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Database: UniProt
Entry: Q09728
LinkDB: Q09728
Original site: Q09728 
ID   CUF1_SCHPO              Reviewed;         411 AA.
AC   Q09728;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   13-FEB-2019, entry version 137.
DE   RecName: Full=Metal-binding regulatory protein cuf1;
GN   Name=cuf1; ORFNames=SPAC31A2.11c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=FY254;
RX   PubMed=10593913; DOI=10.1074/jbc.274.51.36252;
RA   Labbe S., Pena M.M.O., Fernandes A.R., Thiele D.J.;
RT   "A copper-sensing transcription factor regulates iron uptake genes in
RT   Schizosaccharomyces pombe.";
RL   J. Biol. Chem. 274:36252-36260(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF LYS-13; ARG-16;
RP   ARG-19; LYS-24; ARG-28; LYS-45; ARG-47; ARG-50; ARG-53; CYS-328;
RP   CYS-330; CYS-334; CYS-336 AND CYS-339.
RX   PubMed=16467469; DOI=10.1128/EC.5.2.277-292.2006;
RA   Beaudoin J., Labbe S.;
RT   "Copper induces cytoplasmic retention of fission yeast transcription
RT   factor cuf1.";
RL   Eukaryot. Cell 5:277-292(2006).
CC   -!- FUNCTION: Copper-sensing transcription factor that regulates iron
CC       uptake genes. Under copper starvation conditions activates the
CC       transcription of the copper transport genes, ctr4, ctr5 and ctr6.
CC       {ECO:0000269|PubMed:10593913, ECO:0000269|PubMed:16467469}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16467469}.
CC       Nucleus {ECO:0000269|PubMed:16467469}. Note=Cytoplasmic in
CC       presence of excess copper ions. The Cys-His motif (328-342)
CC       interacts with the N-terminal nuclear localization signal region
CC       leading to sequestration in the cytoplasm. Nuclear under copper
CC       starvation conditions.
DR   EMBL; AF175404; AAD51063.1; -; Genomic_DNA.
DR   EMBL; AJ243832; CAB52304.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAA90469.1; -; Genomic_DNA.
DR   PIR; T38609; S58105.
DR   RefSeq; NP_592923.1; NM_001018324.2.
DR   ProteinModelPortal; Q09728; -.
DR   SMR; Q09728; -.
DR   BioGrid; 279627; 8.
DR   STRING; 4896.SPAC31A2.11c.1; -.
DR   MaxQB; Q09728; -.
DR   PaxDb; Q09728; -.
DR   PRIDE; Q09728; -.
DR   EnsemblFungi; SPAC31A2.11c.1; SPAC31A2.11c.1:pep; SPAC31A2.11c.
DR   GeneID; 2543198; -.
DR   KEGG; spo:SPAC31A2.11c; -.
DR   EuPathDB; FungiDB:SPAC31A2.11c; -.
DR   PomBase; SPAC31A2.11c; cuf1.
DR   InParanoid; Q09728; -.
DR   OMA; MACMKCI; -.
DR   PRO; PR:Q09728; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0005507; F:copper ion binding; IMP:PomBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:PomBase.
DR   GO; GO:0000978; F:RNA polymerase II proximal promoter sequence-specific DNA binding; IDA:PomBase.
DR   GO; GO:0006878; P:cellular copper ion homeostasis; IMP:PomBase.
DR   GO; GO:0006879; P:cellular iron ion homeostasis; IMP:PomBase.
DR   GO; GO:0035874; P:cellular response to copper ion starvation; IC:GOC-OWL.
DR   GO; GO:0055070; P:copper ion homeostasis; IMP:PomBase.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:PomBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:PomBase.
DR   Gene3D; 3.90.430.10; -; 1.
DR   InterPro; IPR001083; Cu_fist_DNA-bd_dom.
DR   InterPro; IPR036395; Cu_fist_DNA-bd_dom_sf.
DR   Pfam; PF00649; Copper-fist; 1.
DR   PRINTS; PR00617; COPPERFIST.
DR   SMART; SM01090; Copper-fist; 1.
DR   SMART; SM00412; Cu_FIST; 1.
DR   SUPFAM; SSF57879; SSF57879; 1.
DR   PROSITE; PS01119; COPPER_FIST_1; 1.
DR   PROSITE; PS50073; COPPER_FIST_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Copper; Cytoplasm; DNA-binding; Metal-binding;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation;
KW   Zinc.
FT   CHAIN         1    411       Metal-binding regulatory protein cuf1.
FT                                /FTId=PRO_0000194930.
FT   DNA_BIND      1     40       Copper-fist. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00055}.
FT   METAL        11     11       Zinc. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00055}.
FT   METAL        14     14       Zinc. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00055}.
FT   METAL        23     23       Zinc. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00055}.
FT   METAL        25     25       Zinc. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00055}.
FT   MUTAGEN      13     13       K->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      16     16       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      19     19       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      24     24       K->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      28     28       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      45     45       K->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      47     47       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      50     50       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN      53     53       R->A: No nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN     328    328       C->A: Constitutive nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN     330    330       C->A: Constitutive nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN     334    334       C->A: Constitutive nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN     336    336       C->A: Constitutive nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
FT   MUTAGEN     339    339       C->A: Constitutive nuclear localization.
FT                                {ECO:0000269|PubMed:16467469}.
SQ   SEQUENCE   411 AA;  45473 MW;  DB18E877B65D5699 CRC64;
     MVVINNVKMA CMKCIRGHRS STCKHNDREL FPIRPKGRPI SQCEKCRIAR ITRHLHVKCT
     CNSRKKGSKC STSSTTDLDS SSASNSSCSI PSSISEKLLP RDNVKTHCPK RSASCCGKKP
     DVMPLKINLE SQTDFMGMPL QSQRPHSESY RMLPEPEKFK SEYGYPSQFL PIEKLTSNVA
     YPPNYNNYLK SPYQQPTNFP PEIQYNYSHS PQHSIQEAEE AAVYGPPVYR SGYQILYNNN
     TDSIAAAAAT HDLYPQPDVP LTFAMLADGN YVPLPSSTNT YGPSNSYGYE ININESTNHV
     DSSYLPHPIQ LSNYFTLPSS CAQADAACQC GDNCECLGCL THPNNATTLA ALNHISALEK
     ETISHTDLHH TFKHEVNSSN NYELTNDELA ASSPLYTSSS VPPSHITTGS T
//
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