ID Q1LWX7_DANRE Unreviewed; 832 AA.
AC Q1LWX7; B3DKM5;
DT 30-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 30-MAY-2006, sequence version 1.
DT 27-MAR-2024, entry version 129.
DE RecName: Full=Bromodomain-containing protein 2 {ECO:0000256|ARBA:ARBA00040998};
GN Name=brd2a {ECO:0000313|EMBL:AAI63902.1,
GN ECO:0000313|Ensembl:ENSDARP00000139243,
GN ECO:0000313|RefSeq:NP_001257500.1,
GN ECO:0000313|ZFIN:ZDB-GENE-990415-248};
GN Synonyms=brd2 {ECO:0000313|EMBL:CAK04960.1,
GN ECO:0000313|RefSeq:NP_001257500.1}, fe47f02
GN {ECO:0000313|RefSeq:NP_001257500.1}, fi85h05
GN {ECO:0000313|RefSeq:NP_001257500.1}, ring3
GN {ECO:0000313|RefSeq:NP_001257500.1}, wu:fi85h05
GN {ECO:0000313|RefSeq:NP_001257500.1};
GN ORFNames=CH211-51F10.8-001 {ECO:0000313|EMBL:CAK04960.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|EMBL:AAI63902.1};
RN [1] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=17055577; DOI=10.1016/j.dci.2006.07.010;
RA Palti Y., Rodriguez M.F., Gahr S.A., Hansen J.D.;
RT "Evolutionary history of the ABCB2 genomic region in teleosts.";
RL Dev. Comp. Immunol. 31:483-498(2007).
RN [2] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=18402692;
RA Dibenedetto A.J., Guinto J.B., Ebert T.D., Bee K.J., Schmidt M.M.,
RA Jackman T.R.;
RT "Zebrafish brd2a and brd2b are paralogous members of the bromodomain-ET
RT (BET) family of transcriptional coregulators that show structural and
RT expression divergence.";
RL BMC Dev. Biol. 8:39-39(2008).
RN [3] {ECO:0000313|EMBL:AAI63902.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:CAK04960.1}
RP NUCLEOTIDE SEQUENCE.
RA Howden P.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=21346254;
RA de Jong J.L., Burns C.E., Chen A.T., Pugach E., Mayhall E.A., Smith A.C.,
RA Feldman H.A., Zhou Y., Zon L.I.;
RT "Characterization of immune-matched hematopoietic transplantation in
RT zebrafish.";
RL Blood 117:4234-4242(2011).
RN [6] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=24240475;
RA Huang H.T., Kathrein K.L., Barton A., Gitlin Z., Huang Y.H., Ward T.P.,
RA Hofmann O., Dibiase A., Song A., Tyekucheva S., Hide W., Zhou Y., Zon L.I.;
RT "A network of epigenetic regulators guides developmental haematopoiesis in
RT vivo.";
RL Nat. Cell Biol. 15:1516-1525(2013).
RN [7] {ECO:0000313|Ensembl:ENSDARP00000139243, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000139243};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [8] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25010335;
RA Bielczyk-Maczynska E., Serbanovic-Canic J., Ferreira L., Soranzo N.,
RA Stemple D.L., Ouwehand W.H., Cvejic A.;
RT "A loss of function screen of identified genome-wide association study Loci
RT reveals new genes controlling hematopoiesis.";
RL PLoS Genet. 10:E1004450-E1004450(2014).
RN [9] {ECO:0000313|Ensembl:ENSDARP00000139243}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000139243};
RG Ensembl;
RL Submitted (NOV-2015) to UniProtKB.
RN [10] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27189481;
RA Pasquier J., Cabau C., Nguyen T., Jouanno E., Severac D., Braasch I.,
RA Journot L., Pontarotti P., Klopp C., Postlethwait J.H., Guiguen Y.,
RA Bobe J.;
RT "Gene evolution and gene expression after whole genome duplication in fish:
RT the PhyloFish database.";
RL BMC Genomics 17:368-368(2016).
RN [11] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=27493218;
RA McConnell S.C., Hernandez K.M., Wcisel D.J., Kettleborough R.N.,
RA Stemple D.L., Yoder J.A., Andrade J., de Jong J.L.;
RT "Alternative haplotypes of antigen processing genes in zebrafish diverged
RT early in vertebrate evolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 113:E5014-E5023(2016).
RN [12] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=28549975;
RA Murphy T., Melville H., Fradkin E., Bistany G., Branigan G., Olsen K.,
RA Comstock C.R., Hanby H., Garbade E., DiBenedetto A.J.;
RT "Knockdown of epigenetic transcriptional co-regulator Brd2a disrupts
RT apoptosis and proper formation of hindbrain and midbrain-hindbrain boundary
RT (MHB) region in zebrafish.";
RL Mech. Dev. 146:10-30(2017).
RN [13] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=29488319;
RA Kindt L.M., Coughlin A.R., Perosino T.R., Ersfeld H.N., Hampton M.,
RA Liang J.O.;
RT "Identification of transcripts potentially involved in neural tube closure
RT using RNA sequencing.";
RL Genesis 56:e23096-e23096(2018).
RN [14] {ECO:0000313|RefSeq:NP_001257500.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=30523256;
RA Reisser M., Palmer A., Popp A.P., Jahn C., Weidinger G., Gebhardt J.C.M.;
RT "Single-molecule imaging correlates decreasing nuclear volume with
RT increasing TF-chromatin associations during zebrafish development.";
RL Nat. Commun. 9:5218-5218(2018).
RN [15] {ECO:0000313|RefSeq:NP_001257500.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBUNIT: Homodimer. Interacts with E2F1 and with histone H4 acetylated
CC at 'Lys-13'. {ECO:0000256|ARBA:ARBA00038820}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; FP085392; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC163902; AAI63902.1; -; mRNA.
DR EMBL; BC163905; AAI63905.1; -; mRNA.
DR EMBL; BX510994; CAK04960.1; -; Genomic_DNA.
DR RefSeq; NP_001257500.1; NM_001270571.1.
DR RefSeq; NP_571275.2; NM_131200.2.
DR Ensembl; ENSDART00000160894.2; ENSDARP00000139243.1; ENSDARG00000022280.12.
DR GeneID; 30445; -.
DR KEGG; dre:30445; -.
DR AGR; ZFIN:ZDB-GENE-990415-248; -.
DR CTD; 30445; -.
DR ZFIN; ZDB-GENE-990415-248; brd2a.
DR OrthoDB; 152619at2759; -.
DR Proteomes; UP000000437; Chromosome 19.
DR Bgee; ENSDARG00000022280; Expressed in pharyngeal gill and 70 other cell types or tissues.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0070577; F:lysine-acetylated histone binding; IBA:GO_Central.
DR GO; GO:0006338; P:chromatin remodeling; IBA:GO_Central.
DR GO; GO:0030902; P:hindbrain development; IMP:ZFIN.
DR GO; GO:0030917; P:midbrain-hindbrain boundary development; IMP:ZFIN.
DR GO; GO:0042981; P:regulation of apoptotic process; IMP:ZFIN.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IBA:GO_Central.
DR GO; GO:0021510; P:spinal cord development; IMP:ZFIN.
DR GO; GO:0002574; P:thrombocyte differentiation; IMP:ZFIN.
DR CDD; cd05497; Bromo_Brdt_I_like; 1.
DR CDD; cd05498; Bromo_Brdt_II_like; 1.
DR Gene3D; 1.20.1270.220; -; 1.
DR Gene3D; 1.20.920.10; Bromodomain-like; 2.
DR InterPro; IPR043508; Bromo_Brdt_I.
DR InterPro; IPR043509; Bromo_Brdt_II.
DR InterPro; IPR001487; Bromodomain.
DR InterPro; IPR036427; Bromodomain-like_sf.
DR InterPro; IPR018359; Bromodomain_CS.
DR InterPro; IPR027353; NET_dom.
DR InterPro; IPR038336; NET_sf.
DR PANTHER; PTHR22880:SF240; BROMODOMAIN-CONTAINING PROTEIN 2; 1.
DR PANTHER; PTHR22880; FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS; 1.
DR Pfam; PF17035; BET; 1.
DR Pfam; PF00439; Bromodomain; 2.
DR PRINTS; PR00503; BROMODOMAIN.
DR SMART; SM00297; BROMO; 2.
DR SUPFAM; SSF47370; Bromodomain; 2.
DR PROSITE; PS00633; BROMODOMAIN_1; 2.
DR PROSITE; PS50014; BROMODOMAIN_2; 2.
DR PROSITE; PS51525; NET; 1.
PE 1: Evidence at protein level;
KW Bromodomain {ECO:0000256|ARBA:ARBA00023117, ECO:0000256|PROSITE-
KW ProRule:PRU00035};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q1LWX7};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 89..161
FT /note="Bromo"
FT /evidence="ECO:0000259|PROSITE:PS50014"
FT DOMAIN 398..470
FT /note="Bromo"
FT /evidence="ECO:0000259|PROSITE:PS50014"
FT DOMAIN 663..745
FT /note="NET"
FT /evidence="ECO:0000259|PROSITE:PS51525"
FT REGION 52..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 257..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 343..380
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..682
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 770..832
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 349..370
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 504..531
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 570..589
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 590..604
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 612..631
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 660..682
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 803..832
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 832 AA; 91988 MW; AD981E57CAEE827A CRC64;
METALSPPFD SSLGGCDGMQ GLTMMEQTIS GPGKRIRKPS LLYEGFEGPA LPHIAQSGPP
QPAVRDPSRQ GRMTNQLQFL QKALVKTLWR HHFAWPFHEP VDAAKLNLPD YYNIIKQPMD
MGTIKKRLEN NYYRSASECM QDFNTMFTNC YIYNKPTDDI VLMAQSLEKA FLQKVAQMPE
VEEEIPAPVP RGKQSKPKKG QKLFSGGITT AHQVPAISSL SQLAYSPPTP DTPDSVLSTP
PQTLITKNFP PPPLHSTPAL LGLPPTQPTA KKKGVKRKAD TTTPTTTVMP LTLGVGGMGM
GMGMGMSMGM GMGMGLGGDS PLTLSNMGMD SKQSLSLSLG MSLEDKVPAR RGGSGRPIKP
PRKDLPDSQN QHQPVRRGKL SQQLRYCSTI LKELLSKKHT AYAWPFYKPV DVSSLGLHDY
YDIIMYPMDL STIKRKMDHR EYRDALQFAA DVRLMFSNCY KYNPPDHDVV AMARRLQDVF
EFRFAKMPDE PLESLPPASL GGGLGGDSSS SSSSSSSSSE SDVSSESESE SSPSSDSEEE
RAHRLAQLQE QLRAVHEQLA ALSSTPIIKP KKKKEKKDKK KKKKPEKHKR SRSVIEDEVV
MRPAKIPKLP KTPKSRSNSK AATSTQSKKS SNKKSNKSKS SKKAQPTFFP PPLSHSGLTP
HYDSEEEEET SPMSYDEKRQ LSLDINRLPG EKLGRVVHII QSREPSLRDT NPEEIEIDFE
TLKPSTLREL ERYVMMCLRK KPRKPFVAIK GSAGKSREEL ALEKKRELER RLQDVSGQLN
SVKKPQKTKA EKPSAVEPHA VASRLSASSS SSDSSSSSSS SSSSDTSESD SD
//
