ID Q1LX46_DANRE Unreviewed; 1012 AA.
AC Q1LX46; A0A8M1N9Z5;
DT 30-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 30-MAY-2006, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE SubName: Full=Solute carrier family 12 member 3 {ECO:0000313|Ensembl:ENSDARP00000025500, ECO:0000313|RefSeq:NP_001038545.1};
GN Name=slc12a3 {ECO:0000313|Ensembl:ENSDARP00000025500,
GN ECO:0000313|RefSeq:NP_001038545.1,
GN ECO:0000313|ZFIN:ZDB-GENE-030131-9505};
GN Synonyms=si:ch211-122c9.4 {ECO:0000313|RefSeq:NP_001038545.1},
GN wu:fj83c03 {ECO:0000313|RefSeq:NP_001038545.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000025500};
RN [1] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.D., Sun X.J., Deng M., Zhang G.W., Zhou Y., Wu X.Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.L., Fan H.Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [2] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=17953490;
RA Wingert R.A., Selleck R., Yu J., Song H.D., Chen Z., Song A., Zhou Y.,
RA Thisse B., Thisse C., McMahon A.P., Davidson A.J.;
RT "The cdx genes and retinoic acid control the positioning and segmentation
RT of the zebrafish pronephros.";
RL PLoS Genet. 3:1922-1938(2007).
RN [3] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=18391953;
RA Ji W., Foo J.N., O'Roak B.J., Zhao H., Larson M.G., Simon D.B.,
RA Newton-Cheh C., State M.W., Levy D., Lifton R.P.;
RT "Rare independent mutations in renal salt handling genes contribute to
RT blood pressure variation.";
RL Nat. Genet. 40:592-599(2008).
RN [4] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=19279294;
RA Wang Y.F., Tseng Y.C., Yan J.J., Hiroi J., Hwang P.P.;
RT "Role of SLC12A10.2, a Na-Cl cotransporter-like protein, in a Cl uptake
RT mechanism in zebrafish (Danio rerio).";
RL Am. J. Physiol. Regul. Integr. Comp. Physiol. 296:R1650-R1660(2009).
RN [5] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=19855023;
RA Fauny J.D., Thisse B., Thisse C.;
RT "The entire zebrafish blastula-gastrula margin acts as an organizer
RT dependent on the ratio of Nodal to BMP activity.";
RL Development 136:3811-3819(2009).
RN [6] {ECO:0000313|Ensembl:ENSDARP00000025500, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000025500};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [7] {ECO:0000313|Ensembl:ENSDARP00000025500}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000025500};
RG Ensembl;
RL Submitted (AUG-2013) to UniProtKB.
RN [8] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=30475208;
RA Chambers J.M., Poureetezadi S.J., Addiego A., Lahne M., Wingert R.A.;
RT "ppargc1a controls nephron segmentation during zebrafish embryonic kidney
RT ontogeny.";
RL Elife 7:e40266-e40266(2018).
RN [9] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=30515132; DOI=10.3389/fendo.2018.00683;
RA Shu T., Shu Y., Gao Y., Jin X., He J., Zhai G., Yin Z.;
RT "Depletion of Tissue-Specific Ion Transporters Causes Differential
RT Expression of PRL Targets in Response to Increased Levels of Endogenous
RT PRL.";
RL Front. Endocrinol. 9:683-683(2018).
RN [10] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=30575756;
RA Morales E.E., Handa N., Drummond B.E., Chambers J.M., Marra A.N.,
RA Addiego A., Wingert R.A.;
RT "Homeogene emx1 is required for nephron distal segment development in
RT zebrafish.";
RL Sci. Rep. 8:18038-18038(2018).
RN [11] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=30948642;
RA Marra A.N., Adeeb B.D., Chambers B.E., Drummond B.E., Ulrich M.,
RA Addiego A., Springer M., Poureetezadi S.J., Chambers J.M., Ronshaugen M.,
RA Wingert R.A.;
RT "Prostaglandin signaling regulates renal multiciliated cell specification
RT and maturation.";
RL Proc. Natl. Acad. Sci. U.S.A. 116:8409-8418(2019).
RN [12] {ECO:0000313|RefSeq:NP_001038545.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RX PubMed=31015532;
RA Marra A.N., Cheng C.N., Adeeb B., Addiego A., Wesselman H.M.,
RA Chambers B.E., Chambers J.M., Wingert R.A.;
RT "Iroquois transcription factor irx2a is required for multiciliated and
RT transporter cell fate decisions during zebrafish pronephros development.";
RL Sci. Rep. 9:6454-6454(2019).
RN [13] {ECO:0000313|RefSeq:NP_001038545.1}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038545.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR EMBL; BX470170; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001038545.1; NM_001045080.1.
DR STRING; 7955.ENSDARP00000025500; -.
DR PaxDb; 7955-ENSDARP00000127693; -.
DR Ensembl; ENSDART00000010998; ENSDARP00000025500; ENSDARG00000013855.
DR Ensembl; ENSDART00000010998.9; ENSDARP00000025500.8; ENSDARG00000013855.12.
DR GeneID; 565439; -.
DR KEGG; dre:565439; -.
DR AGR; ZFIN:ZDB-GENE-030131-9505; -.
DR CTD; 6559; -.
DR ZFIN; ZDB-GENE-030131-9505; slc12a3.
DR eggNOG; KOG2083; Eukaryota.
DR HOGENOM; CLU_001883_0_0_1; -.
DR OMA; CQTEPTY; -.
DR OrthoDB; 5490251at2759; -.
DR PhylomeDB; Q1LX46; -.
DR TreeFam; TF313191; -.
DR Reactome; R-DRE-426117; Cation-coupled Chloride cotransporters.
DR Proteomes; UP000000437; Chromosome 18.
DR Bgee; ENSDARG00000013855; Expressed in head kidney and 23 other cell types or tissues.
DR GO; GO:0016324; C:apical plasma membrane; IBA:GO_Central.
DR GO; GO:0008511; F:sodium:potassium:chloride symporter activity; IBA:GO_Central.
DR GO; GO:0006884; P:cell volume homeostasis; IBA:GO_Central.
DR GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0035844; P:cloaca development; IMP:ZFIN.
DR GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; IBA:GO_Central.
DR GO; GO:0039023; P:pronephric duct morphogenesis; IMP:ZFIN.
DR GO; GO:0055078; P:sodium ion homeostasis; IBA:GO_Central.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002948; SLC12A3.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF9; SOLUTE CARRIER FAMILY 12 MEMBER 3; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 2.
DR PRINTS; PR01230; NACLTRNSPORT.
PE 1: Evidence at protein level;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q1LX46};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 118..139
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 151..177
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 197..221
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 242..261
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 267..288
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 322..340
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 434..458
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 488..505
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 517..534
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 554..587
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 34..105
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 123..628
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 637..778
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT DOMAIN 804..1011
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..20
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1012 AA; 111233 MW; DA9586F77B4A2E19 CRC64;
MNLQSARSPF SVSSLSNGDD RKFSLGGCSS YDTDSVASRS SGVFSGYDTL DAPPSYDFYT
NTEVFGRAKK SRPSLFELHS NPQDDPSPPP LYEESSVDKQ SPEDEDEPTE PPPEPARFGW
AQGVMIRCML NIWGVILYLR LPWITAQAGI GLTWIIILVS SSITGITGLS TSAIATNGKV
KGGGTYFLIS RSLGPELGGS IGLIFAFANA VAVAMHTVGF AETVQVLMQE TEVSMVDKLN
DIRIIGVITV TCLLAISMAG MEWESKAQVL FFFVIMISFA SYIIGTIIPA TPQKQARGFF
SYRADIFATN FVPGWRGPEG SFFGMFSIFF PSATGILAGA NISGDLKDPN VAIPRGTMLA
IFWTTVSYLI ISATIGSCVV RDASGDVNDT ISSLTGECLG VGCNYGWNFT DCMTNNTCTY
GLSNYYQSMS MVSAVAPLIT AGIFGATLSS ALACLVSAPK VFQCLCKDKL YPGIGFFGKG
YGKNNEPLRS YLLAYIIAIC FILIAELNTI APIISNFFLC SYALINFSCF HASITNSPGW
RPTFRFYSKW LSLLGAVVSV IIMFLLTWWA ALIAIGIVIF LLGYVLYKKP EVNWGSSMQA
SSYNMALSQC VGLNQVEDHI KNYRPQCLVL SGPPCARPSL VDFIGAFTKN QSLMICANVL
ASGPSPGTAD SMSSTHLKWL NNRKIKSFYH TVVADDLRTG VQMLLQSTGL GRMKPNVLVM
GYKKNWRKVQ PGIIENYVGI LHDAFDLQYG VCVLRMKEGL DITRTIQAQV NLGFETSTEQ
GLDTNSTAPT SPTIEASLDP ETLMALTQPS TLFQTRQGKK TIDVYWLSDD GGLTLLIPYL
LTRKKRWGRC KVRVFVGGEA QQIEEQKKEL KGLISRFRLG FKDIQVLPDI NGAPQSEHIR
KFEDFIAPYR VSSVQKDGQE ADEATKEFSW MVSDEEMETF KAKSLRQIRL NEVIQDYSRD
AALIVVTMPV GRRGSCPSPL YMAWLEIVSR DLRPPVLLVR GNQENVLTQY CQ
//
