESREM RLQPFNEYRK RFGLKPYTSF
QELTGEKEMA AELEELYGDI DALEFYPGLL LEKCQPNSIF GESMIEMGAP FSLKGLLGNP
ICSPEYWKPS TFGGDVGFNL VNTASLKKLV CLNTKTCPYV SFRVPDYPGD DGSVLVRRST
EL
//