GenomeNet

Database: UniProt
Entry: Q07886
LinkDB: Q07886
Original site: Q07886 
ID   DDX49_DROME             Reviewed;         521 AA.
AC   Q07886; Q9V528;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   16-OCT-2019, entry version 158.
DE   RecName: Full=Probable ATP-dependent RNA helicase Dbp45A;
DE            Short=DEAD box protein 45A;
DE            EC=3.6.4.13;
GN   Name=Dbp45A; ORFNames=CG12759;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=7692973; DOI=10.1016/0167-4781(93)90052-f;
RA   Lavoie C.A., Harvey M., Lasko P.F.;
RT   "Dbp45A encodes a Drosophila DEAD box protein with similarity to a
RT   putative yeast helicase involved in ribosome assembly.";
RL   Biochim. Biophys. Acta 1216:140-144(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC   -!- FUNCTION: Probable ATP-binding RNA helicase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX49/DBP8
CC       subfamily. {ECO:0000305}.
DR   EMBL; L13612; AAA16339.1; -; Unassigned_DNA.
DR   EMBL; Z23266; CAA80804.1; -; Genomic_DNA.
DR   EMBL; AE013599; AAF58994.1; -; Genomic_DNA.
DR   EMBL; AY058728; AAL13957.1; -; mRNA.
DR   PIR; S38329; S38329.
DR   RefSeq; NP_476927.1; NM_057579.5.
DR   SMR; Q07886; -.
DR   IntAct; Q07886; 3.
DR   STRING; 7227.FBpp0087645; -.
DR   PaxDb; Q07886; -.
DR   PRIDE; Q07886; -.
DR   EnsemblMetazoa; FBtr0088564; FBpp0087645; FBgn0010220.
DR   GeneID; 35917; -.
DR   KEGG; dme:Dmel_CG12759; -.
DR   CTD; 35917; -.
DR   FlyBase; FBgn0010220; Dbp45A.
DR   eggNOG; KOG0340; Eukaryota.
DR   eggNOG; ENOG410XPYC; LUCA.
DR   GeneTree; ENSGT00730000111231; -.
DR   InParanoid; Q07886; -.
DR   KO; K14778; -.
DR   OMA; TEHPIDQ; -.
DR   OrthoDB; 744428at2759; -.
DR   PhylomeDB; Q07886; -.
DR   Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR   GenomeRNAi; 35917; -.
DR   PRO; PR:Q07886; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0010220; Expressed in 32 organ(s), highest expression level in embryo.
DR   Genevisible; Q07886; DM.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase.
DR   GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; HMP:FlyBase.
DR   GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; RNA-binding.
FT   CHAIN         1    521       Probable ATP-dependent RNA helicase
FT                                Dbp45A.
FT                                /FTId=PRO_0000055004.
FT   DOMAIN       38    209       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      237    386       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND      51     58       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF         7     35       Q motif.
FT   MOTIF       157    160       DEAD box.
FT   CONFLICT    161    161       R -> P (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    211    217       SQDSDVA -> CEASAVR (in Ref. 1; AAA16339).
FT                                {ECO:0000305}.
FT   CONFLICT    224    225       QR -> HG (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    228    229       LC -> VS (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    234    234       R -> P (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    327    327       M -> I (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    407    407       D -> C (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    456    456       P -> S (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    493    493       D -> Y (in Ref. 1; AAA16339/CAA80804).
FT                                {ECO:0000305}.
FT   CONFLICT    517    521       KKDKA -> NKSKGKIKRDV (in Ref. 1;
FT                                AAA16339). {ECO:0000305}.
SQ   SEQUENCE   521 AA;  59678 MW;  89C68C43CD947913 CRC64;
     MQRKEANPFQ ILGLRPWLVK QLTKLGLKGA TPIQQKCIPA ILAGQDCIGA AKTGSGKTFA
     FALPILERLS EEPVSHFALV LTPTHELAYQ ISEQFLVAGQ AMGVRVCVVS GGTDQMVESQ
     KLMQRPHIVV AMPGRLADHL TGCDTFSFDN LKYLVVDEAD RMLNGDFDES LSIIERCLPK
     TRQNLFFSAT MKDFIKESSI FPIASDCFEW SQDSDVATVE TLDQRYLLCA DYDRDMVLIE
     ALRKYREENE NANVMIFTNT KKYCQLLSMT LKNMEIDNVC LHGFMRQKER VAALSRFKSN
     QIRTLIATDV AARGLDIPSV ELVMNHMLPR TPKEYIHRVG RTARAGRKGM SISIFRFPRD
     LELLAAIEEE INTKLTEHPI DQRMVERIFM QVNVTRRESE MQLDNNDFDE RAQNYRRKTW
     IMEGKDPDQM EALYRKKQKD KLREIRRKRK LQHAEPAASE EGKALLQDER FKSVDSARFE
     KKGKGRSRAT QEDTPTKPLK RLNKEKPVAQ KGRADVKKDK A
//
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