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Database: UniProt
Entry: Q21Q74
LinkDB: Q21Q74
Original site: Q21Q74 
ID   KITH_RHOFT              Reviewed;         197 AA.
AC   Q21Q74;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   16-JAN-2019, entry version 76.
DE   RecName: Full=Thymidine kinase {ECO:0000255|HAMAP-Rule:MF_00124};
DE            EC=2.7.1.21 {ECO:0000255|HAMAP-Rule:MF_00124};
GN   Name=tdk {ECO:0000255|HAMAP-Rule:MF_00124};
GN   OrderedLocusNames=Rfer_4385;
OS   Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)
OS   (Albidiferax ferrireducens).
OG   Plasmid plasmid1.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Rhodoferax.
OX   NCBI_TaxID=338969;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-621 / DSM 15236 / T118;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F.,
RA   Land M., Kyrpides N., Ivanova N., Richardson P.;
RT   "Complete sequence of plasmid 1 of Rhodoferax ferrireducens DSM
RT   15236.";
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + thymidine = ADP + dTMP + H(+);
CC         Xref=Rhea:RHEA:19129, ChEBI:CHEBI:15378, ChEBI:CHEBI:17748,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:63528, ChEBI:CHEBI:456216;
CC         EC=2.7.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_00124};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00124}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00124}.
CC   -!- SIMILARITY: Belongs to the thymidine kinase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00124}.
DR   EMBL; CP000268; ABD72071.1; -; Genomic_DNA.
DR   RefSeq; WP_011458668.1; NC_007901.1.
DR   ProteinModelPortal; Q21Q74; -.
DR   SMR; Q21Q74; -.
DR   PRIDE; Q21Q74; -.
DR   EnsemblBacteria; ABD72071; ABD72071; Rfer_4385.
DR   KEGG; rfr:Rfer_4385; -.
DR   HOGENOM; HOG000076391; -.
DR   KO; K00857; -.
DR   OMA; KEQFGWI; -.
DR   OrthoDB; 1279539at2; -.
DR   Proteomes; UP000008332; Plasmid pDSM15236.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004797; F:thymidine kinase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00124; Thymidine_kinase; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001267; Thymidine_kinase.
DR   PANTHER; PTHR11441; PTHR11441; 1.
DR   Pfam; PF00265; TK; 1.
DR   PIRSF; PIRSF035805; TK_cell; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Cytoplasm; DNA synthesis; Kinase;
KW   Metal-binding; Nucleotide-binding; Plasmid; Reference proteome;
KW   Transferase; Zinc.
FT   CHAIN         1    197       Thymidine kinase.
FT                                /FTId=PRO_0000242801.
FT   NP_BIND       9     16       ATP. {ECO:0000255|HAMAP-Rule:MF_00124}.
FT   NP_BIND      83     86       ATP. {ECO:0000255|HAMAP-Rule:MF_00124}.
FT   ACT_SITE     84     84       Proton acceptor. {ECO:0000255|HAMAP-
FT                                Rule:MF_00124}.
FT   METAL       141    141       Zinc. {ECO:0000255|HAMAP-Rule:MF_00124}.
FT   METAL       143    143       Zinc. {ECO:0000255|HAMAP-Rule:MF_00124}.
FT   METAL       178    178       Zinc. {ECO:0000255|HAMAP-Rule:MF_00124}.
FT   METAL       181    181       Zinc. {ECO:0000255|HAMAP-Rule:MF_00124}.
SQ   SEQUENCE   197 AA;  21403 MW;  C082B6826ACF3A1F CRC64;
     MSQLTFIFAA MNAGKSTLLL QAAHNYVERG MAVELYTAAL DTRAGLGVIR SRLGIERGAS
     TFNADTVFDH RILERDTACL LIDESQFLRP VQVRQLHRLA HTSQVPIRCY GLRSDFLGHP
     FAGSAALLTL ADEFEEVRAV CGCGKKATMN ARLDATGARV SAGEQTLIGG NERYIAMCPR
     CFYLDSAGEQ DAQSTAA
//
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