GenomeNet

Database: UniProt
Entry: Q0UL22
LinkDB: Q0UL22
Original site: Q0UL22 
ID   DCL2_PHANO              Reviewed;        1399 AA.
AC   Q0UL22;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   31-JUL-2019, entry version 74.
DE   RecName: Full=Dicer-like protein 2;
DE   Includes:
DE     RecName: Full=Endoribonuclease DCL2;
DE              EC=3.1.26.-;
DE   Includes:
DE     RecName: Full=ATP-dependent helicase DCL2;
DE              EC=3.6.4.-;
GN   Name=DCL2; ORFNames=SNOG_07542;
OS   Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)
OS   (Glume blotch fungus) (Parastagonospora nodorum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
OC   Phaeosphaeriaceae; Parastagonospora.
OX   NCBI_TaxID=321614;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SN15 / ATCC MYA-4574 / FGSC 10173;
RX   PubMed=18024570; DOI=10.1105/tpc.107.052829;
RA   Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L.,
RA   Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E.,
RA   Torriani S.F.F., McDonald B.A., Oliver R.P.;
RT   "Dothideomycete-plant interactions illuminated by genome sequencing
RT   and EST analysis of the wheat pathogen Stagonospora nodorum.";
RL   Plant Cell 19:3347-3368(2007).
CC   -!- FUNCTION: Dicer-like endonuclease involved in cleaving double-
CC       stranded RNA in the RNA interference (RNAi) pathway. Produces 21
CC       to 25 bp dsRNAs (siRNAs) which target the selective destruction of
CC       homologous RNAs leading to sequence-specific suppression of gene
CC       expression, called post-transcriptional gene silencing (PTGS).
CC       Part of a broad host defense response against viral infection and
CC       transposons (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00657}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAT85008.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; CH445335; EAT85008.2; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001797877.1; XM_001797825.1.
DR   STRING; 13684.SNOT_07542; -.
DR   PRIDE; Q0UL22; -.
DR   GeneID; 5974770; -.
DR   KEGG; pno:SNOG_07542; -.
DR   InParanoid; Q0UL22; -.
DR   OrthoDB; 1337630at2759; -.
DR   Proteomes; UP000001055; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0016442; C:RISC complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0030422; P:production of siRNA involved in RNA interference; IBA:GO_Central.
DR   GO; GO:0050688; P:regulation of defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd00593; RIBOc; 2.
DR   Gene3D; 1.10.1520.10; -; 2.
DR   Gene3D; 3.30.160.380; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR038248; Dicer_dimer_sf.
DR   InterPro; IPR005034; Dicer_dimerisation_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF03368; Dicer_dimer; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00636; Ribonuclease_3; 2.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00535; RIBOc; 2.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF69065; SSF69065; 2.
DR   PROSITE; PS51327; DICER_DSRBF; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS00517; RNASE_3_1; 1.
DR   PROSITE; PS50142; RNASE_3_2; 2.
PE   3: Inferred from homology;
KW   Antiviral defense; Antiviral protein; ATP-binding; Complete proteome;
KW   Helicase; Hydrolase; Magnesium; Manganese; Metal-binding;
KW   Nucleotide-binding; Reference proteome; Repeat; RNA-binding.
FT   CHAIN         1   1399       Dicer-like protein 2.
FT                                /FTId=PRO_0000306797.
FT   DOMAIN       18    194       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      364    549       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN      562    656       Dicer dsRNA-binding fold.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00657}.
FT   DOMAIN      897   1054       RNase III 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1094   1270       RNase III 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1301   1370       DRBM.
FT   NP_BIND      31     38       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       139    142       DEAH box.
FT   METAL      1133   1133       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1256   1256       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1259   1259       Magnesium or manganese. {ECO:0000250}.
FT   SITE       1252   1252       Important for activity. {ECO:0000250}.
SQ   SEQUENCE   1399 AA;  157596 MW;  375777F3770D6455 CRC64;
     MADSHDAPFR LRSYQAEMVE ESMQSNIICV MDTGSGKTHI AIDRTRAELE ICRPDKIVWF
     LAPTVTLCEQ QFAVFKSNLP GYGIQLLSGK DNLDHWTDQG VWDDVLLNIR IVLSTHQVLL
     DALSHGFVKM RNLSLLIFDE AHHCSLKHPA HRIMSDFYKP RIGTELPRIL GLSASPIKTA
     KVTSEDLQQI ERNLCATVKT PKLHRSELLR YVHRPHLMRI DYPVEPQDLQ SNMLSFLKSA
     YTNYDLQKDP WVSELLQQRQ QGHDVTKNIH KVFIGGKTYC RDQLRSLALK AEAMSQELGM
     SVMDWYLRGC ITQFSKMVHM SDSQLLDWSA DEKRHLLEIL RTLPSIDLNS HDIPPMSLGS
     MSHKLQLLIK FLVAEAKHDP EFTCLVFVEQ RVWVACIAEV LAIHPETRDL LRVGTFVGES
     ENSKRKANIA SISEPRNQQA TLENFRAGKL NLILATSVLE EGIDVSSCHL VVCFESPKNL
     KSFVQRRGRA RKEESKYVIF VPQAGRRRDP ESWQSLEEGM KAAYLDDLRQ VKLATEKEQQ
     SETGHRNFEV KSTGALLTLD NASQHLHHFC SILGAGPYID NRPQFEFTEI RPGVITARVI
     LPLTVDPEVR TACALDTWAT EKMAKQDAAF EAYKALYVAG LVNDNLLPAR QEADDELSEL
     QIPDHRPSLV PVSPTLDPWP LFARHQQQNP HVYYRTRLTL HTVDDKPKHL ILLTPKILPD
     IPELLLYWNS STKLKIESSW LHDVVLNDEE ISELKSVTYK ILYSVFHNRM ELNRRDFVWL
     VAPCDESGLL DSRIWLSEWR QHTCLATELI AQNSDWSLWG LVNQKGDARK YTPRSTSMNN
     QLWLLQLMQL PKRRDFLHPV LESANINDAY TKTDEMAAKD CIVDPVPAPY SVFALLLPSI
     LHRFGMAIIA ETLRTTLLGP VALDSAHSLL LTRALKSSAA DGNDNYQRLE FLGDCILKFI
     ATVHLMAANP KWPESHLTAK KGRIVSNGFL ARATIAAGLD RFMITKSFTG AKWAPRYAGD
     LLAETGPAVK EERSSKLIAD IIESLIGACY TVGGFEKAVL CVQTLLPLEP WISVPAANSI
     LHEAAPAEAD LMGLDVLETL IGYTFKKKPL LLEALTHASF SGPHVHCSYE RLEFLGDAVL
     DYIISKRLHA HSPELSHQKM HAIRTATVNA SFLAFRLFET TIDEETINKT SMRPESQKRA
     IWQFLRSGSP SLNANRDNAL RQHEQVRDEI IIGLNEAARF PWHLFALTDP PKFLSDMVES
     VIGAVYIDSL GDILTCEAIV RRLGILDCLD HILCNGVDCL HPKERLGHLA VDKGVQYARV
     GMNTEPNEGD KMYKCQVKVG GEDVGDVAEG LKRLNAETVA AWKAVGVLES RKDSAIEIVS
     DVEEFFDADD GGGISLDDP
//
DBGET integrated database retrieval system