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Database: UniProt
Entry: Q20958
LinkDB: Q20958
Original site: Q20958 
ID   NAS29_CAEEL             Reviewed;         532 AA.
AC   Q20958; Q7Z0M3;
DT   04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 4.
DT   16-JAN-2019, entry version 126.
DE   RecName: Full=Zinc metalloproteinase nas-29;
DE            EC=3.4.24.- {ECO:0000250|UniProtKB:A8Q2D1};
DE   AltName: Full=Nematode astacin 29;
DE   Flags: Precursor;
GN   Name=nas-29; ORFNames=F58A6.4;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 197-286, GENE STRUCTURE, AND
RP   NOMENCLATURE.
RC   STRAIN=Bristol N2;
RX   PubMed=14653817; DOI=10.1046/j.1432-1033.2003.03891.x;
RA   Moehrlen F., Hutter H., Zwilling R.;
RT   "The astacin protein family in Caenorhabditis elegans.";
RL   Eur. J. Biochem. 270:4909-4920(2003).
CC   -!- FUNCTION: Metalloprotease. {ECO:0000250|UniProtKB:A8Q2D1}.
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU01211};
CC       Note=Binds 1 zinc ion per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU01211};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
DR   EMBL; FO080690; CCD65842.1; -; Genomic_DNA.
DR   EMBL; AJ561218; CAD99218.1; -; mRNA.
DR   PIR; T16493; T16493.
DR   RefSeq; NP_494953.3; NM_062552.4.
DR   UniGene; Cel.14942; -.
DR   ProteinModelPortal; Q20958; -.
DR   SMR; Q20958; -.
DR   MEROPS; M12.A29; -.
DR   PaxDb; Q20958; -.
DR   PRIDE; Q20958; -.
DR   EnsemblMetazoa; F58A6.4; F58A6.4; WBGene00003547.
DR   GeneID; 186488; -.
DR   KEGG; cel:CELE_F58A6.4; -.
DR   UCSC; F58A6.4; c. elegans.
DR   CTD; 186488; -.
DR   WormBase; F58A6.4; CE42691; WBGene00003547; nas-29.
DR   eggNOG; KOG3714; Eukaryota.
DR   eggNOG; ENOG410ZPX7; LUCA.
DR   GeneTree; ENSGT00940000169152; -.
DR   HOGENOM; HOG000017076; -.
DR   InParanoid; Q20958; -.
DR   KO; K08076; -.
DR   OMA; NYPATIW; -.
DR   OrthoDB; 681837at2759; -.
DR   PhylomeDB; Q20958; -.
DR   PRO; PR:Q20958; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00003547; Expressed in 3 organ(s), highest expression level in multi-cellular organism.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0018996; P:molting cycle, collagen and cuticulin-based cuticle; IEA:InterPro.
DR   CDD; cd00041; CUB; 1.
DR   CDD; cd04280; ZnMc_astacin_like; 1.
DR   Gene3D; 2.60.120.290; -; 1.
DR   InterPro; IPR034035; Astacin-like_dom.
DR   InterPro; IPR000859; CUB_dom.
DR   InterPro; IPR013032; EGF-like_CS.
DR   InterPro; IPR017050; Metallopeptidase_nem.
DR   InterPro; IPR001506; Peptidase_M12A.
DR   InterPro; IPR006026; Peptidase_Metallo.
DR   InterPro; IPR035914; Sperma_CUB_dom_sf.
DR   Pfam; PF01400; Astacin; 1.
DR   Pfam; PF00431; CUB; 1.
DR   PIRSF; PIRSF036365; Astacin_nematoda; 1.
DR   PRINTS; PR00480; ASTACIN.
DR   SMART; SM00042; CUB; 1.
DR   SMART; SM00235; ZnMc; 1.
DR   SUPFAM; SSF49854; SSF49854; 1.
DR   PROSITE; PS51864; ASTACIN; 1.
DR   PROSITE; PS01180; CUB; 1.
DR   PROSITE; PS00022; EGF_1; 1.
DR   PROSITE; PS01186; EGF_2; 1.
DR   PROSITE; PS00142; ZINC_PROTEASE; 1.
PE   2: Evidence at transcript level;
KW   Cleavage on pair of basic residues; Complete proteome; Disulfide bond;
KW   EGF-like domain; Glycoprotein; Hydrolase; Metal-binding;
KW   Metalloprotease; Protease; Reference proteome; Secreted; Signal; Zinc;
KW   Zymogen.
FT   SIGNAL        1     22       {ECO:0000255}.
FT   PROPEP       23    134       {ECO:0000250|UniProtKB:P13497}.
FT                                /FTId=PRO_0000442676.
FT   CHAIN       135    532       Zinc metalloproteinase nas-29.
FT                                /FTId=PRO_0000028933.
FT   DOMAIN      135    335       Peptidase M12A. {ECO:0000255|PROSITE-
FT                                ProRule:PRU01211}.
FT   DOMAIN      330    370       EGF-like.
FT   DOMAIN      380    494       CUB. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00059}.
FT   ACT_SITE    231    231       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       230    230       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       234    234       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   METAL       240    240       Zinc; via tele nitrogen; catalytic.
FT                                {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   CARBOHYD      5      5       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     27     27       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD     70     70       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    106    106       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   CARBOHYD    503    503       N-linked (GlcNAc...) asparagine.
FT                                {ECO:0000255}.
FT   DISULFID    179    334       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   DISULFID    201    222       {ECO:0000255|PROSITE-ProRule:PRU01211}.
FT   DISULFID    338    358       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    360    369       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    380    408       {ECO:0000255|PROSITE-ProRule:PRU00059}.
FT   DISULFID    435    456       {ECO:0000255|PROSITE-ProRule:PRU00059}.
SQ   SEQUENCE   532 AA;  60395 MW;  29FBD634CB685A08 CRC64;
     MISKNTSFCG FLILVLATCM SAQFVSNESI KLHDILKPSA THRLFDTLQY SVEEQYSDSH
     LSFDVSTIYN YSEKPISIGK LNKKYRDILF EGDMAISYKQ LSMIVNGSTE YRKAIKSRRR
     GNKINGESTD RTKRQAYLDN NYPATIWKNG VAFMFHESLT PIAKTAILKA VHFWYRETCI
     EFHPRTFQKE YLLFIGNDDG CWSTVGRDAS QGKQVVSIGN GCEHFGVTSH ELAHALGIFH
     EQSRFDRDES VVFNPRVVER DLLFNFAKIS PRQMSTYGLP YDIGSVMHYT PTEFSNIPSI
     PTLAAIDTNL QQTMGQLEGP SFVDVHIMNQ HYQCQEKCPT QAPCQNGGFT NSRNCKVCKC
     PTGFGGAYCQ LIASSFSPFC GGYLNAEETT RRFDITIRQS TTTRSKTCVY HIKAPEGKRI
     IIDILKIDSK CIEGCWQDGL ELKMKKDFRP VGYRFCCPES SRRKVISEGN MVPFMVFSKE
     HDFSVSFEYS FVSTSAGFDD EKNDSDVIVD NLDGVFVSDT SLLQRIGFRR QL
//
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