GenomeNet

Database: UniProt
Entry: Q10HL3
LinkDB: Q10HL3
Original site: Q10HL3 
ID   DCL2A_ORYSJ             Reviewed;        1410 AA.
AC   Q10HL3; A0A0P0VZP1; Q75IB0; Q75IB1; Q75IB2;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   16-OCT-2019, entry version 97.
DE   RecName: Full=Endoribonuclease Dicer homolog 2a;
DE   AltName: Full=Dicer-like protein 2a;
DE            Short=OsDCL2a;
DE            EC=3.1.26.-;
GN   Name=DCL2A; OrderedLocusNames=Os03g0583900, LOC_Os03g38740;
GN   ORFNames=OJ1785_A05.30;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16109971; DOI=10.1101/gr.3869505;
RG   The rice chromosome 3 sequencing consortium;
RA   Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R.,
RA   Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L.,
RA   Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S., Johri S.,
RA   Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S.,
RA   Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J.,
RA   de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H.,
RA   Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F.,
RA   Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A.,
RA   Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J.,
RA   Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L.,
RA   Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A.,
RA   Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W.,
RA   Lee H.R., Jiang J., Jackson S.;
RT   "Sequence, annotation, and analysis of synteny between rice chromosome
RT   3 and diverged grass species.";
RL   Genome Res. 15:1284-1291(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H.,
RA   McCombie W.R., Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S.,
RA   Childs K.L., Davidson R.M., Lin H., Quesada-Ocampo L.,
RA   Vaillancourt B., Sakai H., Lee S.S., Kim J., Numa H., Itoh T.,
RA   Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using
RT   next generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18826656; DOI=10.1186/1471-2164-9-451;
RA   Kapoor M., Arora R., Lama T., Nijhawan A., Khurana J.P., Tyagi A.K.,
RA   Kapoor S.;
RT   "Genome-wide identification, organization and phylogenetic analysis of
RT   dicer-like, argonaute and RNA-dependent RNA polymerase gene families
RT   and their expression analysis during reproductive development and
RT   stress in rice.";
RL   BMC Genomics 9:451-451(2008).
CC   -!- FUNCTION: Probably involved in the RNA silencing pathway. May
CC       cleave double-stranded RNA to produce short 21-24 nucleotides (nt)
CC       RNAs which target the selective destruction of complementary RNAs
CC       (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SUBUNIT: May interact with ARGONAUTE1 or PINHEAD through their
CC       common PAZ domains. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the helicase family. Dicer subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00657}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAS07188.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAS07189.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAS07190.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AC133333; AAS07188.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC133333; AAS07189.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC133333; AAS07190.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; DP000009; ABF97330.1; -; Genomic_DNA.
DR   EMBL; AP008209; BAF12478.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS85077.1; -; Genomic_DNA.
DR   EMBL; AK072334; BAG92928.1; -; mRNA.
DR   RefSeq; XP_015630302.1; XM_015774816.1.
DR   STRING; 4530.OS03T0583900-01; -.
DR   PaxDb; Q10HL3; -.
DR   EnsemblPlants; Os03t0583900-01; Os03t0583900-01; Os03g0583900.
DR   GeneID; 4333337; -.
DR   Gramene; Os03t0583900-01; Os03t0583900-01; Os03g0583900.
DR   KEGG; osa:4333337; -.
DR   eggNOG; KOG0701; Eukaryota.
DR   eggNOG; COG0571; LUCA.
DR   eggNOG; COG1111; LUCA.
DR   HOGENOM; HOG000006099; -.
DR   InParanoid; Q10HL3; -.
DR   KO; K11592; -.
DR   OMA; PTVALCE; -.
DR   OrthoDB; 1337630at2759; -.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q10HL3; baseline and differential.
DR   Genevisible; Q10HL3; OS.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0016442; C:RISC complex; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004525; F:ribonuclease III activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030422; P:production of siRNA involved in RNA interference; IBA:GO_Central.
DR   CDD; cd00593; RIBOc; 2.
DR   Gene3D; 1.10.1520.10; -; 2.
DR   Gene3D; 3.30.160.380; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR038248; Dicer_dimer_sf.
DR   InterPro; IPR005034; Dicer_dimerisation_dom.
DR   InterPro; IPR014720; dsRBD_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003100; PAZ_dom.
DR   InterPro; IPR036085; PAZ_dom_sf.
DR   InterPro; IPR000999; RNase_III_dom.
DR   InterPro; IPR036389; RNase_III_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF03368; Dicer_dimer; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF02170; PAZ; 1.
DR   Pfam; PF00636; Ribonuclease_3; 2.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00358; DSRM; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00949; PAZ; 1.
DR   SMART; SM00535; RIBOc; 2.
DR   SUPFAM; SSF101690; SSF101690; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF69065; SSF69065; 2.
DR   PROSITE; PS51327; DICER_DSRBF; 1.
DR   PROSITE; PS50137; DS_RBD; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50821; PAZ; 1.
DR   PROSITE; PS00517; RNASE_3_1; 1.
DR   PROSITE; PS50142; RNASE_3_2; 2.
PE   2: Evidence at transcript level;
KW   ATP-binding; Complete proteome; Endonuclease; Helicase; Hydrolase;
KW   Magnesium; Manganese; Metal-binding; Nuclease; Nucleotide-binding;
KW   Nucleus; Reference proteome; Repeat; RNA-binding;
KW   RNA-mediated gene silencing.
FT   CHAIN         1   1410       Endoribonuclease Dicer homolog 2a.
FT                                /FTId=PRO_0000378417.
FT   DOMAIN       41    222       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      388    561       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN      569    655       Dicer dsRNA-binding fold.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00657}.
FT   DOMAIN      815    942       PAZ. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00142}.
FT   DOMAIN      969   1124       RNase III 1. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1161   1308       RNase III 2. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00177}.
FT   DOMAIN     1334   1400       DRBM. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00266}.
FT   NP_BIND      54     61       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   MOTIF       163    166       DECH box. {ECO:0000250}.
FT   METAL      1200   1200       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1294   1294       Magnesium or manganese. {ECO:0000250}.
FT   METAL      1297   1297       Magnesium or manganese. {ECO:0000250}.
FT   SITE       1290   1290       Important for activity. {ECO:0000250}.
SQ   SEQUENCE   1410 AA;  158494 MW;  7B092E0ED9401277 CRC64;
     MGGPLTAAGG RGDGGAKAVE PLRPPPPPDP KTMARWYQLE ALERAVRGNT LAFLETGSGK
     TLIAVMLLRA YAHRVRRPDS RRFAVFLVPT VVLVGQQARV VEQHTDLVVK QFCGEMGVDF
     WDAATWRSQL EDGEVLVMTP QILLDNLRHS FFRLQDIALL IFDECHHARG NTPYACIFKE
     FYHPQLNSSA SDPLPRIFGM SASLIYSKDL NPHNYSKQIS EIENLMNSKV YTVDSESALS
     EYIPFASTKI VDFDDSNISS ELHANILSCL NRLNKKHIEA LDRKLHGSSL ENAKQRISKL
     HHTFVYCLYN LGVWLAAKAA EVQSYEENSL SFWGETLDKN VEGFIRNYSE EVHRELSCFL
     KNGHIGEKFP ADSQDGILTP KVHCLIRTLL QYRHMQDLRC IVFVERVITS IVLEHLLSSI
     HQMSGWNVKH MAGSRPGLLS QSRKNHTEIV ESFRKGKVHI IIATQILEEG LDVPSCNLVI
     RFDPSATVCS FIQSRGRARM ENSDYLLLVG RGDVEAQTNA EKFLASGQIM REESLRLGSI
     SCQPLENTLC EDTYYRVEST RAIVTLNSSV PLIHFFCSKL PSDEYFNPLP RFDIDKASGT
     CTLHLPKSSP VQTVNVEGEG SILKETVCLK ACQELHAIGA LTDSLLPELD VPCDEEPDIV
     VENKIEQPSY FPEEFVDNWR SFSRLGIYYC YKISLEGCPK TASPTDILLA LKCDLGSDFT
     SSSFKLPGGQ DNASVTMKYV GIIHLNQEQV IIARRFQTTI LSFLIGDDHL EVSNGIKYFH
     EMQVPIGVVY LLLPLVSGRI DWCSMKFSSS PIYEANNKHM THCHSCKDID LLQTKDGPFC
     RCILKNSIVC TPHNNIFYVI SGFLDLDANS CLPQHDGTVV TYKDYFKTRH GLTLTFENQP
     LLAGSKHVKV RNFLHNCYSK KEKEPGDRYS VELPPELCRI IMSPVSANNL HIFSYVPSIM
     FRIQCMLLSV KLKVQLGPTV QQFDVPVLKI LEALTTKKCQ EEFSQESLET LGDSFLKYVT
     TRHLFSEYRL QHEGILTKMK KNLISNAALC QLACSSNLVG YIHAEEFNPR DWIIPCLDYD
     ERDNKKISFL APNGMYSQRK MSIKSKRIAD SVEALIGAYL STAGEKAAFL LMKSLGMNIE
     FHTEIPVERK ISMKAEEFIN VRSLEGMLGY KFNDSLLLLE ALTHGSYQTS GPTSCYQRLE
     FLGDAILDHL FTEYYYSKYP DCTPELLTDL RSASVNNNCY AHAAVKSGLN KHILHSSSEL
     HRKMSYYLEE FGQSFTGPSY GWEAGIGLPK VLGDVIESIA GAIYLDSKCD KEVVWRSMKR
     LLEPLATPET IEPDPVKGLQ EFCDRRSFKI TYEKNHVDGV SSVIARVKAG ETTYSATKSG
     PCKLVAKKLA SKAVLKDLIA GHKDTEAAAV
//
DBGET integrated database retrieval system