ID Q0APE2_MARMM Unreviewed; 774 AA.
AC Q0APE2;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 89.
DE SubName: Full=Penicillin amidase {ECO:0000313|EMBL:ABI65845.1};
DE EC=3.5.1.11 {ECO:0000313|EMBL:ABI65845.1};
DE Flags: Precursor;
GN OrderedLocusNames=Mmar10_1553 {ECO:0000313|EMBL:ABI65845.1};
OS Maricaulis maris (strain MCS10).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Maricaulales; Maricaulaceae;
OC Maricaulis.
OX NCBI_TaxID=394221 {ECO:0000313|EMBL:ABI65845.1, ECO:0000313|Proteomes:UP000001964};
RN [1] {ECO:0000313|EMBL:ABI65845.1, ECO:0000313|Proteomes:UP000001964}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MCS10 {ECO:0000313|EMBL:ABI65845.1,
RC ECO:0000313|Proteomes:UP000001964};
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Viollier P.,
RA Stephens C., Richardson P.;
RT "Complete sequence of Maricaulis maris MCS10.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
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DR EMBL; CP000449; ABI65845.1; -; Genomic_DNA.
DR RefSeq; WP_011643492.1; NC_008347.1.
DR AlphaFoldDB; Q0APE2; -.
DR STRING; 394221.Mmar10_1553; -.
DR MEROPS; S45.003; -.
DR KEGG; mmr:Mmar10_1553; -.
DR eggNOG; COG2366; Bacteria.
DR HOGENOM; CLU_011790_0_1_5; -.
DR OrthoDB; 9760084at2; -.
DR Proteomes; UP000001964; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008953; F:penicillin amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABI65845.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000001964};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT ACT_SITE 254
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 192
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 326
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 329
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 774 AA; 84416 MW; 7C7A9DF780C1CFC6 CRC64;
MIKRVLRYLG WAVGGLVALV LVGALIVANR LYASLPQTTG EMTVAGLQAE ATIARDSHGV
PHIFAQSEHD GFYAMGVAHA QDRLWQMEIT RRALRGRLAE ILGEPGLDTD IFFRTMGLGT
ASDTAVDNLP DDVRAALQAY ADGVNAVISA PGFVPPPEFQ ILMFDPEPWQ PADTVVVYKA
IALDLFGNAF QEPRMAALVS QLGEQRTAEF IGRYPDDAPR SLTMADIGIE ATTLPPAEEL
APVMGPEDTG RDGSNNWVLS GSRTTTGLPI LANDPHLGLA APAIWYLARL TTPQGSVVGA
TLPGTPFVTL GRTDEIAWGF TNTGPDVGDL HAVTEADVTG QREEHITVRF GEDVIITRRE
TATGPVLDPK HFNYPVPDGS DFFALQWMLD EPDDATTGVG LHIIEADDWD GFVSAIRGFV
APQQSMVFAD SDGDIGFYAP ARIPVRDETG AWVSTIPFEE LPHTLNPDRG FVATANNKIV
PDDYPHYLTS EWYGVSRIRR IHDGISAAPV HDIETMQALQ VDTVSDTALR LLPVLLQAEP
ETQAGRDALA VLADWDANMA VDRPEPLIYS AWMRQLSADI YGDELGELLP AWHGDRRVFM
MDVLTGDLGH WCDNEAIART VTCQELTGPA LDQAMAETMA AYGADFTGWR WGDVHYARHA
HRPFSDFPVL SDWFSITTPV PGDGSTVNVA HYSYRTPGYE VFHGASYRAL YDMAAPDTSR
FMITTGQSGN VMSRHYDDLA PMWGRGEYIA IPTGWTMDAA PDGLSVLRLQ PGSQ
//
