ID CHVG_AGRFC Reviewed; 596 AA.
AC Q07737;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 27-MAR-2024, entry version 148.
DE RecName: Full=Sensor protein ChvG;
DE EC=2.7.13.3;
GN Name=chvG; OrderedLocusNames=Atu0033; ORFNames=AGR_C_52;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8407839; DOI=10.1128/jb.175.20.6614-6625.1993;
RA Charles T.C., Nester E.W.;
RT "A chromosomally encoded two-component sensory transduction system is
RT required for virulence of Agrobacterium tumefaciens.";
RL J. Bacteriol. 175:6614-6625(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Member of a two-component regulatory system ChvG/ChvI.
CC Activates ChvI by phosphorylation (Potential). {ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA03552.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L18860; AAA03552.1; ALT_FRAME; Unassigned_DNA.
DR EMBL; AE007869; AAK85857.1; -; Genomic_DNA.
DR PIR; A49902; A49902.
DR PIR; AB2581; AB2581.
DR PIR; H97362; H97362.
DR RefSeq; NP_353072.1; NC_003062.2.
DR RefSeq; WP_006311105.1; NC_003062.2.
DR AlphaFoldDB; Q07737; -.
DR SMR; Q07737; -.
DR STRING; 176299.Atu0033; -.
DR EnsemblBacteria; AAK85857; AAK85857; Atu0033.
DR GeneID; 66223049; -.
DR KEGG; atu:Atu0033; -.
DR PATRIC; fig|176299.10.peg.33; -.
DR eggNOG; COG0642; Bacteria.
DR HOGENOM; CLU_000445_89_6_5; -.
DR OrthoDB; 9805942at2; -.
DR PhylomeDB; Q07737; -.
DR BioCyc; AGRO:ATU0033-MONOMER; -.
DR BRENDA; 2.7.13.3; 200.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR CDD; cd06225; HAMP; 1.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 1.10.287.130; -; 1.
DR Gene3D; 6.10.340.10; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR025908; Sensor_TM1.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR025919; Stimulus_sens_dom.
DR PANTHER; PTHR45436:SF5; SENSOR HISTIDINE KINASE TRCS; 1.
DR PANTHER; PTHR45436; SENSOR HISTIDINE KINASE YKOH; 1.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF13755; Sensor_TM1; 1.
DR Pfam; PF13756; Stimulus_sens_1; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF47384; Homodimeric domain of signal transducing histidine kinase; 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase;
KW Transmembrane; Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..596
FT /note="Sensor protein ChvG"
FT /id="PRO_0000074720"
FT TOPO_DOM 1..47
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..283
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..596
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 301..356
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 364..592
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 367
FT /note="Phosphohistidine"
FT /evidence="ECO:0000250"
FT CONFLICT 94
FT /note="A -> R (in Ref. 1; AAA03552)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="R -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 314..318
FT /note="AIRVR -> RSGA (in Ref. 1; AAA03552)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 596 AA; 65722 MW; 8931990E49736C4B CRC64;
MLKKTPETVS DSDDAEERGS ERRHRIHPLT IIRRIFGNAV FSSLTRRILF FNVAATVVLV
GGILYLNQFR EGLIDARVES LLTQGEIIAG AVSASASVDT NSITINPEKL LELQAGQSIT
PAPNDEDLSF PINPERVAPV LRRLISPTRT RARLFDADAN LLLDSRHLYS RGQVLRFDLP
PVTPETQTWG DWFTSMFNRM LQPSSLPQYK EAPGGDGSIY PEVMNALTGV RGAVVRVTEK
GELIVSVAVP VQRFRAVLGV LLLSTQAGDI DKIVHAERLA IMRVFGIATL VNIVLSLLLS
STIATPLRRL SAAAIRVRRG ARTREEIPDF SARQDEIGNL SIALREMTTA LYDRIDAIES
FAADVSHELK NPLTSLRSAV ETLPRAKTEE SKQRLTEIIF HDVRRLDRLI SDISDASRLD
AELARADASP LDLDVLMKGL VDISRQISTK KKSVAIDYVA DRKAGAKTSF VVNGHDLRIG
QIVTNLIENA RSFVSEESGR ITVRLSRHKD RCIVQVEDNG PGIQAEDIDR IFERFYTDRP
ASEGFGQNSG LGLSISRQIA EAHGGSLRAE NVVDKYGVIS GARFTLSLPA AETHER
//
