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Database: UniProt
Entry: Q1JMH5
LinkDB: Q1JMH5
Original site: Q1JMH5 
ID   ADDA_STRPC              Reviewed;        1222 AA.
AC   Q1JMH5;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   10-OCT-2018, entry version 85.
DE   RecName: Full=ATP-dependent helicase/nuclease subunit A {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01451};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_01451};
DE   AltName: Full=ATP-dependent helicase/nuclease AddA {ECO:0000255|HAMAP-Rule:MF_01451};
GN   Name=addA {ECO:0000255|HAMAP-Rule:MF_01451}; Synonyms=rexA;
GN   OrderedLocusNames=MGAS9429_Spy0649;
OS   Streptococcus pyogenes serotype M12 (strain MGAS9429).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=370551;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGAS9429;
RX   PubMed=16636287; DOI=10.1073/pnas.0510279103;
RA   Beres S.B., Richter E.W., Nagiec M.J., Sumby P., Porcella S.F.,
RA   DeLeo F.R., Musser J.M.;
RT   "Molecular genetic anatomy of inter- and intraserotype variation in
RT   the human bacterial pathogen group A Streptococcus.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:7059-7064(2006).
CC   -!- FUNCTION: The heterodimer acts as both an ATP-dependent DNA
CC       helicase and an ATP-dependent, dual-direction single-stranded
CC       exonuclease. Recognizes the chi site generating a DNA molecule
CC       suitable for the initiation of homologous recombination. The AddA
CC       nuclease domain is required for chi fragment generation; this
CC       subunit has the helicase and 3' -> 5' nuclease activities.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01451};
CC   -!- SUBUNIT: Heterodimer of AddA and AddB/RexB. {ECO:0000255|HAMAP-
CC       Rule:MF_01451}.
CC   -!- SIMILARITY: Belongs to the helicase family. AddA subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01451}.
DR   EMBL; CP000259; ABF31837.1; -; Genomic_DNA.
DR   ProteinModelPortal; Q1JMH5; -.
DR   PRIDE; Q1JMH5; -.
DR   EnsemblBacteria; ABF31837; ABF31837; MGAS9429_Spy0649.
DR   KEGG; spk:MGAS9429_Spy0649; -.
DR   HOGENOM; HOG000285114; -.
DR   KO; K16898; -.
DR   OMA; KQSIYRW; -.
DR   Proteomes; UP000002433; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.320.10; -; 1.
DR   HAMAP; MF_01451; AddA; 1.
DR   InterPro; IPR014152; DNA_helicase_suAddA.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR011604; Exonuc_phg/RecB_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR038726; PDDEXK_AddAB-type.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   InterPro; IPR034739; UvrD/AddA_N.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   PANTHER; PTHR11070:SF14; PTHR11070:SF14; 1.
DR   Pfam; PF12705; PDDEXK_1; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 3.
DR   SUPFAM; SSF52980; SSF52980; 1.
DR   TIGRFAMs; TIGR02785; addA_Gpos; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; DNA damage; DNA repair; DNA-binding;
KW   Exonuclease; Helicase; Hydrolase; Nuclease; Nucleotide-binding.
FT   CHAIN         1   1222       ATP-dependent helicase/nuclease subunit
FT                                A.
FT                                /FTId=PRO_0000379342.
FT   DOMAIN       39    495       UvrD-like helicase ATP-binding.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   DOMAIN      524    810       UvrD-like helicase C-terminal.
FT                                {ECO:0000255|HAMAP-Rule:MF_01451}.
FT   NP_BIND      60     67       ATP. {ECO:0000255|HAMAP-Rule:MF_01451}.
SQ   SEQUENCE   1222 AA;  140225 MW;  C8AB3791491CEFF7 CRC64;
     MLFNINEKGE PLVISFAPFL SPEAIKHLQE NERCSDQSQK RTAQQIEAIY TSGQNILVSA
     SAGSGKTFVM VERILDKILR GVSIDRLFIS TFTVKAATEL RERIENKLYS QIAQTTDFQM
     KVYLTEQLQS LGQADIGTMD AFAQKVVSRY GYSIGISSQF RIMQDKAEQD VLKQEVFSKL
     FSEFMNQKEA PVFRALVKNF SGNCKDTSAF RELVYTCYSF SQSTENPKIW LQENFLSAAK
     TYQRLEDIPD HDIELLLLAM QDTANQLRDV TDMEDYGQLT KAGSRSAKYT KHLTIIEKLS
     DWVRDFKCLY GKAGLDRLIR DVTGLIPSGN DVTVSKVKYP VFKTLHQKLK QFRHLETILM
     YQKDCFPLLE QLQDFVLAFS EAYLAVKIQE SAFEFSDITH FAIKILEENT DIRQSYQQHY
     HEVMVDEYQD NNHMQERLLT LLSNGHNRFM VGDIKQSIYR FRQADPQIFN QKFRDYQKKT
     EQGKVILLKE NFRSQSEVLN VSNAVFSHLM DESVGDVLYD EQHQLIAGSH AQTVPYLDRR
     AQLLLYNSDK DDGNAPSDSE GISFSEVTIV AKEIIKLHND KGVPFEDITL LVSSRTRNDI
     ISHTFNQYGI PIVTDGGQQN YLKSVEVMVM LDTLRTINNP RNDYALVALL RSPMFAFDED
     DLARIALQKD NELDKDCLYD KIQRAVIGRG AHPELIHDTL LGKLNIFLKT LKSWRRYAKL
     GSLYDLIWKI FNDRFYFDFV ASQAKAEQAQ ANLYALALRA NQFEKSGYKG LYCFIKMIDK
     VLETQNDLAD VEVAAPKQAV NLMTIHKSKG LQFPYVFILN CDKRFSMTDI HKSFILNRQH
     GIGIKYLADI KGLLGETTLN SVKVSMETLP YQLNKQELRL ATLSEQMRLL YVAMTRAEKK
     VYFIGKASKS KSQDITDPKK LGKLLPLALR EQLLTFQDWL LAIADVFSTE DLYFDVRFIE
     DSDLTQESVG RLQTPQLLNP DDLKDNRQSE TIARALDMLE AVSQLNANYE AAIHLPTVRT
     PSQLKAAYEP LLEPIGVDII EKSSRSLSDF TLPHFSKKVK VEASHIGSAL HQLMQVLPLS
     KPINQQTLLD ALREIDSNEE VKTALDLKKI ESFFCDTSLG QFFQTYQKHL YREAPFAILK
     VDPISQEEYV LRGIIDAYFL FDDHIVLVDY KTDKYKQPIE LKKRYQQQLE LYAEALTQTY
     KLPVTKRYLV LMGGGKPEIV EV
//
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