ID Q1LYG5_DANRE Unreviewed; 854 AA.
AC Q1LYG5; A0A8M1NA43;
DT 30-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 20-FEB-2007, sequence version 2.
DT 27-MAR-2024, entry version 137.
DE SubName: Full=Eukaryotic translation elongation factor 2a, tandem duplicate 1 {ECO:0000313|Ensembl:ENSDARP00000118068, ECO:0000313|RefSeq:NP_001038626.2};
GN Name=eef2a.1 {ECO:0000313|Ensembl:ENSDARP00000118068,
GN ECO:0000313|RefSeq:NP_001038626.2,
GN ECO:0000313|ZFIN:ZDB-GENE-050522-500};
GN Synonyms=si:dkey-110c1.3 {ECO:0000313|RefSeq:NP_001038626.2},
GN zgc:113191 {ECO:0000313|RefSeq:NP_001038626.2};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000118068};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000118068, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000118068};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000313|Ensembl:ENSDARP00000118068}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000118068};
RG Ensembl;
RL Submitted (AUG-2013) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:NP_001038626.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038626.2};
RX PubMed=25260251;
RA Hatef A., Shajan S., Unniappan S.;
RT "Nutrient status modulates the expression of nesfatin-1 encoding
RT nucleobindin 2A and 2B mRNAs in zebrafish gut, liver and brain.";
RL Gen. Comp. Endocrinol. 215:51-60(2015).
RN [4] {ECO:0000313|RefSeq:NP_001038626.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038626.2};
RX PubMed=26469318;
RA Elkon R., Milon B., Morrison L., Shah M., Vijayakumar S., Racherla M.,
RA Leitch C.C., Silipino L., Hadi S., Weiss-Gayet M., Barras E., Schmid C.D.,
RA Ait-Lounis A., Barnes A., Song Y., Eisenman D.J., Eliyahu E.,
RA Frolenkov G.I., Strome S.E., Durand B., Zaghloul N.A., Jones S.M.,
RA Reith W., Hertzano R.;
RT "RFX transcription factors are essential for hearing in mice.";
RL Nat. Commun. 6:8549-8549(2015).
RN [5] {ECO:0000313|RefSeq:NP_001038626.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038626.2};
RX PubMed=26226634;
RA Hatef A., Yufa R., Unniappan S.;
RT "Ghrelin O-Acyl Transferase in Zebrafish Is an Evolutionarily Conserved
RT Peptide Upregulated During Calorie Restriction.";
RL Zebrafish 12:327-338(2015).
RN [6] {ECO:0000313|RefSeq:NP_001038626.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038626.2};
RX PubMed=28252024;
RA Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A.,
RA Choudhary J.S., Emes R.D., Grant S.G.;
RT "Evolution of complexity in the zebrafish synapse proteome.";
RL Nat. Commun. 8:14613-14613(2017).
RN [7] {ECO:0000313|RefSeq:NP_001038626.2}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:NP_001038626.2};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189;
CC Evidence={ECO:0000256|ARBA:ARBA00001702};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19670;
CC Evidence={ECO:0000256|ARBA:ARBA00001702};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; BX005203; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001038626.2; NM_001045161.2.
DR STRING; 7955.ENSDARP00000118068; -.
DR PaxDb; 7955-ENSDARP00000118068; -.
DR Ensembl; ENSDART00000143641; ENSDARP00000118068; ENSDARG00000042094.
DR Ensembl; ENSDART00000143641.3; ENSDARP00000118068.1; ENSDARG00000042094.8.
DR GeneID; 792182; -.
DR KEGG; dre:792182; -.
DR AGR; ZFIN:ZDB-GENE-050522-500; -.
DR CTD; 792182; -.
DR ZFIN; ZDB-GENE-050522-500; eef2a.1.
DR eggNOG; KOG0469; Eukaryota.
DR HOGENOM; CLU_002794_11_2_1; -.
DR OrthoDB; 166721at2759; -.
DR PhylomeDB; Q1LYG5; -.
DR TreeFam; TF300575; -.
DR Reactome; R-DRE-156902; Peptide chain elongation.
DR Reactome; R-DRE-5358493; Synthesis of diphthamide-EEF2.
DR Reactome; R-DRE-6798695; Neutrophil degranulation.
DR Reactome; R-DRE-8876725; Protein methylation.
DR Proteomes; UP000000437; Alternate scaffold 22.
DR Proteomes; UP000000437; Chromosome 22.
DR Bgee; ENSDARG00000042094; Expressed in gastrula and 26 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0003746; F:translation elongation factor activity; IBA:GO_Central.
DR GO; GO:0006414; P:translational elongation; IBA:GO_Central.
DR CDD; cd01681; aeEF2_snRNP_like_IV; 1.
DR CDD; cd04096; eEF2_snRNP_like_C; 1.
DR CDD; cd01885; EF2; 1.
DR CDD; cd16261; EF2_snRNP_III; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.70.240; -; 1.
DR Gene3D; 3.30.70.870; Elongation Factor G (Translational Gtpase), domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR005517; Transl_elong_EFG/EF2_IV.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR42908:SF29; EUKARYOTIC TRANSLATION ELONGATION FACTOR 2B-RELATED; 1.
DR PANTHER; PTHR42908; TRANSLATION ELONGATION FACTOR-RELATED; 1.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF03764; EFG_IV; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SMART; SM00889; EFG_IV; 1.
DR SUPFAM; SSF54980; EF-G C-terminal domain-like; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 1: Evidence at protein level;
KW Elongation factor {ECO:0000313|RefSeq:NP_001038626.2};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q1LYG5};
KW Reference proteome {ECO:0000313|Proteomes:UP000000437}.
FT DOMAIN 17..360
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 854 AA; 94978 MW; E98DDD2D4F59B149 CRC64;
MENFNVDQIR ATMDKKSNIR NMSVIGAFDH GKSTLTDWLV SEAGIVSSAR AGETRFMDTR
RDEQERCITI KSTAISIFYE LAEKDLAFIK ECKDGSGFLL NLIDSPGHVD FSSEVTAALR
ITDGALLVVD CVSGVCLQTE TVLRQAIGER IKPVLMINKM DRALLELQLV PEELYQIFQR
IVEKVNVTIS TYAEDEKGPM GNVMIDPVIG NLAFGSGLHG WAFTLKQFAE LYVKKFAGKA
QLGPEEYIKK VEDMIKRLWG DSYFDSTTGK FSESATSPDG KKLPRTFVHL VLDPIFKVFD
AIMNFKKEET VKLIEKMGIK LDVEDKEKEG KILLKAVMRC WLPAGEALLQ MITIHLPSPV
TAQIYRCELL YEGPGDDEAA MGIKNCDPKA PLMMYISKMI PTSDKGCFYA FGRVFSGCVS
TGLNVRIMGP NFTPGKKDDL YLKPIQRTVL MMGRYVEPIE DVPCGNIVGL LGVDQFLVKT
GTITTFTKAY NMRVMKFSVS PVVRVSVEVM DPADLPKLVE GLKHLAKSDP MLQCIIEDTG
EHIIAGAGEL HLEICLKDLE EDHACIPLKK SDPFASYRET VSDGSKQLCL AKTPNKHSRL
FMKACPLADG LAEDIDKGRV TASQEMNARA QYLAENYKWE VTEARKIWCF GPEGTGPNIL
VDLTKRVQYL NEIKDSVIAG FQWATREGVL CAENMRGIRF DIHDATLTST AIHRGPGQII
VATRRVLYGC QLTAEPRLSE PIYLVEMQCP ESVIGNVYGE LVRRRGVVFS ESQVMGTPVY
LLKAYLPVSE SFGFTADLCA NTSGQAFSQC VFDHWQILPG DPMDPTSKVA HIMADIRKSK
GLDEAIPALH CYLD
//
