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Database: UniProt
Entry: Q12039
LinkDB: Q12039
Original site: Q12039 
ID   HMI1_YEAST              Reviewed;         706 AA.
AC   Q12039; D6W1X3; Q05379;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   28-MAR-2018, entry version 140.
DE   RecName: Full=ATP-dependent DNA helicase HMI1, mitochondrial;
DE            EC=3.6.4.12;
DE   Flags: Precursor;
GN   Name=HMI1; OrderedLocusNames=YOL095C; ORFNames=O0920;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=1346062; DOI=10.1128/MCB.12.1.155;
RA   Fien K., Stillman B.;
RT   "Identification of replication factor C from Saccharomyces cerevisiae:
RT   a component of the leading-strand DNA replication complex.";
RL   Mol. Cell. Biol. 12:155-163(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7502582; DOI=10.1002/yea.320111108;
RA   Vandenbol M., Durand P., Portetelle D., Hilger F.;
RT   "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV
RT   including the Ty1-H3 retrotransposon, the suf1(+) frameshift
RT   suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a
RT   delta element.";
RL   Yeast 11:1069-1075(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8533473; DOI=10.1002/yea.320111009;
RA   Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.;
RT   "A 29.425 kb segment on the left arm of yeast chromosome XV contains
RT   more than twice as many unknown as known open reading frames.";
RL   Yeast 11:975-986(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W.,
RA   Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R.,
RA   Boyer J., Camasses A., Casamayor A., Casas C., Cheret G.,
RA   Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H.,
RA   Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F.,
RA   Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A.,
RA   Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J.,
RA   Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P.,
RA   Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M.,
RA   Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R.,
RA   Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S.,
RA   Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A.,
RA   Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M.,
RA   Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C.,
RA   Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S.,
RA   Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M.,
RA   Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and
RT   now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   FUNCTION, COFACTOR, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-704
RP   AND ARG-705.
RX   PubMed=10669756; DOI=10.1128/MCB.20.5.1816-1824.2000;
RA   Sedman T., Kuusk S., Kivi S., Sedman J.;
RT   "A DNA helicase required for maintenance of the functional
RT   mitochondrial genome in Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 20:1816-1824(2000).
RN   [7]
RP   C-TERMINAL TARGETING DOMAIN.
RX   PubMed=10409639; DOI=10.1074/jbc.274.30.20937;
RA   Lee C.M., Sedman J., Neupert W., Stuart R.A.;
RT   "The DNA helicase, Hmi1p, is transported into mitochondria by a C-
RT   terminal cleavable targeting signal.";
RL   J. Biol. Chem. 274:20937-20942(1999).
CC   -!- FUNCTION: Required for mitochondrial genome maintenance and
CC       mitochondrial DNA inheritance. {ECO:0000269|PubMed:10669756}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:10669756};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000305|PubMed:10669756}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:10669756}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA88166.1; Type=Erroneous initiation; Evidence={ECO:0000305};
DR   EMBL; U26030; AAC49064.1; -; Genomic_DNA.
DR   EMBL; Z48149; CAA88166.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X83121; CAA58184.1; -; Genomic_DNA.
DR   EMBL; Z74837; CAA99107.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10689.1; -; Genomic_DNA.
DR   PIR; S57374; S57374.
DR   RefSeq; NP_014546.1; NM_001183349.1.
DR   ProteinModelPortal; Q12039; -.
DR   SMR; Q12039; -.
DR   BioGrid; 34307; 315.
DR   DIP; DIP-4225N; -.
DR   MINT; Q12039; -.
DR   STRING; 4932.YOL095C; -.
DR   iPTMnet; Q12039; -.
DR   MaxQB; Q12039; -.
DR   PaxDb; Q12039; -.
DR   PRIDE; Q12039; -.
DR   EnsemblFungi; YOL095C; YOL095C; YOL095C.
DR   GeneID; 854058; -.
DR   KEGG; sce:YOL095C; -.
DR   EuPathDB; FungiDB:YOL095C; -.
DR   SGD; S000005455; HMI1.
DR   GeneTree; ENSGT00530000066700; -.
DR   HOGENOM; HOG000112919; -.
DR   InParanoid; Q12039; -.
DR   KO; K19781; -.
DR   OMA; KHLTIAG; -.
DR   OrthoDB; EOG092C2AXC; -.
DR   BioCyc; YEAST:G3O-33495-MONOMER; -.
DR   BRENDA; 3.6.4.12; 984.
DR   PRO; PR:Q12039; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005759; C:mitochondrial matrix; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IDA:SGD.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0032042; P:mitochondrial DNA metabolic process; IMP:SGD.
DR   GO; GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Magnesium; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Nucleotide-binding; Reference proteome.
FT   CHAIN         1    692       ATP-dependent DNA helicase HMI1,
FT                                mitochondrial.
FT                                /FTId=PRO_0000013297.
FT   PROPEP      693    706       Cleaved upon import into mitochondrion.
FT                                /FTId=PRO_0000013298.
FT   DOMAIN        5    277       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00560}.
FT   DOMAIN      278    593       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00617}.
FT   NP_BIND      29     34       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00560}.
FT   BINDING     275    275       ATP. {ECO:0000250}.
FT   MUTAGEN     704    704       R->D: Impaired import into mitochondrion.
FT                                {ECO:0000269|PubMed:10669756}.
FT   MUTAGEN     705    705       R->D: Impaired import into mitochondrion.
FT                                {ECO:0000269|PubMed:10669756}.
SQ   SEQUENCE   706 AA;  80576 MW;  626FD0FB2A5520F5 CRC64;
     MDKLTPSQWK VINKSYEPAS TIKVIAGPGS GKTLTLLYKV LHLITVENIK PEEILIFSLT
     NKAVDSIIEN LLSIFENSHT NKEIVHQIGC YTVHGLANRI VVENEGMINI IEEIGWRGLM
     KLLPPSKRTP HHFRSYKELE KVVKDYKLNN AKNNNPVIEK LVELMDNCKV MTNDDLIIRA
     KKYLELDSSD SDASSFTQDL RNKYKVVLID EFQDLYPSLA PLITMICKGK QLIMFGDTNQ
     SIYGFLGSNN EIMSQLDNLH PKNSTTVLKL FDNFRSTPEI ISLASKIINR PLAEKQIIDD
     TDETPSELVR KLPSGVSPQI MTFDDLAAES EFIIDKITQL ICSSAKFSDI AILSRTNSHL
     TAIASILKKY GIPYQKLKSQ PDWMDDLRIQ FLLDILKVCS LASDEKHNRE FNTGDKWQSN
     FSILVTMSAL KGIGDASIQA LYKACSLKNL SIWKYLTMVP NFEWPLGLSI KKKMENYTSN
     LYEMIENDQV HQLDDPMELL EKVASITNNL NLNPTYFQSL SDAQSSLEFK THLQEMAQVM
     KVSKSNKPPG ISFVKWFLET YFDQTMVFHQ SQQALQTTGP GTVKLSTIHS AKGLEFPIVF
     LTNGSMSNFP MDTNALYVGI TRARNLLYMC NMKHERLVSK SSPYSRNIMS NNLFWTYYNK
     DLKRSVCDVK VTHGYNVQRY NQLRKNFGFY RAYSSLRGCK SVFRRI
//
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