ID PSBW_ARATH Reviewed; 133 AA.
AC Q39194; O04338;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 27-MAR-2024, entry version 118.
DE RecName: Full=Photosystem II reaction center W protein, chloroplastic;
DE AltName: Full=PSII 6.1 kDa protein;
DE Flags: Precursor;
GN Name=PSBW; OrderedLocusNames=At2g30570; ORFNames=T6B20.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Landsberg erecta; TISSUE=Seedling;
RA Lorkovic Z.J., Schroeder W.P., Pakrasi H.B., Irrgang K.-D., Herrmann R.G.,
RA Oelmueller R.;
RT "Characterization of PsbW, the only nuclear-encoded component of the
RT photosystem II reaction center.";
RL Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=10950961; DOI=10.1074/jbc.m006300200;
RA Shi L.-X., Lorkovic Z.J., Oelmueller R., Schroeder W.P.;
RT "The low molecular mass PsbW protein is involved in the stabilization of
RT the dimeric photosystem II complex in Arabidopsis thaliana.";
RL J. Biol. Chem. 275:37945-37950(2000).
CC -!- FUNCTION: Stabilizes dimeric photosystem II (PSII). In its absence no
CC dimeric PSII accumulates and there is a reduction of monomeric PSII.
CC {ECO:0000269|PubMed:10950961}.
CC -!- SUBUNIT: Part of the photosystem II complex. PSII is composed of 1 copy
CC each of membrane proteins PsbA, PsbB, PsbC, PsbD, numerous small
CC proteins, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes. Found in
CC monomeric, dimeric and in etioplastic PSII (i.e. before PSII is fully
CC assembled and functional). {ECO:0000250|UniProtKB:Q41387}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-
CC pass membrane protein. Note=The N-terminus is found within the
CC thylakoid lumen (By similarity). {ECO:0000250|UniProtKB:Q41387}.
CC -!- DISRUPTION PHENOTYPE: Plants have no obvious physical phenotype.
CC However, there is no dimeric photosystem II (PSII) and there is only a
CC 50% rate of normal oxygen evolution. There is an approximately 40%
CC decrease in the amount of PSII and the oxygen evolving complex in these
CC plants. The monomeric PSII that remains functions normally with respect
CC to electron transfer. {ECO:0000269|PubMed:10950961}.
CC -!- SIMILARITY: Belongs to the psbW family. {ECO:0000305}.
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DR EMBL; X90769; CAA62296.1; -; Genomic_DNA.
DR EMBL; U93215; AAB63080.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08409.1; -; Genomic_DNA.
DR EMBL; AF325042; AAG40394.1; -; mRNA.
DR EMBL; AF375448; AAK53032.1; -; mRNA.
DR EMBL; AY143958; AAN28897.1; -; mRNA.
DR EMBL; AY087402; AAM64951.1; -; mRNA.
DR PIR; A84710; A84710.
DR PIR; S60662; S60662.
DR RefSeq; NP_180615.1; NM_128609.4.
DR PDB; 5MDX; EM; 5.30 A; W/w=80-133.
DR PDB; 7OUI; EM; 2.79 A; W/w=80-133.
DR PDBsum; 5MDX; -.
DR PDBsum; 7OUI; -.
DR AlphaFoldDB; Q39194; -.
DR EMDB; EMD-13078; -.
DR EMDB; EMD-3491; -.
DR SMR; Q39194; -.
DR BioGRID; 2955; 1.
DR IntAct; Q39194; 1.
DR STRING; 3702.Q39194; -.
DR TCDB; 3.E.2.2.3; the photosynthetic reaction center (prc) family.
DR PaxDb; 3702-AT2G30570-1; -.
DR EnsemblPlants; AT2G30570.1; AT2G30570.1; AT2G30570.
DR GeneID; 817606; -.
DR Gramene; AT2G30570.1; AT2G30570.1; AT2G30570.
DR KEGG; ath:AT2G30570; -.
DR Araport; AT2G30570; -.
DR TAIR; AT2G30570; PSBW.
DR eggNOG; ENOG502S50E; Eukaryota.
DR HOGENOM; CLU_156148_0_0_1; -.
DR InParanoid; Q39194; -.
DR OMA; KGGKVKC; -.
DR OrthoDB; 297463at2759; -.
DR PhylomeDB; Q39194; -.
DR BioCyc; MetaCyc:AT2G30570-MONOMER; -.
DR PRO; PR:Q39194; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q39194; baseline and differential.
DR Genevisible; Q39194; AT.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR GO; GO:0042549; P:photosystem II stabilization; IMP:TAIR.
DR InterPro; IPR009806; PSII_PsbW_class2.
DR PANTHER; PTHR34552; PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC; 1.
DR PANTHER; PTHR34552:SF12; PHOTOSYSTEM II REACTION CENTER W PROTEIN, CHLOROPLASTIC; 1.
DR Pfam; PF07123; PsbW; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Photosynthesis; Photosystem II;
KW Plastid; Reference proteome; Thylakoid; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 54..79
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN 80..133
FT /note="Photosystem II reaction center W protein,
FT chloroplastic"
FT /id="PRO_0000271554"
FT TOPO_DOM 80..99
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000250"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..133
FT /note="Stromal"
FT /evidence="ECO:0000250"
FT CONFLICT 118
FT /note="V -> A (in Ref. 1; CAA62296)"
FT /evidence="ECO:0000305"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:7OUI"
FT HELIX 99..120
FT /evidence="ECO:0007829|PDB:7OUI"
SQ SEQUENCE 133 AA; 13678 MW; 31CBA2DB63E10B74 CRC64;
MASFTASAST VSAARPALLL KPTVAISAPV LGLPPMGKKK GGVRCSMETK QGNVSVMGAG
VSAAATAALT AVMSNPAMAL VDERMSTEGT GLPFGLSNNL LGWILFGVFG LIWTFFFVYT
SSLEEDEESG LSL
//
