ID Q0EZ74_9PROT Unreviewed; 1045 AA.
AC Q0EZ74;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Translation initiation factor IF-2 {ECO:0000256|ARBA:ARBA00020675, ECO:0000256|HAMAP-Rule:MF_00100};
GN Name=infB {ECO:0000256|HAMAP-Rule:MF_00100};
GN ORFNames=SPV1_07641 {ECO:0000313|EMBL:EAU54550.1};
OS Mariprofundus ferrooxydans PV-1.
OC Bacteria; Pseudomonadota; Zetaproteobacteria; Mariprofundales;
OC Mariprofundaceae; Mariprofundus.
OX NCBI_TaxID=314345 {ECO:0000313|EMBL:EAU54550.1, ECO:0000313|Proteomes:UP000005297};
RN [1] {ECO:0000313|EMBL:EAU54550.1, ECO:0000313|Proteomes:UP000005297}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PV-1 {ECO:0000313|EMBL:EAU54550.1,
RC ECO:0000313|Proteomes:UP000005297};
RA Emerson D., Ferriera S., Johnson J., Kravitz S., Halpern A., Remington K.,
RA Beeson K., Tran B., Rogers Y.-H., Friedman R., Venter J.C.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000256|HAMAP-Rule:MF_00100,
CC ECO:0000256|RuleBase:RU000644}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00100}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733, ECO:0000256|HAMAP-Rule:MF_00100,
CC ECO:0000256|RuleBase:RU000644}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00100}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAU54550.1}.
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DR EMBL; AATS01000007; EAU54550.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0EZ74; -.
DR STRING; 314344.AL013_00355; -.
DR eggNOG; COG0532; Bacteria.
DR HOGENOM; CLU_006301_5_1_0; -.
DR InParanoid; Q0EZ74; -.
DR OrthoDB; 5287317at2; -.
DR Proteomes; UP000005297; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule.
DR CDD; cd01887; IF2_eIF5B; 1.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR CDD; cd03692; mtIF2_IVc; 1.
DR Gene3D; 1.10.10.2480; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR006847; IF2_N.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00487; IF-2; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF5; TR-TYPE G DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR Pfam; PF04760; IF2_N; 1.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 2.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00100};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP-
KW Rule:MF_00100};
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540, ECO:0000256|HAMAP-
KW Rule:MF_00100};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00100};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00100}; Reference proteome {ECO:0000313|Proteomes:UP000005297}.
FT DOMAIN 545..713
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT REGION 55..450
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 256..270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 554..561
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
FT BINDING 601..605
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
FT BINDING 655..658
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00100"
SQ SEQUENCE 1045 AA; 110843 MW; 8A89189F6D0A6090 CRC64;
MAKRILDLAK ELGVDAAEIQ RVAVQCNVVV SGPTSTLDTD DLKKIRASLQ SKVEAAPAKT
GGKTLTLNRP MVAGQPRSGG RVEGRGRSVE VSVRRRHTPV KAQPTVAEQT PQADAVETPA
AAAPKAKPLA PAQRAAAAVE QRKLTEAANK AAAEQQKAAD AKAAASEPAA AEAPKVPASK
PEVAAQPAVS RETATKSPVT RQPIKRQAGV ASKPTDTPGV VRRSAVNPAE VARSKIAAAA
AARTAAQEKS NQERVARQRA AQTRNTPAGT GGAAAQGRPG QVGAPARTRQ APGGPNRSPQ
DRRPAQGAQS PGHKGPRTTI RRGLTPAQIA ASKAPSSSLK QIEQKISEER ATRRKTQQRP
ASGARPGGAA NQGMTRRTPS VAISPSADAP AAPTGPQRRR GAPGAQRGQR RLSPAEKAAR
RDYASKRNVV LTPQQEERMA RLARGSKRRN QITKDDENFV VRTVEVTDPI MIQELASRMA
IKSAEVVKKL FEMGSMVTVN EGIDGETAVL IVEEFGHKAK IINAGAVEDV LIEDVSEDDD
TDLQPRPPVV VVMGHVDHGK TSILDALRKA HVAAGEAGGI TQHIGAYMVK LASGERVVFI
DTPGHEAFTS LRARGAGMTD VAVLVVAADD GVMPQTIEAL NHAKAAGVPM IVAVNKMDKE
GADPEKVMRQ LADHGVLSED WGGDTIFVPV SAHTGLGLDE LLDMLALQTE ILELNANPKR
RGKGIVIESR LDRGRGPVAT VLVQNGTFKQ GDTVVVGSVT GRIRAIVDEN AKQHRTAGPS
IPFELLGLDS VPESGQEIFT VESEKQARDI VRYRKDKERE QGAEAMKRAS MDELFANLQS
GMKEVPVVIK GDVTGSVDAM ADSLAKAGTD EVKVKVVHKG IGGITESDVM LASASNAIVI
GFNVRPEAKA KRLAEQEGID VRFYKIIYDA IDDMKAAMAG VLSPDKVEKV TGSADVREVF
QAPKIGAIAG CYVTDGYVTR GSNVRVLRDG VVVYDGVLAS LRRFRDDVKE VKTGFECGIG
VEKFNDIKEG DSFEFYIIEE VAATL
//
