ID Q0FGQ8_9RHOB Unreviewed; 1151 AA.
AC Q0FGQ8;
DT 17-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 17-OCT-2006, sequence version 1.
DT 24-JAN-2024, entry version 87.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=OM2255_03615 {ECO:0000313|EMBL:EAU52581.1};
OS Rhodobacterales bacterium HTCC2255.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales.
OX NCBI_TaxID=367336 {ECO:0000313|EMBL:EAU52581.1, ECO:0000313|Proteomes:UP000009381};
RN [1] {ECO:0000313|EMBL:EAU52581.1, ECO:0000313|Proteomes:UP000009381}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTCC2255 {ECO:0000313|EMBL:EAU52581.1,
RC ECO:0000313|Proteomes:UP000009381};
RA Giovannoni S., Cho J.-C., Ferriera S., Johnson J., Kravitz S., Halpern A.,
RA Remington K., Beeson K., Tran B., Rogers Y.-H., Friedman R., Venter J.C.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EAU52581.1}.
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DR EMBL; AATR01000001; EAU52581.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0FGQ8; -.
DR eggNOG; COG1197; Bacteria.
DR HOGENOM; CLU_005122_1_3_5; -.
DR OrthoDB; 9804325at2; -.
DR BioCyc; RBAC367336:G1GNW-168-MONOMER; -.
DR Proteomes; UP000009381; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000009381}.
FT DOMAIN 615..776
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 797..951
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1151 AA; 128892 MW; 9C0FA90B13FD6296 CRC64;
MNSFSKNIIN LSGAPDGFDA NILSNFITEK QKSIIFVARD DKRLDLMRKS LWFFSPNIPI
LDFPSWDCLP YDRVSPNADV SSARMATLAA LSSGFEAPIV LLTTLNAITQ YIPNRTIVSN
NSFVAIVGRT INVKELRAYF SKMGFVQTPT VTEPGDYAIR GGIIDVFPPG ESGPVRMDLF
GDELESARRF DPVTQRTVEN LDRIEFAPVS EVILDDVSIN RFRNNYRKEF GSAGLDDPLY
EAISAGRKHQ GYEHWAPYFH DGMETIFDHL PNAVIFMDEN IERIHTSRWD GINDQYEARL
EALNSKNRLE TVYKPIKPEL FYVSPDDLFD LLNNREQRKF IVLPQPTGPN SLDMRARIGR
NFAPERQNEE LGLFEEFAKH IIEKRKSTSV IIASMSLGAR ERLYGLLQDQ GLSGMVNIKT
WKDINQSIGS ISLAVWHLEH GFEAPGLTII SEQDVLGELI IRKTNKKRRA KDFLTEASSL
SVGDLVVHVD HGVGRYRGLE TVKAAGAPHD CLLLEYANND RLYLPVENVE LLSRYGHETG
LLDRLGGSAW QAKKAKLKER IREMADKLLR IAAERSLRKS EILEVSPDKW NAFCARFPYV
ETDDQLNAIE DVVSDMSSGK PMDRLICGDV GFGKTEVALR AAFIAASAGT QAAIIAPTTL
LARQHFKSFE ERFRGTGIRV KQLSRFVTTS QMKKNREALR DGAVEIIIGT HALLAKDIKF
ANLGLLIIDE EQKFGVGHKE RLKQLRSDIH VLTLTATPIP RTLQLSLSGV RELSVIGTPP
IDRLAIRTYV SEFDTVTLRE ALLREHYRGG QSFFVVPRIS DLPEIEAFIE EQVPEINHVV
AHGQMPAGEL DERMNAFYDG KFGILVATTI VESGLDIPTA NTIIIHRADM FGLSQLYQIR
GRVGRSKTRA YAYLTTKPRQ KLTHAAIKRL RVLGSLDSLG AGFMLASQDL DIRGAGNILG
EEQSGNVREV GYELYQEMLE DAIAKIKSGQ MEGLTDEGSW SPQINLGVPV LIPETYVQDL
DVRLGLYRRL SALAKKIELE AFAAELIDRF GKLPTEVDML LRVVRIKGMC RAAGIAKFDS
GPKGAVIQFY QDKFISPSGL AQYLLDSRGT AKIKDNKLIV LRDWTNDDKK VKGAYIIARE
LAVMAKSQKT N
//
