ID PITX2_CHICK Reviewed; 333 AA.
AC O93385; O73854; Q9PWE0;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 27-MAR-2024, entry version 131.
DE RecName: Full=Pituitary homeobox 2;
DE AltName: Full=Homeobox protein PITX2;
DE Short=cPITX2;
DE AltName: Full=Paired-like homeodomain transcription factor 2;
GN Name=PITX2; Synonyms=PTX2;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=White leghorn;
RX PubMed=9636662; DOI=10.1006/bbrc.1998.8740;
RA St Amand T.R., Ra J., Zhang Y., Hu Y., Baber S.I., Qiu M., Chen Y.;
RT "Cloning and expression pattern of chicken Pitx2: a new component in the
RT SHH signaling pathway controlling embryonic heart looping.";
RL Biochem. Biophys. Res. Commun. 247:100-105(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Ryan A.K., Blumberg B., Rodriguez-Esteban C., Yonei-Tamura S., Tamura K.,
RA Tsukui T., de la Pena J., Sabbagh W., Greenwald J., Choe S., Norris D.P.,
RA Robertson E.J., Evans R.M., Rosenfeld M.G., Izpisua-Belmonte J.-C.;
RT "Ptx2 determines left-right asymmetries in vertebrates.";
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 170-319.
RA Albajar M., Piedra M.E., Icardo J.M., Ros M.A., Rodriguez-Rey J.C.;
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=9347917; DOI=10.1016/s0925-4773(97)00110-x;
RA Kitamura K., Miura H., Yanazawa M., Miyashita T., Kato K.;
RT "Expression patterns of Brx1 (Rieg gene), Sonic hedgehog, Nkx2.2, Dlx1 and
RT Arx during zona limitans intrathalamica and embryonic ventral lateral
RT geniculate nuclear formation.";
RL Mech. Dev. 67:83-96(1997).
CC -!- FUNCTION: May play a role in myoblast differentiation. May be involved
CC in the establishment of left-right asymmetry in the developing embryo
CC (By similarity). May play a role in vasculogenesis during avian
CC embryogenesis. {ECO:0000250|UniProtKB:P97474}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Cytoplasm
CC {ECO:0000250|UniProtKB:P97474}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O93385-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O93385-2; Sequence=VSP_002266, VSP_002267;
CC -!- DEVELOPMENTAL STAGE: At stage HH8, expressed in the mesoderm in the
CC rostral neural plate. At stage HH18, detected in the ventral
CC diencephalon and in Rathke's pouch. First expressed in the zona
CC limitans intrathalamica at stage HH20 until at least 5.5-day-old
CC embryo. {ECO:0000269|PubMed:9347917}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000305}.
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DR EMBL; AF076640; AAC27322.1; -; mRNA.
DR EMBL; AF077092; AAD46097.1; -; mRNA.
DR EMBL; AF063935; AAC18346.1; -; mRNA.
DR PIR; JW0097; JW0097.
DR RefSeq; NP_990341.1; NM_205010.1.
DR AlphaFoldDB; O93385; -.
DR BMRB; O93385; -.
DR SMR; O93385; -.
DR STRING; 9031.ENSGALP00000046615; -.
DR PaxDb; 9031-ENSGALP00000042989; -.
DR GeneID; 395862; -.
DR KEGG; gga:395862; -.
DR VEuPathDB; HostDB:geneid_395862; -.
DR eggNOG; KOG0486; Eukaryota.
DR InParanoid; O93385; -.
DR PhylomeDB; O93385; -.
DR PRO; PR:O93385; -.
DR Proteomes; UP000000539; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0007368; P:determination of left/right symmetry; IEP:BHF-UCL.
DR GO; GO:0030326; P:embryonic limb morphogenesis; IDA:BHF-UCL.
DR GO; GO:0048562; P:embryonic organ morphogenesis; TAS:AgBase.
DR GO; GO:0001947; P:heart looping; IMP:AgBase.
DR GO; GO:0003007; P:heart morphogenesis; IMP:AgBase.
DR GO; GO:0061184; P:positive regulation of dermatome development; IDA:BHF-UCL.
DR GO; GO:0045663; P:positive regulation of myoblast differentiation; IDA:BHF-UCL.
DR GO; GO:2000288; P:positive regulation of myoblast proliferation; IDA:BHF-UCL.
DR GO; GO:2000287; P:positive regulation of myotome development; IDA:BHF-UCL.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:BHF-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0032526; P:response to retinoic acid; IMP:AgBase.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR PANTHER; PTHR45882:SF4; PITUITARY HOMEOBOX 2; 1.
DR PANTHER; PTHR45882; PITUITARY HOMEOBOX HOMOLOG PTX1; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Developmental protein; DNA-binding;
KW Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..333
FT /note="Pituitary homeobox 2"
FT /id="PRO_0000049226"
FT DNA_BIND 101..160
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 295..308
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 301..305
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 16..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..99
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..62
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_002266"
FT VAR_SEQ 63..77
FT /note="LEVHTISDTSSPEAA -> MESNCRKLVSACVQL (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_002267"
FT CONFLICT 234
FT /note="S -> T (in Ref. 3; AAC18346)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="T -> M (in Ref. 3; AAC18346)"
FT /evidence="ECO:0000305"
FT CONFLICT 317
FT /note="N -> K (in Ref. 3; AAC18346)"
FT /evidence="ECO:0000305"
FT CONFLICT 328
FT /note="P -> A (in Ref. 2; AAD46097)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 333 AA; 36558 MW; FE9C68E475F5CCB5 CRC64;
MSCMKDPLSL ERLGAGNNKL CSSSPSSSSS SSSCHHQQPA LAMATALAPG QARSSLEAAK
HRLEVHTISD TSSPEAAEKE KSQQGKSEDA GPEDPSKKKR QRRQRTHFTS QQLQELEATF
QRNRYPDMST REEIAVWTNL TEARVRVWFK NRRAKWRKRE RNQQAELCKN GFGPQFNGLM
QPYDDMYPGY SYNNWAAKGL TSASLSTKSF PFFNSMNVNP LSSQSMFSPP NSISSMSMSS
SMVPSAVTGV PGSGLNSLNN LNNLSNPSLN SAVPTPACPY APPTPPYVYR DTCNSSLASL
RLKAKQHSSF GYASVQNPAS NLSACQYPVD RPV
//
