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Database: UniProt
Entry: Q20497
LinkDB: Q20497
Original site: Q20497 
ID   MED12_CAEEL             Reviewed;        3498 AA.
AC   Q20497;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 2.
DT   16-JAN-2019, entry version 118.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=CeTRAP230;
DE   AltName: Full=Mediator complex subunit 12;
DE   AltName: Full=Protein dumpy-22;
GN   Name=dpy-22; Synonyms=mdt-12, psa-6, sop-1; ORFNames=F47A4.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=10970880; DOI=10.1101/gad.814700;
RA   Zhang H., Emmons S.W.;
RT   "A C. elegans mediator protein confers regulatory selectivity on
RT   lineage-specific expression of a transcription factor gene.";
RL   Genes Dev. 14:2161-2172(2000).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=12441291; DOI=10.1242/dev.00189;
RA   Moghal N., Sternberg P.W.;
RT   "A component of the transcriptional mediator complex inhibits RAS-
RT   dependent vulval fate specification in C. elegans.";
RL   Development 130:57-69(2003).
RN   [4]
RP   FUNCTION.
RX   PubMed=15073178; DOI=10.1074/jbc.M401242200;
RA   Wang J.-C., Walker A., Blackwell T.K., Yamamoto K.R.;
RT   "The Caenorhabditis elegans ortholog of TRAP240, CeTRAP240/let-19,
RT   selectively modulates gene expression and is essential for
RT   embryogenesis.";
RL   J. Biol. Chem. 279:29270-29277(2004).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLU-1040.
RX   PubMed=15790964; DOI=10.1242/dev.01776;
RA   Yoda A., Kouike H., Okano H., Sawa H.;
RT   "Components of the transcriptional Mediator complex are required for
RT   asymmetric cell division in C. elegans.";
RL   Development 132:1885-1893(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator
CC       involved in regulated gene transcription of nearly all RNA
CC       polymerase II-dependent genes. Mediator functions as a bridge to
CC       convey information from gene-specific regulatory proteins to the
CC       basal RNA polymerase II transcription machinery. Mediator is
CC       recruited to promoters by direct interactions with regulatory
CC       proteins and serves as a scaffold for the assembly of a functional
CC       preinitiation complex with RNA polymerase II and the general
CC       transcription factors (By similarity). Functions downstream of
CC       let-60 during vulval induction. Required for asymmetric division
CC       of T-cells and for hypodermal development. {ECO:0000250,
CC       ECO:0000269|PubMed:10970880, ECO:0000269|PubMed:12441291,
CC       ECO:0000269|PubMed:15073178, ECO:0000269|PubMed:15790964}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10970880,
CC       ECO:0000269|PubMed:12441291, ECO:0000269|PubMed:15790964}.
CC   -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC       {ECO:0000269|PubMed:10970880}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in vulval precursor cells, the
CC       anchor cell and the hyp7 cell at the time of vulval fate
CC       specification and throughout vulval development. Expressed from
CC       the 8-cell stage of embryogenesis. {ECO:0000269|PubMed:10970880}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
DR   EMBL; Z49888; CAA90064.1; -; Genomic_DNA.
DR   PIR; T22330; T22330.
DR   RefSeq; NP_509645.1; NM_077244.3.
DR   UniGene; Cel.459; -.
DR   ProteinModelPortal; Q20497; -.
DR   BioGrid; 46109; 15.
DR   STRING; 6239.F47A4.2; -.
DR   EPD; Q20497; -.
DR   PaxDb; Q20497; -.
DR   PeptideAtlas; Q20497; -.
DR   PRIDE; Q20497; -.
DR   EnsemblMetazoa; F47A4.2; F47A4.2; WBGene00001081.
DR   GeneID; 181194; -.
DR   KEGG; cel:CELE_F47A4.2; -.
DR   UCSC; F47A4.2; c. elegans.
DR   CTD; 181194; -.
DR   WormBase; F47A4.2; CE16058; WBGene00001081; dpy-22.
DR   eggNOG; KOG3598; Eukaryota.
DR   eggNOG; ENOG410XP2V; LUCA.
DR   GeneTree; ENSGT00440000037505; -.
DR   HOGENOM; HOG000113519; -.
DR   InParanoid; Q20497; -.
DR   KO; K15162; -.
DR   OMA; ICEQDWV; -.
DR   OrthoDB; 66382at2759; -.
DR   SignaLink; Q20497; -.
DR   PRO; PR:Q20497; -.
DR   Proteomes; UP000001940; Chromosome X.
DR   Bgee; WBGene00001081; Expressed in 5 organ(s), highest expression level in material anatomical entity.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:WormBase.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0007275; P:multicellular organism development; IEA:UniProtKB-KW.
DR   GO; GO:0042059; P:negative regulation of epidermal growth factor receptor signaling pathway; IGI:WormBase.
DR   GO; GO:0040027; P:negative regulation of vulval development; IGI:WormBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019035; Mediator_Med12.
DR   InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR   Pfam; PF09497; Med12; 1.
DR   Pfam; PF12145; Med12-LCEWAV; 1.
DR   SMART; SM01281; Med12; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; Complete proteome; Developmental protein;
KW   Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN         1   3498       Mediator of RNA polymerase II
FT                                transcription subunit 12.
FT                                /FTId=PRO_0000312962.
FT   REGION     2142   3498       Required for nuclear localization.
FT   COILED      539    570       {ECO:0000255}.
FT   COILED     2203   2290       {ECO:0000255}.
FT   COILED     2395   2420       {ECO:0000255}.
FT   COMPBIAS   2554   2714       Glu-rich.
FT   COMPBIAS   2718   3497       Gln-rich.
FT   MUTAGEN    1040   1040       E->K: In os26; impairs asymmetric
FT                                division of T-cells.
FT                                {ECO:0000269|PubMed:15790964}.
SQ   SEQUENCE   3498 AA;  400779 MW;  283FFC65A6B9E336 CRC64;
     MLRKTNLRMG TADVYKQNKN QEEDMLTTER LKKGYQMPLT QYELQSLVFN ASENKLIRLE
     KAQKRSQSMI ASVIHKKGEF HMNYDRERKI PKEQLPCFAP HNWKRILSDR VKFFSALARG
     TPLQQLSQRI PGFRRKELLF LEFSDYNISY DRAIWCVKLL CILGFNSSSK TQKKTLLDVA
     TLELSQACNK TTIWLVTQLY KSFRNPIKRR EATKTWNYLT GFMKYMFDDG VLDKQEFLND
     LTEVFNQLFL QKNFKNPSVL STFMKYYVYF VEDLSSSLVL ARRSSTLLCQ ALGFLFEMAQ
     DKRKIDAYYD SANKTFIKKE FENEDGEIYE FEDYDDFIDM LDEIPDVPYF DETDKTPEAQ
     AEELIKQHEK RKAGSLKKSE RRRRRGLSKN RPKKMPQNPS NEASLDHDKV QIKQEPMDYD
     EYGGQQSDNS MDYDSFSHQY DPSYEPLPFK PLHTDIPKKL TDDEEFKYMK IGKRSVTPER
     ISGRYGSMTY PFWIQQGGVG IDPPKDEVLD FSNPTPTETV NTQPQIDFES SPSASPALSV
     DKENECEKKE DESKTKEKNK DKEKDKEKEK SVDEHTNDLD VPINPDDAEM ADANDKTDAS
     EKQKLVEEEP TGKENEDDTS SKTAKTSTSA EKSEAPSIVD SNDKIDKEPN ASSTSNDETS
     KDDTVPMESD PPAATEKPKE STEITTEEPL EVDKAPEVDK SEKEHEDDIM IIESNKKADE
     DDCVVIAVVD PDQEQTPESE KKKDGEEERD KNKDTDVADN EPEKIYTDEE LAEIKIRKEK
     EMKIATLASH IKEKMVSDKK WKEKTSHAGW RTLDQCSQFS EALHLLSSMV QYMACVTPES
     FVWNDLSVQQ EERRHRILPQ LCGSPLDYLP CELHKLPVME GVEEVVDCLR LRHCEIVRRS
     QAAEDRWLPN AAFLQSFGRI IDTCVDVIGI MDNIDVEKPN AITNAGLRLF AFREKFEKQE
     ALLKTMLMFK WCITEEREGS FRATYIAKLL RFGMDQNPEN TIGGWQVMDL FFKFMSTEGP
     KHGSKMYQAH FDSTVAIMIE MMREKIFSIT DILRELEKDS DLDYNAPLME RQRKQKIPKI
     SKRHRKPETP DDTKLVHFTT EYTPKRLFMG KKMDLLERMI IILPQLDVDE DTDEYRLRRL
     LLFGLKPAAN VYFRRARAIY KSITKEFTTR LYIEFDRSSK VTTAHKKINQ NRLDDLLRQF
     RAQTYHDQHL ILERIVYNFI DGIGGFLKKN CDDVPAPEVA NIICEMYEFS MDITSIFDFF
     EMVNPYLKAV DDKIAHFRMD VLPDMYYTET AFIFVSFFMK HWQRFLLHPR ACAIVNQCFV
     LIQDMIRADD HMITCWGRTV AIFVFHARKA IANAGLQNEE FLAEDSHFWR VFPNAQHVDL
     DVGYFNEDFA GVKLQLRGGT LRYDSYNDFK WLVSNMKPNL KKKPHLKRPN TRYSFVVRAF
     MEARQHGRNF DRINELANYC ANITANDPPL SEYWIGAIKG LCFLSLDAPY PFKEMSQQID
     ISDCSTHYSL TTFITCLAGK SAFYIPRLLA ELTKHVFPLM LRHDGRLTSQ KTHDVKRKVA
     SKTTESRTLS EAEPGVCLCL LIICGLCCVG DEPFGLSVHY RGIEKKKKRF NNTADERIMH
     LFHWFEMDHA MFRTLGHISQ LLEALQSRCR DANLVLPKNF PIKNPPKHQQ ELHREKAPYR
     PQYLFNIAKT VQFVICEQDW VTLRMFRFFQ TRKMEAFNQD KLKQNCLGQQ ILRMALRRRT
     ERENVHKLFE AHKISKKATV DKVLSFMNLW NFRATLFDLM LMIKEISPDG NSRHAQQGAI
     AADALMSEIG KCCRDMFLSA YKTKIKMPIA KTLTDFRLSD INKFWLIAPL VRMCPKPINI
     PPQYANTTVG TVAAKFLREA ALLMDTPPTT PKERLLQCSW AMSKVPFINM ILTCLQCEKM
     QQSKDVFLQS LYTQLQRETL RDHHRRSNWT NRREHRDCTI FRITLIGYIY KEILKATHVE
     TWGLLIFQLM FHGIINPVRE KLIYETCFDM LHHMVLWTLV DGDSMNQHDR YGSIRVRWPQ
     YAGLMKKIRK EMQERFTDQT RNSLHRFLPI GKMQMSTISY KKYQKRPKVN QKMSKKFLAG
     EGLKNGKYSF LPEEKAKTNA FEHTDHLGDL IVKGGWKFRM FQTTRLDKVA KNVQNVLRSN
     MHHTHVLEFN RPQLLMSDNM FDDIFLAPPD IEITKIIEQP VPVIDEEEAK KRAEEEKEAA
     EKKEESKNAE DEKNKNNAEN KKDTKEGEKG KSKDKEKDGE KEKCKDASKK DDVTSEKNEL
     EKRASDAAAA TNAPETNKDM DTSTPKPAPV TRSPATRGRG GGRKRNSGAR GGGPRAKRAN
     SRADTAQAAA ATTQWNAPIA NTSNPAAGGN FHAAMRGNQP PMSNGSSDET KVHIRNLLNR
     KKEEKRNSLA DASAAAAAAN SNAMGNTSSM PPSGPPMPMG SSMQSAGATQ QLQGMQKHQM
     GGSMSGMNQN MGGMNQSMSH QAPPPYSSTN EMNRPLMNQY GGPHFAAPNP GPLNRSSGPV
     SSETRQQIME QQMREKLAAH HQLVEQQKQR DAREREARER EAREHQERMQ REAYMKEQQL
     LERKRAIEEN NRIMEEQQRE REMEAARKEA ARRAAEEAYA AEQQRLELLR RQEEERLRKE
     AEERMRIQRE NEERVRQEQM RLEAEERERI RRAEEERIQK ELEDKVRREK EEAARQEKER
     QEQEARMREA REAELSRQRM EQQRRSQQNP YMNQQGQYSQ QPPPSYQQSS YPNNYQPGQQ
     GNQPPNYQQP SHQSMQQGHQ AGYQQTSNQM QMNMQQQQNR QQGGPQQSFS GPGGINQPSQ
     PGYSGYNQQG GQGQQGQMQQ QRNPFGNQQD MQQPGAAKLM HAKPNEAHAQ QYQHTQNQLS
     LAQKEKEKQY FQAKNLQASQ ANAQQQQQRF GDVVAGNVAG YGRPYGQQQL GASDQMGTSQ
     LPGASTSRMN QGSSNPQGGM QSYQQQQPVL GQPGPIQTGQ STQQQIPAQS QQQYNSGRPQ
     MHTTPTKNDM SARAPSGAMG QIANRMGHGT NPQGYGSTGQ NVPGGYQQGQ QQSGQGSYPQ
     AQQQQPNQYS GSNQQVGQQA QQQQQQPLNQ NVSQSQSAAQ FGRPSQDSAY QQSGYNQTGN
     QSYQRPDQQQ QGAQQNQWSG SNQAQNQLRS QQQAQQPLQQ PQQSQQFQQP AQQAKNPMAQ
     SAQYGGFGGQ QQGYDQQQQG QIAPQQAQNP QASQSYGQQQ TQQNRYGMGS SGYTANSGGS
     SNILNQSMEE SGLNQGFSGA SSNASSQQGG SSQMQQSGYG MPGNQMQMQQ NQKQQVQRGM
     PTGMGQTNMG QSGMGQSGMG QTGMSRSGLG GGIGQQGQQS QQPQQPQVSQ QQNQRGMNPG
     AQLPPYSTGQ QQHQPQQSQI SQQQQQQDQY RRMQAAQMQQ QPTAQGQQNR MGMPSQQQSG
     AAYSNQMQFQ GVRQGQQGMG GMGGSGQQQP QTQPHGSNQY YQQQQDQRMQ QQPQQPGQQQ
     QHGYGMGQYP NQQPPNQY
//
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