ID Q0A9Y1_ALKEH Unreviewed; 1252 AA.
AC Q0A9Y1;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 105.
DE RecName: Full=nitrate reductase (quinone) {ECO:0000256|ARBA:ARBA00012500};
DE EC=1.7.5.1 {ECO:0000256|ARBA:ARBA00012500};
GN OrderedLocusNames=Mlg_1003 {ECO:0000313|EMBL:ABI56356.1};
OS Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Alkalilimnicola.
OX NCBI_TaxID=187272 {ECO:0000313|EMBL:ABI56356.1, ECO:0000313|Proteomes:UP000001962};
RN [1] {ECO:0000313|Proteomes:UP000001962}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-1101 / DSM 17681 / MLHE-1
RC {ECO:0000313|Proteomes:UP000001962};
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Sims D., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Oremland R.S.,
RA Hoeft S.E., Switzer-Blum J., Kulp T., King G., Tabita R., Witte B.,
RA Santini J.M., Basu P., Hollibaugh J.T., Xie G., Stolz J.F., Richardson P.;
RT "Complete sequence of Alkalilimnicola ehrilichei MLHE-1.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinol + nitrate = a quinone + H2O + nitrite;
CC Xref=Rhea:RHEA:56144, ChEBI:CHEBI:15377, ChEBI:CHEBI:16301,
CC ChEBI:CHEBI:17632, ChEBI:CHEBI:24646, ChEBI:CHEBI:132124; EC=1.7.5.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001854};
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. {ECO:0000256|ARBA:ARBA00010312}.
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DR EMBL; CP000453; ABI56356.1; -; Genomic_DNA.
DR RefSeq; WP_011628751.1; NC_008340.1.
DR AlphaFoldDB; Q0A9Y1; -.
DR KEGG; aeh:Mlg_1003; -.
DR eggNOG; COG5013; Bacteria.
DR HOGENOM; CLU_000422_14_1_6; -.
DR OrthoDB; 9759518at2; -.
DR Proteomes; UP000001962; Chromosome.
DR GO; GO:0009325; C:nitrate reductase complex; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0008940; F:nitrate reductase activity; IEA:InterPro.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042126; P:nitrate metabolic process; IEA:InterPro.
DR CDD; cd02776; MopB_CT_Nitrate-R-NarG-like; 1.
DR CDD; cd02750; MopB_Nitrate-R-NarG-like; 1.
DR Gene3D; 3.40.50.12440; -; 1.
DR Gene3D; 4.10.1200.10; nitrate reductase tail; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR037943; MopB_CT_Nitrate-R-NarG-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR InterPro; IPR006468; NarG.
DR InterPro; IPR028189; Nitr_red_alph_N.
DR InterPro; IPR044906; Nitr_red_alph_N_sf.
DR NCBIfam; TIGR01580; narG; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR PANTHER; PTHR43105:SF2; RESPIRATORY NITRATE REDUCTASE 2 ALPHA CHAIN; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR Pfam; PF14710; Nitr_red_alph_N; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
DR PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000001962}.
FT DOMAIN 43..107
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 1252 AA; 141780 MW; 274CBE74DC2005BE CRC64;
MSYFLDRLTF FKRVKDGFSG GHGEVRDESR RWEDAYRSRW QHDKVVRSTH GVNCTGSCSW
KIYVKNGLVT WETQQTDYPR TRPELPNHEP RGCGRGASYS WYIYSANRLK YPMIRGTLLR
MWREARKKHK DPVDAWASIV GDPKKAELYK SRRGLGDMVR SDWDEVNELV ASANIHTIKE
HGPDRVIGFS PIPAMSMVSY AAGSRYMSLL GGVCMSFYDW YCDLPPSSPQ TWGEQTDVPE
SADWYNAGFI MMWGSNVPQT RTPDAHFMTE VRYKGTEIVT VCPDYSEASK FGDIWLNPQQ
GTDSALGMAL GHVILKEFHV DNPSDYFRDY ARQYTDMPCL VRLEKTDKGY QADRFLRASD
LDKALGQKNN PEWKTIAWDE KSDSLVVPNG SIGFRWGEDG QWNLEEKEAS GKETKLQLSH
LDSRDEVALV GFPYFGGQEH ELGRFTANPQ EEVIYRKVPV RKIKLADGEE VLAASVYDLI
TAHYGVDRGL DCENTPKSYD ENKPYTPAWQ EQITGVPRDK VIKVARRFAD NANKTRGKSM
IIIGAAMNHW YHMDMNYRSV INMLVFCGCI GQSGGGWAHY VGQEKLRPQT GWQPLAFALD
WARPPRHMNS TSFFYAHTDQ WRYEKLNVAD MLSPLANKED FQGSLIDFNV RSERMGWLPS
APQLGANPLE VAKEAAKAGK DPKDYVVEQL KSGRLRMACE DPDNPANFPR NLFVWRSNLL
GSSGKGHEYF LKHLLGTKHG VQGKDLGEEG AEKPQEVVWR EEETRGKLDL LTTLDFRMST
TCLYSDVVLP TATWYEKDDL NTSDMHPFIH PLCEAVNPCW ESRTDWEIYK GLAKTFSKLV
EGHLGKETDI VTLPLLHDSP AELGQALDVK EWHKGECDPI PGKTMPALVP VERDYPNVYA
RFTALGPLMT KLGNGGKGIS WNTEHEVDLL GRLNGQHRKE GAANQGLPRI NTAIEAADTI
LSLAPETNGE VAVKAWAALS KQTGRDHTHL ALPREEEKLR FRDLAAQPRK IISSPTWSGL
ESEHVSYNAG YTNVHELIPW RTLSGRQQLY QDHPWMRAFG EGFVCYRPPV NMRTTQTVQG
VRGNGNEEIA LNWITPHQKW GIHSTYTDNL IMLTLSRGGP CVWISEIDAK KVGIVDNDWI
ECFNSNGSLV ARAVVSQRVR EGMVMMYHAQ EKLVNTPGSE ITGERGGIHN SVTRAVIKPT
HMIGGYAQLS YGFNYYGTVG SNRDEFVVVR KMDRVDWLDG DDESIYDAEE QA
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