ID Q122E9_POLSJ Unreviewed; 992 AA.
AC Q122E9;
DT 22-AUG-2006, integrated into UniProtKB/TrEMBL.
DT 22-AUG-2006, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN OrderedLocusNames=Bpro_4713 {ECO:0000313|EMBL:ABE46593.1};
OS Polaromonas sp. (strain JS666 / ATCC BAA-500).
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Polaromonas.
OX NCBI_TaxID=296591 {ECO:0000313|EMBL:ABE46593.1, ECO:0000313|Proteomes:UP000001983};
RN [1] {ECO:0000313|Proteomes:UP000001983}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JS666 / ATCC BAA-500 {ECO:0000313|Proteomes:UP000001983};
RX PubMed=18723656; DOI=10.1128/AEM.00197-08;
RA Mattes T.E., Alexander A.K., Richardson P.M., Munk A.C., Han C.S.,
RA Stothard P., Coleman N.V.;
RT "The genome of Polaromonas sp. strain JS666: insights into the evolution of
RT a hydrocarbon- and xenobiotic-degrading bacterium, and features of
RT relevance to biotechnology.";
RL Appl. Environ. Microbiol. 74:6405-6416(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
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DR EMBL; CP000316; ABE46593.1; -; Genomic_DNA.
DR RefSeq; WP_011485578.1; NC_007948.1.
DR AlphaFoldDB; Q122E9; -.
DR STRING; 296591.Bpro_4713; -.
DR KEGG; pol:Bpro_4713; -.
DR eggNOG; COG0550; Bacteria.
DR eggNOG; COG5531; Bacteria.
DR HOGENOM; CLU_002929_5_2_4; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000001983; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd10567; SWIB-MDM2_like; 1.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.245.10; SWIB/MDM2 domain; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR019835; SWIB_domain.
DR InterPro; IPR036885; SWIB_MDM2_dom_sf.
DR InterPro; IPR003121; SWIB_MDM2_domain.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF02201; SWIB; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 2.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00151; SWIB; 1.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR SUPFAM; SSF47592; SWIB/MDM2 domain; 1.
DR PROSITE; PS51925; SWIB_MDM2; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:ABE46593.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000001983};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 2..139
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT DOMAIN 916..992
FT /note="DM2"
FT /evidence="ECO:0000259|PROSITE:PS51925"
FT REGION 900..920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 992 AA; 109023 MW; 67578940485BF069 CRC64;
MKTLVIAEKP SVAQDIVRAI TPTAGKFEKH DEYFESDEWL VTSAVGHLVE IQAPEEFDVK
RGKWSFANLP VIPPYFEVKP IDKTRTRLNA IVKLAKRKDV GAIVNACDAG REGELIFRLI
QQYANSKHPV KRLWLQSMTP AAIREGFDSL RSEKQMQGLA DAARSRSEAD WMVGINGTRA
MTAFNSRDGG FFLTTVGRVQ TPTLSVVVER EEKIRKFVSR DYWEIHASFL AEAGEYPAKW
FDPKWKKAAA NADNAEPDAE LRADRVWSER EALAIAEAVR GKAATVTEES KPTTQAAGLL
FDLTSLQREA NGKFGFSAKT TLSIAQSLYE RHKALTYPRT DSRALPEDYL PVVKQTFEML
ADSGMRHLAP HAATAIKGNY IKPSKRIFDN AKVSDHFAII PTTQAPSGLS EAEQKLYDLV
VRRFMAVFYP SAEYLVTTRI SQAVGYSFKT EGKVLVKPGW LAIYGKEAAD EVADAKDGDK
GQSLVPVKPG EMVRAEVVEA KGLKTRPPAR YSEATLLGAM EGAGKTIDDD ELREAMQEKG
LGTPATRAAT IEGLIAEKYM LREGRELIPT AKAFQLMTLL RGLGVEELSK AELTGEWEYK
LAQMEHGKLS RETFMAEIAA MTENLVNKAK GYDKDTIPGD YTTLQTPCPN CGGVVKENYR
RYTCTGKNGG DGCGFSFGKT PAGRTFETAE VEQFLRDRKI GPLEGFRSKA GWPFTAEMVI
KFDEETKNYK LEFDFGDDKN GETGEIVDFG DQQSLGPCPR CAARVFELGK NYVCEKSVPT
LEQPTPSCTF KTGQVILQQP IEREQMHKLL ETGKTDLLDK FVSMRTRRPF KAMLAWDKEA
DKVNFEFAPS KFPPRKPAAA KTGVVKTPFG KTVAAKGAAG DAAPAAKKAA AKKVAAKKAP
AKTAAPKVAR KAATPKPGAG LKPSDSLAAV IGAEPVARTQ VIKKLWDYIK AEGLQDAANK
RAINADAKLL PVFGKPQVTM FELAGIVGKH LS
//
