ID Q0W5V6_METAR Unreviewed; 574 AA.
AC Q0W5V6;
DT 31-OCT-2006, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2006, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE SubName: Full=F420-nonreducing hydrogenase (Membrane-bound),large subunit {ECO:0000313|EMBL:CAJ36237.1};
DE EC=1.12.2.- {ECO:0000313|EMBL:CAJ36237.1};
GN Name=vhtA {ECO:0000313|EMBL:CAJ36237.1};
GN ORFNames=RCIX882 {ECO:0000313|EMBL:CAJ36237.1};
OS Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanocellales; Methanocellaceae; Methanocella.
OX NCBI_TaxID=351160 {ECO:0000313|EMBL:CAJ36237.1, ECO:0000313|Proteomes:UP000000663};
RN [1] {ECO:0000313|EMBL:CAJ36237.1, ECO:0000313|Proteomes:UP000000663}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22066 / NBRC 105507 / MRE50
RC {ECO:0000313|Proteomes:UP000000663};
RX PubMed=16857943; DOI=10.1126/science.1127062;
RA Erkel C., Kube M., Reinhardt R., Liesack W.;
RT "Genome of rice cluster I archaea -- the key methane producers in the rice
RT rhizosphere.";
RL Science 313:370-372(2006).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|ARBA:ARBA00001967,
CC ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC family. {ECO:0000256|ARBA:ARBA00009292, ECO:0000256|RuleBase:RU003896}.
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DR EMBL; AM114193; CAJ36237.1; -; Genomic_DNA.
DR AlphaFoldDB; Q0W5V6; -.
DR STRING; 351160.RCIX882; -.
DR KEGG; rci:RCIX882; -.
DR PATRIC; fig|351160.9.peg.2006; -.
DR eggNOG; arCOG01550; Archaea.
DR Proteomes; UP000000663; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958:SF4; HYDROGENASE EXPRESSION_FORMATION PROTEIN HUPK; 1.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003896};
KW Reference proteome {ECO:0000313|Proteomes:UP000000663}.
FT BINDING 22
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 41
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 44
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 44
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 508
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 563
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 566
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 569
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 574 AA; 62669 MW; 7CFB966CDF083B6A CRC64;
MEMKDGSVVD ARCSGTTYRG FEQMMIGRDP MDAVYFTQRV CGMCSTPHAT AAAGAVESLA
GATGQIPKDA LLVRNILNGL AWLRSHVENL YLSFLPDLAD PIYGDLLRYS DVGTALTGEL
AGRFNVPGYA STGHTAPGSA YTDAIRFVKL TGEAEAILGG RSPHSPVIVP GGVTVRPSAS
DLMKLKGCYA SIVDFLERRL TYPLSLDEWL ENTHSTQANP DFILNRIKSL PTGDLSMEKG
WNDMQLFSVF CSRMMSYDFL SMPIYVELDT LGGYPLYDQL IGFLNYGGFY RVRDRAGNFT
DGYSPVDGEE GTFEIPSGFT PGSMPNLFDS PGKADPAMVA EQVSGSFFSY SGGETALKPE
AGETSPSPRA ADIDYSGHKY SFVKAPRYGN VPCETGPLAR MINSREPYIM NIMQMMHFSN
PVFSSGKSYN RTSVYTRVLS RMQECLITAQ LLGRWIDDLE VHSDPRKYCV PVTPGRGEAG
ACMIEAPRGA LGHWMRLDED GRIAGYQIIS PTTWNASPRD AETKFGPIEL SLIGAKTTPR
GNIPGSEVNP ISLYHIIRSF DPCVSCAVHT IRTG
//
