ID PP272_ARATH Reviewed; 850 AA.
AC Q5G1T1; Q9M3B5;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=Pentatricopeptide repeat-containing protein At3g49170, chloroplastic;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2261;
DE Flags: Precursor;
GN Name=EMB2261; Synonyms=PCMP-H78; OrderedLocusNames=At3g49170;
GN ORFNames=F2K15.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND FUNCTION.
RX PubMed=15647901; DOI=10.1007/s00425-004-1452-x;
RA Cushing D.A., Forsthoefel N.R., Gestaut D.R., Vernon D.M.;
RT "Arabidopsis emb175 and other ppr knockout mutants reveal essential roles
RT for pentatricopeptide repeat (PPR) proteins in plant embryogenesis.";
RL Planta 221:424-436(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
CC -!- FUNCTION: May play a role in embryogenesis.
CC {ECO:0000269|PubMed:15647901}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB66396.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AY864347; AAW62962.1; -; mRNA.
DR EMBL; AY864348; AAW62963.1; -; Genomic_DNA.
DR EMBL; AL132956; CAB66396.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78507.1; -; Genomic_DNA.
DR PIR; T45822; T45822.
DR RefSeq; NP_190486.2; NM_114776.4.
DR AlphaFoldDB; Q5G1T1; -.
DR SMR; Q5G1T1; -.
DR STRING; 3702.Q5G1T1; -.
DR iPTMnet; Q5G1T1; -.
DR PaxDb; 3702-AT3G49170-1; -.
DR ProteomicsDB; 249194; -.
DR EnsemblPlants; AT3G49170.1; AT3G49170.1; AT3G49170.
DR GeneID; 824078; -.
DR Gramene; AT3G49170.1; AT3G49170.1; AT3G49170.
DR KEGG; ath:AT3G49170; -.
DR Araport; AT3G49170; -.
DR TAIR; AT3G49170; EMB2261.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_15_0_1; -.
DR InParanoid; Q5G1T1; -.
DR OMA; CIRSRNF; -.
DR OrthoDB; 447472at2759; -.
DR PhylomeDB; Q5G1T1; -.
DR PRO; PR:Q5G1T1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q5G1T1; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009451; P:RNA modification; IEA:InterPro.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 6.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR046848; E_motif.
DR InterPro; IPR046849; Eplus_motif.
DR InterPro; IPR002885; Pentatricopeptide_rpt.
DR InterPro; IPR045215; PPR_prot_At1g15510.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR NCBIfam; TIGR00756; PPR; 6.
DR PANTHER; PTHR24015; OS07G0578800 PROTEIN-RELATED; 1.
DR PANTHER; PTHR24015:SF2006; PPR_LONG DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF20431; E_motif; 1.
DR Pfam; PF20430; Eplus_motif; 1.
DR Pfam; PF01535; PPR; 2.
DR Pfam; PF13041; PPR_2; 5.
DR SUPFAM; SSF48452; TPR-like; 1.
DR PROSITE; PS51375; PPR; 18.
PE 2: Evidence at transcript level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 51..850
FT /note="Pentatricopeptide repeat-containing protein
FT At3g49170, chloroplastic"
FT /id="PRO_0000356131"
FT REPEAT 61..95
FT /note="PPR 1"
FT REPEAT 96..130
FT /note="PPR 2"
FT REPEAT 131..164
FT /note="PPR 3"
FT REPEAT 165..199
FT /note="PPR 4"
FT REPEAT 201..232
FT /note="PPR 5"
FT REPEAT 233..267
FT /note="PPR 6"
FT REPEAT 268..302
FT /note="PPR 7"
FT REPEAT 303..334
FT /note="PPR 8"
FT REPEAT 335..370
FT /note="PPR 9"
FT REPEAT 372..406
FT /note="PPR 10"
FT REPEAT 407..437
FT /note="PPR 11"
FT REPEAT 438..472
FT /note="PPR 12"
FT REPEAT 473..507
FT /note="PPR 13"
FT REPEAT 508..538
FT /note="PPR 14"
FT REPEAT 539..573
FT /note="PPR 15"
FT REPEAT 574..609
FT /note="PPR 16"
FT REPEAT 610..640
FT /note="PPR 17"
FT REGION 645..720
FT /note="Type E motif"
FT REGION 721..751
FT /note="Type E(+) motif"
FT REGION 752..850
FT /note="Type DYW motif"
SQ SEQUENCE 850 AA; 95496 MW; 823526AE58D293E2 CRC64;
MAMISFSFPS PAKLPIKSQP SVSNRINVAD RLILRHLNAG DLRGAVSALD LMARDGIRPM
DSVTFSSLLK SCIRARDFRL GKLVHARLIE FDIEPDSVLY NSLISLYSKS GDSAKAEDVF
ETMRRFGKRD VVSWSAMMAC YGNNGRELDA IKVFVEFLEL GLVPNDYCYT AVIRACSNSD
FVGVGRVTLG FLMKTGHFES DVCVGCSLID MFVKGENSFE NAYKVFDKMS ELNVVTWTLM
ITRCMQMGFP REAIRFFLDM VLSGFESDKF TLSSVFSACA ELENLSLGKQ LHSWAIRSGL
VDDVECSLVD MYAKCSADGS VDDCRKVFDR MEDHSVMSWT ALITGYMKNC NLATEAINLF
SEMITQGHVE PNHFTFSSAF KACGNLSDPR VGKQVLGQAF KRGLASNSSV ANSVISMFVK
SDRMEDAQRA FESLSEKNLV SYNTFLDGTC RNLNFEQAFK LLSEITEREL GVSAFTFASL
LSGVANVGSI RKGEQIHSQV VKLGLSCNQP VCNALISMYS KCGSIDTASR VFNFMENRNV
ISWTSMITGF AKHGFAIRVL ETFNQMIEEG VKPNEVTYVA ILSACSHVGL VSEGWRHFNS
MYEDHKIKPK MEHYACMVDL LCRAGLLTDA FEFINTMPFQ ADVLVWRTFL GACRVHSNTE
LGKLAARKIL ELDPNEPAAY IQLSNIYACA GKWEESTEMR RKMKERNLVK EGGCSWIEVG
DKIHKFYVGD TAHPNAHQIY DELDRLITEI KRCGYVPDTD LVLHKLEEEN DEAEKERLLY
QHSEKIAVAF GLISTSKSRP VRVFKNLRVC GDCHNAMKYI STVSGREIVL RDLNRFHHFK
DGKCSCNDYW
//
