ID PKHG2_MOUSE Reviewed; 1340 AA.
AC Q6KAU7; A0PJD9; Q3UNL4; Q6PGK2; Q7TS89; Q8R4H6;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-OCT-2007, sequence version 2.
DT 08-NOV-2023, entry version 116.
DE RecName: Full=Pleckstrin homology domain-containing family G member 2;
DE Short=PH domain-containing family G member 2;
DE AltName: Full=Common site lymphoma/leukemia guanine nucleotide exchange factor;
DE Short=Common site lymphoma/leukemia GEF;
GN Name=Plekhg2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND FUNCTION.
RC STRAIN=BXH2;
RX PubMed=11839748; DOI=10.1074/jbc.m110981200;
RA Himmel K.L., Bi F., Shen H., Jenkins N.A., Copeland N.G., Zheng Y.,
RA Largaespada D.A.;
RT "Activation of clg, a novel dbl family guanine nucleotide exchange factor
RT gene, by proviral insertion at evi24, a common integration site in B cell
RT and myeloid leukemias.";
RL J. Biol. Chem. 277:13463-13472(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryonic tail;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-912.
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: May be a transforming oncogene with exchange activity for
CC CDC42. May be a guanine-nucleotide exchange factor (GEF) for RAC1 and
CC CDC42 (PubMed:11839748). Activated by the binding to subunits beta and
CC gamma of the heterotrimeric guanine nucleotide-binding protein (G
CC protein) (By similarity). Involved in the regulation of actin
CC polymerization (By similarity). {ECO:0000250|UniProtKB:Q9H7P9,
CC ECO:0000269|PubMed:11839748}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6KAU7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6KAU7-2; Sequence=VSP_028531;
CC Name=3;
CC IsoId=Q6KAU7-3; Sequence=VSP_028532;
CC -!- TISSUE SPECIFICITY: Expressed in thymus, skeletal muscle, lung, testis,
CC uterus, pancreas and heart and also expressed during embryogenesis.
CC {ECO:0000269|PubMed:11839748}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH52436.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH56971.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD21360.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF465238; AAL93134.1; -; mRNA.
DR EMBL; AK131110; BAD21360.1; ALT_INIT; mRNA.
DR EMBL; BC025625; AAH25625.1; -; mRNA.
DR EMBL; BC052436; AAH52436.1; ALT_INIT; mRNA.
DR EMBL; BC056971; AAH56971.1; ALT_INIT; mRNA.
DR EMBL; AK144152; BAE25733.1; -; mRNA.
DR CCDS; CCDS52162.1; -. [Q6KAU7-1]
DR RefSeq; NP_001077381.1; NM_001083912.1.
DR RefSeq; NP_001277471.1; NM_001290542.1.
DR RefSeq; NP_620091.2; NM_138752.2.
DR RefSeq; XP_006539504.1; XM_006539441.2.
DR RefSeq; XP_006539505.1; XM_006539442.3.
DR AlphaFoldDB; Q6KAU7; -.
DR SMR; Q6KAU7; -.
DR BioGRID; 221673; 10.
DR IntAct; Q6KAU7; 6.
DR STRING; 10090.ENSMUSP00000092228; -.
DR GlyGen; Q6KAU7; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q6KAU7; -.
DR PhosphoSitePlus; Q6KAU7; -.
DR SwissPalm; Q6KAU7; -.
DR jPOST; Q6KAU7; -.
DR MaxQB; Q6KAU7; -.
DR PaxDb; 10090-ENSMUSP00000092228; -.
DR ProteomicsDB; 288222; -. [Q6KAU7-1]
DR ProteomicsDB; 288223; -. [Q6KAU7-2]
DR ProteomicsDB; 288224; -. [Q6KAU7-3]
DR Pumba; Q6KAU7; -.
DR DNASU; 101497; -.
DR GeneID; 101497; -.
DR KEGG; mmu:101497; -.
DR AGR; MGI:2141874; -.
DR CTD; 64857; -.
DR MGI; MGI:2141874; Plekhg2.
DR eggNOG; KOG3518; Eukaryota.
DR InParanoid; Q6KAU7; -.
DR OrthoDB; 5400510at2759; -.
DR Reactome; R-MMU-193648; NRAGE signals death through JNK.
DR Reactome; R-MMU-416482; G alpha (12/13) signalling events.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR Reactome; R-MMU-9013149; RAC1 GTPase cycle.
DR BioGRID-ORCS; 101497; 5 hits in 78 CRISPR screens.
DR ChiTaRS; Plekhg2; mouse.
DR PRO; PR:Q6KAU7; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q6KAU7; Protein.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IBA:GO_Central.
DR GO; GO:0030833; P:regulation of actin filament polymerization; ISS:UniProtKB.
DR CDD; cd13243; PH_PLEKHG1_G2_G3; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR043324; PH_PLEKHG1_G2_G3.
DR PANTHER; PTHR45924; FI17866P1; 1.
DR PANTHER; PTHR45924:SF3; PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 2; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome; Tumor suppressor.
FT CHAIN 1..1340
FT /note="Pleckstrin homology domain-containing family G
FT member 2"
FT /id="PRO_0000306862"
FT DOMAIN 98..279
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 309..407
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 34..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 684..743
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 820..855
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 907..979
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 991..1028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1047..1069
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1125..1146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1250..1340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 696..714
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 839..853
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 907..921
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 931..979
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1048..1063
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1273..1289
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 441
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT MOD_RES 446
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT MOD_RES 465
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT MOD_RES 1215
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT MOD_RES 1219
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT MOD_RES 1269
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H7P9"
FT VAR_SEQ 1..42
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11839748"
FT /id="VSP_028531"
FT VAR_SEQ 478
FT /note="S -> SGEYPPLEGDPGSQWPGLLTSFFLPP (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_028532"
FT CONFLICT 37
FT /note="A -> AA (in Ref. 3; AAH56971)"
FT /evidence="ECO:0000305"
FT CONFLICT 519
FT /note="L -> P (in Ref. 3; AAH52436/AAH56971 and 4;
FT BAE25733)"
FT /evidence="ECO:0000305"
FT CONFLICT 823..825
FT /note="AET -> TRP (in Ref. 3; AAH25625)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1340 AA; 143376 MW; E4FD7C9382658173 CRC64;
MPEGARGLSL PKPSLRLGCG HQGEVCDCAA VSDTPTAQAA TTMASPRGSG SSTSLSTVGS
EGDPSPACSA SRPEPLPEPP IRLHLLPVGI QGSVKPSRLE RVAREIVETE RAYVRDLRSI
VEDYLGPLMD GRALGLNMEQ VGTLFANIED IYEFSSELLE DLEGCSSAGG IAECFVQRSE
DFDIYTLYCM NYPSSLALLR ELSVSPPATL WLQERQAQLR HSLPLQSFLL KPVQRILKYH
LLLQELGKHW AEGPDSGGRE MVEEAIVSMT AVAWYINDMK RKQEHAARLQ EVQRRLGGWT
GPELSAFGEL VLEGTFRGGG GGGPRLRGGE RLLFLFSRML LVAKRRGPEY TYKGHIFCCN
LSVSETPRDP LGFKVSDLTI PKHRHLFQAK NQEEKRLWIH CLQRLFFENH PASIPAKAKQ
VLLENSLHCA PKSKHIPEPP TSPLDSPRPR DAPGFTPGRR NPAPSPRLSG SRRGRRQSEP
AKEAYVIFPQ NDKPQVKHAG SEGELHPSSE LQPVSASGLP EDLEDAGPPT LDPSGTSITE
EILELLNQRG LRDSGPATHD IPKFPRDSRV PVESEPLPFQ SLPSRESSEE EEEEDLETDE
REPSPLHVLE GLEGSSAAEI PCIPSLTDIP SEVPSLPEIP EAPCLPCLSD ISGVFEVPCL
SPTSTVPDIP SLATTPSFPC GSWLPGPLQE AAQPQATRRE LLSGSNPGRL SESPSESREG
QEDDTEGVSF SAVQREAGTS VQGFPEELEY RSCSEIRSAW QALEQGQLAR PGFPEPLLIL
EDSDLRGGST SGKTGMPHSE RSASRVRELA RLYSERIQQM QRAETRASTN APRRRPRVLA
QPQPSPCPPQ EEAEPGALPA FGHVLVCELA FPLNCTQESV PLGPAVLVQA ATPLCIQGDD
LSGQNLNVSD LSKQGHLSSN SIPPPVPLPG QSNFQNIQVP STSLLPKQEP PDVQVPTAST
LPDTSQLQSQ VPAATPSAGH RNCVEIQVQS TTSLPGQECQ ADTVALSKQE GHEDSQNPNK
APGAEQRDVS IDQGLAVVGG RPVSPLPVCT SSPDQQIPAT TPLPLSTDFP DMEGPGALPL
PTQEGRPDCS IPCNPLPSLS QDVQVPAVIP VSQLQGLTDT RATVPLSSHK QEDAPECLGP
EPSLTDTPAP RLLSSLGQQN TTDGPVSAAA VPLTEQGCSQ DLQGLITSPV QTTMELPKPR
GLVSRVATSE SLDLTPPHSP SLSTRQLLGP SAAALSRYLA ASYISQSLAR RQGPGGEGTV
ASQGHWSSSA PTSRAPSPPP QPQPPAPPAR RLSYATTVSI QVGGGGRLRP AKAQVRLNHP
ALLAAPHPGA VGPSQGPGGS
//
