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Database: UniProt
Entry: Q03942
LinkDB: Q03942
Original site: Q03942 
ID   RKM2_YEAST              Reviewed;         479 AA.
AC   Q03942; D6VSI1;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   10-APR-2019, entry version 111.
DE   RecName: Full=Ribosomal lysine N-methyltransferase 2 {ECO:0000303|PubMed:17005568};
DE            EC=2.1.1.- {ECO:0000269|PubMed:17005568};
GN   Name=RKM2 {ECO:0000303|PubMed:17005568};
GN   OrderedLocusNames=YDR198C {ECO:0000312|SGD:S000002606};
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA   Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA   Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA   Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA   Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA   Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA   Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA   Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA   Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA   Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA   Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA   Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA   Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA   Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA   Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA   Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA   Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA   Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA   Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA   Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA   Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA   Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [4]
RP   FUNCTION.
RX   PubMed=17005568; DOI=10.1074/jbc.M606578200;
RA   Porras-Yakushi T.R., Whitelegge J.P., Clarke S.;
RT   "A novel SET domain methyltransferase in yeast: Rkm2-dependent
RT   trimethylation of ribosomal protein L12ab at lysine 10.";
RL   J. Biol. Chem. 281:35835-35845(2006).
RN   [5]
RP   FUNCTION.
RX   PubMed=18957409; DOI=10.1074/jbc.M806006200;
RA   Webb K.J., Laganowsky A., Whitelegge J.P., Clarke S.G.;
RT   "Identification of two SET domain proteins required for methylation of
RT   lysine residues in yeast ribosomal protein Rpl42ab.";
RL   J. Biol. Chem. 283:35561-35568(2008).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent protein-lysine N-
CC       methyltransferase that trimethylates 60S ribosomal protein L12
CC       (RPL12A and RPL12B) at 'Lys-4' and 'Lys-11'.
CC       {ECO:0000269|PubMed:17005568, ECO:0000269|PubMed:18957409}.
CC   -!- MISCELLANEOUS: Present with 768 molecules/cell in log phase SD
CC       medium. {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the class V-like SAM-binding
CC       methyltransferase superfamily. RKM2 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00190, ECO:0000305}.
DR   EMBL; Z48784; CAA88711.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA12041.1; -; Genomic_DNA.
DR   PIR; S52705; S52705.
DR   RefSeq; NP_010484.1; NM_001180506.1.
DR   ProteinModelPortal; Q03942; -.
DR   BioGrid; 32250; 54.
DR   DIP; DIP-6397N; -.
DR   IntAct; Q03942; 3.
DR   STRING; 4932.YDR198C; -.
DR   MaxQB; Q03942; -.
DR   PaxDb; Q03942; -.
DR   PRIDE; Q03942; -.
DR   EnsemblFungi; YDR198C_mRNA; YDR198C_mRNA; YDR198C.
DR   GeneID; 851779; -.
DR   KEGG; sce:YDR198C; -.
DR   EuPathDB; FungiDB:YDR198C; -.
DR   SGD; S000002606; RKM2.
DR   GeneTree; ENSGT00940000153577; -.
DR   HOGENOM; HOG000001042; -.
DR   InParanoid; Q03942; -.
DR   OMA; INSRCLY; -.
DR   BioCyc; YEAST:G3O-29784-MONOMER; -.
DR   PRO; PR:Q03942; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   GO; GO:0016279; F:protein-lysine N-methyltransferase activity; IDA:SGD.
DR   GO; GO:0018026; P:peptidyl-lysine monomethylation; IDA:SGD.
DR   GO; GO:0018023; P:peptidyl-lysine trimethylation; IMP:SGD.
DR   InterPro; IPR001214; SET_dom.
DR   InterPro; IPR016852; SET_MeTrfase.
DR   PIRSF; PIRSF027158; Lys_MTase_YDR198C_prd; 1.
DR   PROSITE; PS50280; SET; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    479       Ribosomal lysine N-methyltransferase 2.
FT                                /FTId=PRO_0000253809.
FT   DOMAIN       22    325       SET. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00190}.
FT   BINDING     324    324       S-adenosyl-L-methionine.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00190}.
SQ   SEQUENCE   479 AA;  55950 MW;  4E19ADFADEE1A0A4 CRC64;
     MEGKVDVLLT WLKKSDKFYI APNISICESP ETGRGIVLSH GSIRKNDIIV SVPSSKQLNF
     HTILYHISKF NKELNIPGIT IDRKPINYED NIIEAENKAW ADPRYGLYSE LSKEFLLSLS
     SFQLVSFYIL VENFLLPKWT HNEIYSDWKP FFDVWPSMEE LRSIPAIWNC DPNSRYHSLI
     EYLPAASRKH MARISGLVRE DWETISEVVL KWNEIYGSLS CTKNSDKFTS DELFSLFVHV
     YFIINSRCLY AKIPLKIEDS PSNFTLVPYV DFMNHICESD LHCYPQLSPQ LRSEGENIIG
     IGQFTIRCGD HLYDNINEEL FLNYGAHSND FLLNEYGFVV DGNKWNYLDI SDEIIELIDD
     DKKEVKTFLL EHDYWGDYTI NETDISYRIF VALNYYVTRD ERRVRKFIEG YISEDYFKPK
     ISSVLKELLV SLTAKYTKTL SELTEKVSNL ENNLCLQNLI TIYKGYIKIL TQHLQDLQS
//
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