ID AUXI2_ARATH Reviewed; 891 AA.
AC Q0WQ57; Q9SU09;
DT 13-JUL-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 27-MAR-2024, entry version 104.
DE RecName: Full=Auxilin-related protein 2;
GN OrderedLocusNames=At4g12770; ORFNames=T20K18.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
CC -!- FUNCTION: Promotes probably uncoating of clathrin-coated vesicles.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:18433157}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q0WQ57-1; Sequence=Displayed;
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB40994.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78319.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL049640; CAB40994.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161534; CAB78319.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83177.1; -; Genomic_DNA.
DR EMBL; AK228848; BAF00742.1; -; mRNA.
DR PIR; T06635; T06635.
DR RefSeq; NP_193013.2; NM_117346.4. [Q0WQ57-1]
DR AlphaFoldDB; Q0WQ57; -.
DR SMR; Q0WQ57; -.
DR BioGRID; 12186; 1.
DR STRING; 3702.Q0WQ57; -.
DR iPTMnet; Q0WQ57; -.
DR PaxDb; 3702-AT4G12770-1; -.
DR ProteomicsDB; 240932; -. [Q0WQ57-1]
DR EnsemblPlants; AT4G12770.1; AT4G12770.1; AT4G12770. [Q0WQ57-1]
DR GeneID; 826889; -.
DR Gramene; AT4G12770.1; AT4G12770.1; AT4G12770. [Q0WQ57-1]
DR KEGG; ath:AT4G12770; -.
DR Araport; AT4G12770; -.
DR TAIR; AT4G12770; AUXILIN-LIKE2.
DR eggNOG; KOG0431; Eukaryota.
DR InParanoid; Q0WQ57; -.
DR OMA; NFDEHAN; -.
DR OrthoDB; 5491812at2759; -.
DR PhylomeDB; Q0WQ57; -.
DR PRO; PR:Q0WQ57; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q0WQ57; baseline and differential.
DR Genevisible; Q0WQ57; AT.
DR GO; GO:0009504; C:cell plate; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IPI:TAIR.
DR GO; GO:1900186; P:negative regulation of clathrin-dependent endocytosis; IDA:TAIR.
DR GO; GO:0045806; P:negative regulation of endocytosis; IDA:TAIR.
DR GO; GO:0045926; P:negative regulation of growth; IDA:TAIR.
DR Gene3D; 1.10.287.110; DnaJ domain; 1.
DR InterPro; IPR036869; J_dom_sf.
DR PANTHER; PTHR23172; AUXILIN/CYCLIN G-ASSOCIATED KINASE-RELATED; 1.
DR PANTHER; PTHR23172:SF19; J DOMAIN-CONTAINING PROTEIN; 1.
DR SUPFAM; SSF46565; Chaperone J-domain; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chaperone; Coiled coil; Cytoplasm;
KW Reference proteome.
FT CHAIN 1..891
FT /note="Auxilin-related protein 2"
FT /id="PRO_0000395460"
FT DOMAIN 606..627
FT /note="R"
FT DOMAIN 826..891
FT /note="J"
FT REGION 68..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 148..538
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..720
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 454..666
FT /evidence="ECO:0000255"
FT COILED 749..793
FT /evidence="ECO:0000255"
FT COMPBIAS 148..168
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 365..379
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..448
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 461..538
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 576..642
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 654..720
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 746..764
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 891 AA; 98327 MW; 5DE008B8145F4E90 CRC64;
MDDFTGLLAR DFGLKPQGKS APMASQSNSS AADFNTFASS YSFASAAGKK SDSLPVFDDL
GRDGDDLLFR DVFSGPPPKY GSSGDSRSPS APAFDYDVMF KEPKSKSASS MPVYDKPVYD
DEDVFESIPE LKIPSTSSQS ARFENVFSSI SSSPTKHRKQ NSSPFDDLMG NNLGKKESDR
EEKGSSIFDD LIPGFGRTSS PPAKRTTSET TSQSQKPPYR TAETSSNVKE DPFVVLEEST
STLREPSTGG FTDPLEEIGK FNSRKTDHSS VHGGVFVDTD PLDSLGKSGP DMNSRGKSHL
RPPGNISGSQ SPVESSGLYH SKNVSFDDVV EPQNTSTPPP TNSDGSFESS DDVWLTVSEI
PLFTQPTSAP PPTRPPPPRP TRPIKKKVNE PSIPTSAYHS HVPSSGRASV NSPTASQMDE
LDDFSIGRNQ TAANGYPDPS SGEDSDVFST AAASAAAMKD AMDKAEAKFR HAKERREKES
LKASRSREGD HTENYDSRER ELREKQVRLD RERAEREAEM EKTQAREREE REREQKRIER
ERERLLARQA VERATREARE RAATEAHAKV QRAAVGKVTD ARERAERAAV QRAHAEARER
AAAGAREKAE KAAAEARERA NAEVREKEAK VRAERAAVER AAAEARGRAA AQAKAKQQQE
NNNDLDSFFN SVSRPSSVPR QRTNPPDPFQ DSWNKGGSFE SSRPSSRVPS GPTENLRKAS
SATNIVDDLS SIFGAPASQS GGFQDVDGET EERRRARLER HQRTQERAAK ALAEKNERDL
QVQREQAEKD RIGGTLDVEI RRWGAGKEGN LRALLSTLQY VLWPECGWQP VSLTDLITGA
SVKKVYRKAT LCIHPDKVQQ KGANLQQKYI AEKVFDMLKE AWNKFNSEEL F
//
