ID Q20240_CAEEL Unreviewed; 460 AA.
AC Q20240;
DT 01-NOV-1996, integrated into UniProtKB/TrEMBL.
DT 02-SEP-2008, sequence version 2.
DT 27-MAR-2024, entry version 132.
DE RecName: Full=tRNA wybutosine-synthesizing protein 2 homolog {ECO:0000256|ARBA:ARBA00017179};
DE EC=2.5.1.114 {ECO:0000256|ARBA:ARBA00012265};
DE AltName: Full=tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase {ECO:0000256|ARBA:ARBA00031315};
GN ORFNames=CELE_F40F9.10 {ECO:0000313|EMBL:CAA94765.2}, F40F9.10
GN {ECO:0000313|EMBL:CAA94765.2, ECO:0000313|WormBase:F40F9.10};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000313|EMBL:CAA94765.2, ECO:0000313|Proteomes:UP000001940};
RN [1] {ECO:0000313|EMBL:CAA94765.2, ECO:0000313|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000313|EMBL:CAA94765.2,
RC ECO:0000313|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RA Sulson J.E., Waterston R.;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent transferase that acts as a
CC component of the wybutosine biosynthesis pathway. Wybutosine is a hyper
CC modified guanosine with a tricyclic base found at the 3'-position
CC adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes
CC the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-
CC adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14)
CC to produce wybutosine-86. {ECO:0000256|ARBA:ARBA00037786}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine =
CC 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) +
CC H(+) + S-methyl-5'-thioadenosine; Xref=Rhea:RHEA:36355, Rhea:RHEA-
CC COMP:10164, Rhea:RHEA-COMP:10378, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:59789, ChEBI:CHEBI:64315,
CC ChEBI:CHEBI:73550; EC=2.5.1.114;
CC Evidence={ECO:0000256|ARBA:ARBA00036405};
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DR EMBL; BX284605; CAA94765.2; -; Genomic_DNA.
DR RefSeq; NP_505502.2; NM_073101.4.
DR AlphaFoldDB; Q20240; -.
DR SMR; Q20240; -.
DR STRING; 6239.F40F9.10.1; -.
DR EPD; Q20240; -.
DR PaxDb; 6239-F40F9-10; -.
DR EnsemblMetazoa; F40F9.10.1; F40F9.10.1; WBGene00009586.
DR GeneID; 179363; -.
DR KEGG; cel:CELE_F40F9.10; -.
DR UCSC; F40F9.10; c. elegans.
DR AGR; WB:WBGene00009586; -.
DR WormBase; F40F9.10; CE42760; WBGene00009586; -.
DR eggNOG; KOG1227; Eukaryota.
DR GeneTree; ENSGT00940000153304; -.
DR HOGENOM; CLU_022610_1_0_1; -.
DR InParanoid; Q20240; -.
DR OMA; ELLCCQQ; -.
DR OrthoDB; 122704at2759; -.
DR PhylomeDB; Q20240; -.
DR PRO; PR:Q20240; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00009586; Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0102522; F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008175; F:tRNA methyltransferase activity; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0031591; P:wybutosine biosynthetic process; IBA:GO_Central.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 3.30.300.110; Met-10+ protein-like domains; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030382; MeTrfase_TRM5/TYW2.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR23245; TRNA METHYLTRANSFERASE; 1.
DR PANTHER; PTHR23245:SF25; TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 2 HOMOLOG; 1.
DR Pfam; PF02475; Met_10; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51684; SAM_MT_TRM5_TYW2; 1.
PE 1: Evidence at protein level;
KW Proteomics identification {ECO:0007829|EPD:Q20240};
KW Reference proteome {ECO:0000313|Proteomes:UP000001940};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 51..307
FT /note="SAM-dependent methyltransferase TRM5/TYW2-type"
FT /evidence="ECO:0000259|PROSITE:PS51684"
FT REGION 260..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..298
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 320..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 460 AA; 52532 MW; 78DBCCFA0594BAC3 CRC64;
MERTSSLRRK TSDGVLAKAS NFGQMLEDVR KLALSKSLWD DEMQRDLPKK WEKHGDMIVF
PQNTFTHINW RYIGRELWAV VAQSLNVARV GRKRLIDEER TPHVDLLLGA DAWVDYVDER
GIKFCYDATK RVFENSKKAE MKRISKWSCQ GQTIVDMYAS LGYYTLTFLV SCEAKQVVAI
DWNDEILESL IRSAQVNNVD DRLLVIHGDC RRVCPDQTAD RVYLGLLPSC RAHWLAACKA
LKPEGGIVHI NEILDMTEKK KTPQKTEKVE KKVVAEKKKK PADGKEKTDK DAAPKKKKVL
RQKTLPVLSA VHEDSKPEVE STSIPTAPES DEPSENNSET NGTEGGQKKK KFSRSASIVE
EMENRVLPEV KIWKEYENEG WARLTDRHRE FAKDCADSCT RFLNNIHLSD LMYSVTVVNM
IRYGEVSKGK EHIVLELLCC QQDASAEHLI SKFTSENLKS
//
