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Database: UniProt
Entry: Q0JA75
LinkDB: Q0JA75
Original site: Q0JA75 
ID   CADH7_ORYSJ             Reviewed;         379 AA.
AC   Q0JA75; A0A0N7KJP2; Q7F9B4;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   10-APR-2019, entry version 97.
DE   RecName: Full=Cinnamyl alcohol dehydrogenase 7 {ECO:0000305};
DE            Short=OsCAD7 {ECO:0000303|PubMed:15452707};
DE            EC=1.1.1.195 {ECO:0000269|PubMed:19116760};
DE   AltName: Full=Protein FLEXIBLE CULM 1 {ECO:0000303|PubMed:19116760};
GN   Name=CAD7 {ECO:0000303|PubMed:15452707};
GN   Synonyms=FC1 {ECO:0000303|PubMed:19116760};
GN   OrderedLocusNames=Os04g0612700, LOC_Os04g52280;
GN   ORFNames=OSJNBa0070C17.13;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J.,
RA   Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y.,
RA   Weng Q., Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D.,
RA   Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J.,
RA   Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H.,
RA   Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y.,
RA   Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W.,
RA   Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W.,
RA   Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y.,
RA   Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15452707; DOI=10.1007/s00425-004-1385-4;
RA   Tobias C.M., Chow E.K.;
RT   "Structure of the cinnamyl-alcohol dehydrogenase gene family in rice
RT   and promoter activity of a member associated with lignification.";
RL   Planta 220:678-688(2005).
RN   [7]
RP   FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=19116760; DOI=10.1007/s11103-008-9448-8;
RA   Li X., Yang Y., Yao J., Chen G., Li X., Zhang Q., Wu C.;
RT   "FLEXIBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controls
RT   culm mechanical strength in rice.";
RL   Plant Mol. Biol. 69:685-697(2009).
CC   -!- FUNCTION: Involved in lignin biosynthesis. May catalyze the final
CC       step specific for the production of lignin monomers, like
CC       coniferyl alcohol, sinapyl alcohol and 4-coumaryl alcohol.
CC       {ECO:0000269|PubMed:19116760}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-cinnamyl alcohol + NADP(+) = (E)-cinnamaldehyde +
CC         H(+) + NADPH; Xref=Rhea:RHEA:10392, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16731, ChEBI:CHEBI:33227, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.1.1.195;
CC         Evidence={ECO:0000269|PubMed:19116760};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:O49482};
CC       Note=Binds 2 Zn(2+) ions per subunit.
CC       {ECO:0000250|UniProtKB:O49482};
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid
CC       biosynthesis.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:O49482}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, first internodes and
CC       panicles. Expressed in the vascular bundles and sclerenchyma cells
CC       below the epidermis in leaves and stems.
CC       {ECO:0000269|PubMed:19116760}.
CC   -!- DISRUPTION PHENOTYPE: Reduced lignin content, late heading time,
CC       semi-dwarf and flexible culm. {ECO:0000269|PubMed:19116760}.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAE05206.3; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AL731610; CAE05206.3; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008210; BAF15762.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS90969.1; -; Genomic_DNA.
DR   EMBL; AK102452; BAG95561.1; -; mRNA.
DR   RefSeq; XP_015635610.1; XM_015780124.1.
DR   UniGene; Os.32904; -.
DR   ProteinModelPortal; Q0JA75; -.
DR   SMR; Q0JA75; -.
DR   STRING; 4530.OS04T0612700-01; -.
DR   PaxDb; Q0JA75; -.
DR   EnsemblPlants; Os04t0612700-01; Os04t0612700-01; Os04g0612700.
DR   GeneID; 4336968; -.
DR   Gramene; Os04t0612700-01; Os04t0612700-01; Os04g0612700.
DR   KEGG; osa:4336968; -.
DR   eggNOG; KOG0023; Eukaryota.
DR   eggNOG; COG1064; LUCA.
DR   HOGENOM; HOG000294667; -.
DR   InParanoid; Q0JA75; -.
DR   KO; K00083; -.
DR   OMA; YSDIHTL; -.
DR   OrthoDB; 625659at2759; -.
DR   BRENDA; 1.1.1.195; 8948.
DR   UniPathway; UPA00711; -.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q0JA75; OS.
DR   GO; GO:0045551; F:cinnamyl-alcohol dehydrogenase activity; IMP:UniProtKB.
DR   GO; GO:0052747; F:sinapyl alcohol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009809; P:lignin biosynthetic process; IMP:UniProtKB.
DR   InterPro; IPR013149; ADH_C.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Lignin biosynthesis; Metal-binding; NADP;
KW   Oxidoreductase; Reference proteome; Zinc.
FT   CHAIN         1    379       Cinnamyl alcohol dehydrogenase 7.
FT                                /FTId=PRO_0000382646.
FT   NP_BIND     210    215       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   NP_BIND     233    238       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   NP_BIND     320    322       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   METAL        60     60       Zinc 1; catalytic.
FT                                {ECO:0000250|UniProtKB:O49482}.
FT   METAL        82     82       Zinc 1; catalytic.
FT                                {ECO:0000250|UniProtKB:O49482}.
FT   METAL        83     83       Zinc 1; catalytic.
FT                                {ECO:0000250|UniProtKB:O49482}.
FT   METAL       113    113       Zinc 2. {ECO:0000250|UniProtKB:O49482}.
FT   METAL       116    116       Zinc 2. {ECO:0000250|UniProtKB:O49482}.
FT   METAL       119    119       Zinc 2. {ECO:0000250|UniProtKB:O49482}.
FT   METAL       127    127       Zinc 2. {ECO:0000250|UniProtKB:O49482}.
FT   METAL       185    185       Zinc 1; catalytic.
FT                                {ECO:0000250|UniProtKB:O49482}.
FT   BINDING      62     62       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   BINDING     189    189       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   BINDING     273    273       NADP. {ECO:0000250|UniProtKB:O49482}.
FT   BINDING     297    297       NADP; via carbonyl oxygen.
FT                                {ECO:0000250|UniProtKB:O49482}.
SQ   SEQUENCE   379 AA;  39566 MW;  28A2B28B13FA7866 CRC64;
     MAPTTTATAA AEQAPPPQHT RKAVGLAAHD DSGHLTPIRI SRRKTGDDDV AIKVLYCGIC
     HSDLHTIKNE WRNAVYPVVA GHEITGVVTE VGKNVARFKA GDEVGVGCMV NTCGGCESCR
     DGCENYCSGG VVFTYNSVDR DGTRTYGGYS DAVVVSQRFV VRFPSSAGGG AGAALPLDSG
     APLLCAGVTV YAPMRQHGLC EAGKHVGVVG LGGLGHVAVK FARAFGMRVT VISTSPVKRQ
     EALERLGADG FIVSTNASEM KAAMGTMHGI INTASASTSM HSYLALLKPK GKMILVGLPE
     KPLQIPTFAL VGGGKILAGS CMGSISETQE MIDFAAEHGV AADIELIGAD EVNTAMERLA
     KGDVRYRFVV DIGNTLRSD
//
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