ID   Q0K0C1_CUPNH            Unreviewed;       395 AA.
AC   Q0K0C1;
DT   03-OCT-2006, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2006, sequence version 1.
DT   27-MAR-2024, entry version 113.
DE   SubName: Full=Acetyl-CoA acetyltransferase {ECO:0000313|EMBL:CAJ96553.1};
DE            EC=2.3.1.9 {ECO:0000313|EMBL:CAJ96553.1};
GN   OrderedLocusNames=H16_A1713 {ECO:0000313|EMBL:CAJ92837.1}, H16_B1771
GN   {ECO:0000313|EMBL:CAJ96553.1};
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666 {ECO:0000313|EMBL:CAJ96553.1, ECO:0000313|Proteomes:UP000008210};
RN   [1] {ECO:0000313|EMBL:CAJ96553.1, ECO:0000313|Proteomes:UP000008210}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier
RC   337 {ECO:0000313|Proteomes:UP000008210}, and H16
RC   {ECO:0000313|EMBL:CAJ96553.1};
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Potter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
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DR   EMBL; AM260479; CAJ92837.1; -; Genomic_DNA.
DR   EMBL; AM260480; CAJ96553.1; -; Genomic_DNA.
DR   RefSeq; WP_011615250.1; NZ_CP039288.1.
DR   AlphaFoldDB; Q0K0C1; -.
DR   STRING; 381666.H16_A1713; -.
DR   GeneID; 57647672; -.
DR   KEGG; reh:H16_A1713; -.
DR   KEGG; reh:H16_B1771; -.
DR   eggNOG; COG0183; Bacteria.
DR   HOGENOM; CLU_031026_0_0_4; -.
DR   OrthoDB; 8558405at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   Proteomes; UP000008210; Chromosome 2.
DR   GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020610; Thiolase_AS.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR   PANTHER; PTHR18919; ACETYL-COA C-ACYLTRANSFERASE; 1.
DR   PANTHER; PTHR18919:SF153; TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL; 1.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00737; THIOLASE_2; 1.
DR   PROSITE; PS00099; THIOLASE_3; 1.
PE   3: Inferred from homology;
KW   Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW   ECO:0000313|EMBL:CAJ96553.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008210};
KW   Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:CAJ96553.1}.
FT   DOMAIN          6..263
FT                   /note="Thiolase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00108"
FT   DOMAIN          272..392
FT                   /note="Thiolase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF02803"
FT   ACT_SITE        90
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT   ACT_SITE        350
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT   ACT_SITE        380
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ   SEQUENCE   395 AA;  40828 MW;  C266253702726C9D CRC64;
     MHARDVVLCQ PVRTAIGAYN GSLKAVPAVD LGAAAIRATV ARAGIDPGEV GSVVFGNVIQ
     AGNRMNPARQ AAIGAGLPVS VPAMTVNRVC GSGAQAIATA ADEVRLGYVD IAIAGGMENM
     DRAPYLMPSG RWGQRMGDSV LYDSMLHDGL FDAFSGEHSG WHTEDLVARY ALTREAQDRW
     AERSQRNFAL AQANGRFTGQ IAGVATKGKG GTAMFEVDEA NRPDTSLDTL GRLKPAFRPD
     GTITAGNAPG LNSGAAAMII AERALAEARG LQPLGRLAAY GVGAVEPGMF GLGPVPAVRQ
     ALQRAGWELG DIERIEINEA FAAVPLAVAV ELGLPHDIIN VDGGAIAHGH PIGATGAVLT
     TRLLHAMRAD GIRRGIVTLC IGGGQGIALA LETLA
//