ID PRIS_PICTO Reviewed; 370 AA.
AC Q6L0W5;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 97.
DE RecName: Full=DNA primase small subunit PriS {ECO:0000255|HAMAP-Rule:MF_00700};
DE EC=2.7.7.- {ECO:0000255|HAMAP-Rule:MF_00700};
GN Name=priS {ECO:0000255|HAMAP-Rule:MF_00700}; Synonyms=priA;
GN OrderedLocusNames=PTO0802;
OS Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC
OS 100828 / KAW 2/3).
OC Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales;
OC Picrophilaceae; Picrophilus.
OX NCBI_TaxID=1122961;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828 / KAW 2/3;
RX PubMed=15184674; DOI=10.1073/pnas.0401356101;
RA Fuetterer O., Angelov A., Liesegang H., Gottschalk G., Schleper C.,
RA Schepers B., Dock C., Antranikian G., Liebl W.;
RT "Genome sequence of Picrophilus torridus and its implications for life
RT around pH 0.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9091-9096(2004).
CC -!- FUNCTION: Catalytic subunit of DNA primase, an RNA polymerase that
CC catalyzes the synthesis of short RNA molecules used as primers for DNA
CC polymerase during DNA replication. The small subunit contains the
CC primase catalytic core and has DNA synthesis activity on its own.
CC Binding to the large subunit stabilizes and modulates the activity,
CC increasing the rate of DNA synthesis while decreasing the length of the
CC DNA fragments, and conferring RNA synthesis capability. The DNA
CC polymerase activity may enable DNA primase to also catalyze primer
CC extension after primer synthesis. May also play a role in DNA repair.
CC {ECO:0000255|HAMAP-Rule:MF_00700}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00700};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00700};
CC -!- SUBUNIT: Heterodimer of a small subunit (PriS) and a large subunit
CC (PriL). {ECO:0000255|HAMAP-Rule:MF_00700}.
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase small subunit
CC family. {ECO:0000255|HAMAP-Rule:MF_00700}.
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DR EMBL; AE017261; AAT43387.1; -; Genomic_DNA.
DR RefSeq; WP_011177603.1; NC_005877.1.
DR AlphaFoldDB; Q6L0W5; -.
DR SMR; Q6L0W5; -.
DR STRING; 263820.PTO0802; -.
DR PaxDb; 263820-PTO0802; -.
DR GeneID; 2844074; -.
DR KEGG; pto:PTO0802; -.
DR PATRIC; fig|263820.9.peg.837; -.
DR eggNOG; arCOG04110; Archaea.
DR HOGENOM; CLU_056123_1_0_2; -.
DR InParanoid; Q6L0W5; -.
DR OrthoDB; 31125at2157; -.
DR Proteomes; UP000000438; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd04860; AE_Prim_S; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR HAMAP; MF_00700; DNA_primase_sml_arc; 1.
DR InterPro; IPR002755; DNA_primase_S.
DR InterPro; IPR014052; DNA_primase_ssu_euk/arc.
DR InterPro; IPR023639; DNA_primase_ssu_PriS.
DR NCBIfam; TIGR00335; primase_sml; 1.
DR PANTHER; PTHR10536; DNA PRIMASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR10536:SF0; DNA PRIMASE SMALL SUBUNIT; 1.
DR Pfam; PF01896; DNA_primase_S; 1.
DR SUPFAM; SSF56747; Prim-pol domain; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-directed RNA polymerase; Magnesium; Manganese;
KW Metal-binding; Nucleotidyltransferase; Primosome; Transcription;
KW Transferase.
FT CHAIN 1..370
FT /note="DNA primase small subunit PriS"
FT /id="PRO_0000046747"
FT ACT_SITE 92
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00700"
FT ACT_SITE 94
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00700"
FT ACT_SITE 272
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00700"
SQ SEQUENCE 370 AA; 43752 MW; 74EF0810ABEE8477 CRC64;
MISYDKSLNY FSLYYRSNHL IMPDLISKRE IGYIPFSGTM IRHLSFRNHR EIESFVKRNT
PRHLYYSSAY YIKPDEKRME KKIWEGAELI FDLDADHIPG SDKMTYEEIL LEVKKHVSRL
LNYLINDFGF DDDSIKLYFS GGRGYHVHVV SDRVYSLDSD ARREIGNYIK MEDFTIDNII
RASREKPESG PLKRFNEYIS EIYSDENYLK RFYNGDFDRY YKSLDVYKDG KKKIDIMREN
NYEKFKIVSK RDLDVLNNIL NDFKDKYSAE IDEPVTTDVH RLIRFPGSLH GKTGLAVTPV
NINEFDNFDP LISAVPEVFK DKYEHVYLNS DYMITMMNEK FSLNAGENKV PLYLALFLTG
MKIGNFIEKK
//
