ID PLSB_MYCLE Reviewed; 775 AA.
AC Q9X7B0;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 27-MAR-2024, entry version 121.
DE RecName: Full=Glycerol-3-phosphate acyltransferase;
DE Short=GPAT;
DE EC=2.3.1.15;
GN Name=plsB; OrderedLocusNames=ML1246; ORFNames=MLCB1610.07;
OS Mycobacterium leprae (strain TN).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=272631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TN;
RX PubMed=11234002; DOI=10.1038/35059006;
RA Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA Barrell B.G.;
RT "Massive gene decay in the leprosy bacillus.";
RL Nature 409:1007-1011(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl-CoA + sn-glycerol 3-phosphate = a 1-acyl-sn-glycero-3-
CC phosphate + CoA; Xref=Rhea:RHEA:15325, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57597, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342; EC=2.3.1.15;
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GPAT/DAPAT family. {ECO:0000305}.
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DR EMBL; AL049913; CAB43153.1; -; Genomic_DNA.
DR EMBL; AL583921; CAC31627.1; -; Genomic_DNA.
DR PIR; T45238; T45238.
DR RefSeq; NP_301900.1; NC_002677.1.
DR RefSeq; WP_010908221.1; NC_002677.1.
DR AlphaFoldDB; Q9X7B0; -.
DR SMR; Q9X7B0; -.
DR STRING; 272631.gene:17575078; -.
DR KEGG; mle:ML1246; -.
DR PATRIC; fig|272631.5.peg.2298; -.
DR Leproma; ML1246; -.
DR eggNOG; COG2937; Bacteria.
DR HOGENOM; CLU_015407_1_0_11; -.
DR OrthoDB; 335193at2; -.
DR UniPathway; UPA00557; UER00612.
DR Proteomes; UP000000806; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004366; F:glycerol-3-phosphate O-acyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0102420; F:sn-1-glycerol-3-phosphate C16:0-DCA-CoA acyl transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd07993; LPLAT_DHAPAT-like; 1.
DR HAMAP; MF_00393; Glyc3P_acyltrans; 1.
DR InterPro; IPR022284; GPAT/DHAPAT.
DR InterPro; IPR045520; GPAT/DHAPAT_C.
DR InterPro; IPR041728; GPAT/DHAPAT_LPLAT.
DR InterPro; IPR028354; GPAT_PlsB.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR PANTHER; PTHR12563:SF17; DIHYDROXYACETONE PHOSPHATE ACYLTRANSFERASE; 1.
DR PANTHER; PTHR12563; GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR Pfam; PF19277; GPAT_C; 1.
DR PIRSF; PIRSF500064; GPAT; 1.
DR PIRSF; PIRSF000437; GPAT_DHAPAT; 1.
DR SMART; SM00563; PlsC; 1.
DR SUPFAM; SSF69593; Glycerol-3-phosphate (1)-acyltransferase; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cell membrane; Lipid biosynthesis; Lipid metabolism;
KW Membrane; Phospholipid biosynthesis; Phospholipid metabolism;
KW Reference proteome; Transferase.
FT CHAIN 1..775
FT /note="Glycerol-3-phosphate acyltransferase"
FT /id="PRO_0000195224"
FT MOTIF 268..273
FT /note="HXXXXD motif"
SQ SEQUENCE 775 AA; 87364 MW; 03DD77C778293CDF CRC64;
MTEPDVEISS VLTGEDTLVL ASMDTPAEIE LVMDWLCQQR NRNPDIKFDV LKLPSRNLAP
AALTALVEQL ESDEDRSVVP VRVFWMPPAE RSKLAKLAGL LPGRDPYHPN RRQQRHILKT
DARRALVIAG DSAKVSELRQ YWRDTTVGEN ECDFAQFVTR RAILAMERAE SRILGPQYKS
PRLVKPEILA STRFRAGLEK ISGATVEEAG KMLDELATGW SRASVDLVSV LGRMLSRGFE
PEIDYDEYQV AAMRAALEAH PAVLLFSHRS YIDGAVVPVA MQENRLPPVH VFAGINLSFG
LMGPLLRRSG VIFIRRNIGD NPLYKYVLRE YVGYIVEKRF NLSWSIEGTR SRTGKMLPPK
LGLLTYVADA YLDGRSEDIL LQPVSISFDQ LHETAEYAAY ARGGEKTPEG VAWLYSFIKA
QGERNYGKIY VRFPEAVSMR QYLGAPHGAL VQDQDAKRLA LQKMSFEVAW RILCATPVTA
TALVSALLLT TRGVALTLDQ LHHTLQESLD YLERKQTPVS KSALRLRSRE GVRAAVDALS
SGHPITRVDS GREPVWYITP GNEHAAAFYR NSVIHAFLET SIVELALAHA RHVEGDRMKV
FWAQAMRLRD LLKFDFYFAD SAAFRANIAE EIAWHQNWED RVSGDGDDID AMLLTKRPLI
SDAMLRVFFE AYDIVADVLR DAPADVGQKE LTELALGVGR QYVAQGRVRS GESVSTLLFA
TAYQVVVDQN LIAPAPDLAE RRMVFRRELR DIRRDFDYVE QIARSRFIVR EFKSR
//
