ID Q1PUK7_KUEST Unreviewed; 587 AA.
AC Q1PUK7;
DT 16-MAY-2006, integrated into UniProtKB/TrEMBL.
DT 16-MAY-2006, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=ftsH {ECO:0000313|EMBL:CAJ70914.1};
GN Synonyms=fhs {ECO:0000256|HAMAP-Rule:MF_01543,
GN ECO:0000313|EMBL:QII13314.1}, ftsH_6 {ECO:0000313|EMBL:SOH05655.1};
GN ORFNames=KsCSTR_39350 {ECO:0000313|EMBL:QII13314.1}, KSMBR1_3178
GN {ECO:0000313|EMBL:SOH05655.1}, kustb0169
GN {ECO:0000313|EMBL:CAJ70914.1};
OS Kuenenia stuttgartiensis.
OC Bacteria; Planctomycetota; Candidatus Brocadiia; Candidatus Brocadiales;
OC Candidatus Brocadiaceae; Kuenenia.
OX NCBI_TaxID=174633 {ECO:0000313|EMBL:CAJ70914.1};
RN [1] {ECO:0000313|EMBL:CAJ70914.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=16598256; DOI=10.1038/nature04647;
RA Strous M., Pelletier E., Mangenot S., Rattei T., Lehner A., Taylor M.W.,
RA Horn M., Daims H., Bartol-Mavel D., Wincker P., Barbe V., Fonknechten N.,
RA Vallenet D., Segurens B., Schenowitz-Truong C., Medigue C., Collingro A.,
RA Snel B., Dutilh B.E., OpDenCamp H.J.M., vanDerDrift C., Cirpus I.,
RA vanDePas-Schoonen K.T., Harhangi H.R., vanNiftrik L., Schmid M.,
RA Keltjens J., vanDeVossenberg J., Kartal B., Meier H., Frishman D.,
RA Huynen M.A., Mewes H., Weissenbach J., Jetten M.S.M., Wagner M.,
RA LePaslier D.;
RT "Deciphering the evolution and metabolism of an anammox bacterium from a
RT community genome.";
RL Nature 440:790-794(2006).
RN [2] {ECO:0000313|EMBL:CAJ70914.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SOH05655.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kuenenia_mbr1_ru-nijmegen {ECO:0000313|EMBL:SOH05655.1};
RA Banno H., Chua N.-H.;
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|Proteomes:UP000221734}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Frank J.;
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:QII13314.1, ECO:0000313|Proteomes:UP000501926}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CSTR1 {ECO:0000313|EMBL:QII13314.1,
RC ECO:0000313|Proteomes:UP000501926};
RA Ding C., Adrian L.;
RT "Newly sequenced genome of strain CSTR1 showed variability in Candidatus
RT Kuenenia stuttgartiensis genomes.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; CT573074; CAJ70914.1; -; Genomic_DNA.
DR EMBL; CP049055; QII13314.1; -; Genomic_DNA.
DR EMBL; LT934425; SOH05655.1; -; Genomic_DNA.
DR AlphaFoldDB; Q1PUK7; -.
DR KEGG; kst:KSMBR1_3178; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000221734; Chromosome Kuenenia_stuttgartiensis_MBR1.
DR Proteomes; UP000501926; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:CAJ70914.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000221734}.
FT BINDING 74..81
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 587 AA; 63482 MW; F29DDAAC7E764847 CRC64;
MSLDPTKFAD WQIAEEAEKG MKTVYELADK LGLAKEELLP HGHYVAKVDY KKILKRYEGK
PDGKFVDVTA ITPTPLGEGK STTTIGLVQG LGKRGKNVIG TIRQPSGGPT MNIKGSAAGG
GLSQCIPLTP FSLGLTGDIN AIMNAHNLAM VALTSRMQHE FNYDDATLAK KNLRRLNIDP
HRVEMKWIID FCAQSLRQIV IGLGGKMDGF MMESGFAIAV SSEIMAILSV AKDLKDMRER
MGKIVVAYDK SGKPVTTADL EVDGAMTAWM VEALNPNLMQ TIEGQPVFVH AGPFANIAIG
QSSIVADRIA LKLGDYVVTE SGFGADIGFE KFWNLKCRFS GSKPNAAVLV ATVRALKCHG
GAPVPVPGHP LDPCYKEENV GWVEKGTENL THLINVVKKA GINPVVCINA FYTDTEPEIN
AVRRLAERAG ARVALSKHWL KGGDGAVELA DAVIDACNEP NDFKFLYELS TPLRTRIELI
AEQVYGADGV DYSPDALAKV KAMEADPETS KLGTCMVKTH LSLTDNPNIK GAPKNWRLSI
RDILTFKGAG FVVPIAGNIK LMPGTCSDPA YRRVDVDVET GKVKGLF
//
