ID PITX3_DANRE Reviewed; 293 AA.
AC Q6QU75; Q6DUF5;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 158.
DE RecName: Full=Pituitary homeobox 3 {ECO:0000250|UniProtKB:O35160};
DE AltName: Full=Bicoid-like homeodomain transcription factor Pitx3 {ECO:0000312|EMBL:AAT72155.1};
DE AltName: Full=Homeobox protein PITX3;
DE AltName: Full=Paired-like homeodomain transcription factor 3 {ECO:0000312|EMBL:AAR98874.1};
GN Name=pitx3 {ECO:0000312|ZFIN:ZDB-GENE-041229-4}; ORFNames=si:dkey-196H17.1;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAR98874.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:15728669};
RX PubMed=15728669; DOI=10.1242/dev.01723;
RA Dutta S., Dietrich J.E., Aspock G., Burdine R.D., Schier A.,
RA Westerfield M., Varga Z.M.;
RT "pitx3 defines an equivalence domain for lens and anterior pituitary
RT placode.";
RL Development 132:1579-1590(2005).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAT72155.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Eye {ECO:0000269|PubMed:15804565};
RX PubMed=15804565; DOI=10.1016/j.mod.2004.11.012;
RA Shi X., Bosenko D.V., Zinkevich N.S., Foley S., Hyde D.R., Semina E.V.,
RA Vihtelic T.S.;
RT "Zebrafish pitx3 is necessary for normal lens and retinal development.";
RL Mech. Dev. 122:513-527(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [4] {ECO:0000312|EMBL:CAX12174.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye {ECO:0000312|EMBL:AAH94961.1};
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo {ECO:0000269|PubMed:18053265};
RX PubMed=18053265; DOI=10.1186/1471-213x-7-135;
RA Filippi A., Durr K., Ryu S., Willaredt M., Holzschuh J., Driever W.;
RT "Expression and function of nr4a2, lmx1b, and pitx3 in zebrafish
RT dopaminergic and noradrenergic neuronal development.";
RL BMC Dev. Biol. 7:135-135(2007).
CC -!- FUNCTION: Transcriptional regulator which may play a role in the
CC differentiation and maintenance of meso-diencephalic dopaminergic
CC (mdDA) neurons (By similarity). Required for lens and retinal
CC development and for pituitary pre-placode formation and cell
CC specification. {ECO:0000250, ECO:0000269|PubMed:15728669,
CC ECO:0000269|PubMed:15804565, ECO:0000269|PubMed:18053265}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O35160,
CC ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE-
CC ProRule:PRU00138}.
CC -!- TISSUE SPECIFICITY: In the adult, high levels detected in the eye and
CC much lower levels in internal organs such as liver, gastrointestinal
CC system, heart, genitourinary system and pancreas.
CC {ECO:0000269|PubMed:15728669, ECO:0000269|PubMed:15804565,
CC ECO:0000269|PubMed:18053265}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development with low levels
CC detected from 3-8 hpf and highest levels in developing brain and lens
CC at 24 hpf. In the embryo, expressed in anterior neural plate and later
CC in lens and pituitary cells. Detected in the forebrain, lens and
CC pectoral fin buds at 24 hpf. Becomes restricted to the lens equatorial
CC region by 48 hpf. Strongest expression detected in the diencephalon and
CC pituitary at 48 hpf. Evident in the cartilage surrounding the mouth at
CC 72 hpf, appearing in the iris of the eye, developing lower jaw and the
CC branchial arches at 96 hpf and extending to developing musculature
CC along the trunk. During lens development, exhibits widespread
CC expression during the primary differentiation phase of lens formation
CC and then restricted to the region of secondary fiber cell
CC differentiation during the phase of lens growth. Expressed in both
CC proliferating and early differentiating progenitor cells of the
CC posterior tuberculum. {ECO:0000269|PubMed:15728669,
CC ECO:0000269|PubMed:15804565, ECO:0000269|PubMed:18053265}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Bicoid subfamily.
CC {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY525643; AAR98874.1; -; mRNA.
DR EMBL; AY639155; AAT72155.1; -; mRNA.
DR EMBL; AY643793; AAT68296.1; -; Genomic_DNA.
DR EMBL; CR626878; CAX12174.1; -; Genomic_DNA.
DR EMBL; BC094961; AAH94961.1; -; mRNA.
DR RefSeq; NP_991238.1; NM_205675.2.
DR AlphaFoldDB; Q6QU75; -.
DR SMR; Q6QU75; -.
DR STRING; 7955.ENSDARP00000093314; -.
DR PaxDb; 7955-ENSDARP00000093314; -.
DR GeneID; 402974; -.
DR KEGG; dre:402974; -.
DR AGR; ZFIN:ZDB-GENE-041229-4; -.
DR CTD; 5309; -.
DR ZFIN; ZDB-GENE-041229-4; pitx3.
DR eggNOG; KOG0486; Eukaryota.
DR HOGENOM; CLU_030301_0_0_1; -.
DR InParanoid; Q6QU75; -.
DR OMA; HESGCKG; -.
DR OrthoDB; 5395268at2759; -.
DR PhylomeDB; Q6QU75; -.
DR TreeFam; TF351940; -.
DR PRO; PR:Q6QU75; -.
DR Proteomes; UP000000437; Chromosome 13.
DR Bgee; ENSDARG00000070069; Expressed in adenohypophyseal placode and 45 other cell types or tissues.
DR ExpressionAtlas; Q6QU75; baseline.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0021984; P:adenohypophysis development; IMP:ZFIN.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0043010; P:camera-type eye development; IMP:ZFIN.
DR GO; GO:0035270; P:endocrine system development; IMP:ZFIN.
DR GO; GO:0002088; P:lens development in camera-type eye; IMP:ZFIN.
DR GO; GO:0070306; P:lens fiber cell differentiation; IMP:ZFIN.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IMP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR016233; Homeobox_Pitx/unc30.
DR InterPro; IPR003654; OAR_dom.
DR PANTHER; PTHR45882:SF2; PITUITARY HOMEOBOX 3; 1.
DR PANTHER; PTHR45882; PITUITARY HOMEOBOX HOMOLOG PTX1; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF03826; OAR; 1.
DR PIRSF; PIRSF000563; Homeobox_protein_Pitx/Unc30; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; Homeodomain-like; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS50803; OAR; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..293
FT /note="Pituitary homeobox 3"
FT /id="PRO_0000407846"
FT DNA_BIND 60..119
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 255..268
FT /note="OAR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00138"
FT MOTIF 260..264
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:O35160"
FT COMPBIAS 10..31
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 108
FT /note="F -> V (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="R -> L (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="F -> Y (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="P -> A (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="S -> L (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 221
FT /note="N -> S (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="N -> D (in Ref. 2; AAT68296)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 293 AA; 32438 MW; 18F022FB832FBA93 CRC64;
MDFNLLTDSE ARSPALSLSD SGTPQHDPGC KGQDNSDTEK SHQNHTDESN PEDGSLKKKQ
RRQRTHFTSQ QLQELEATFQ RNRYPDMSTR EEIAVWTNLT EARVRVWFKN RRAKWRKRER
NQQAELCKNG FGAQFNGLMQ PYDDMYSGYS YNNWATKSLA SSPLSAKSFP FFNSMNVSPL
SSQPMFSPPS SIPSMNMASS MVPSAVAGVP GSGLNNLGNL NNLNSPTLNS AAVSAAACPY
ATTAGPYMYR DTCNSSLASL RLKAKQHANF AYPAVQNPVS NLSPCQYAVD RPV
//
